A5026172990- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Immune Cell Function and Interaction
- Animal Disease Management and Epidemiology
- T-cell and Retrovirus Studies
- HIV-related health complications and treatments
- Vector-Borne Animal Diseases
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- COVID-19 Clinical Research Studies
- Bacteriophages and microbial interactions
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Mosquito-borne diseases and control
- Respiratory viral infections research
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Hepatitis C virus research
- Alzheimer's disease research and treatments
- Virology and Viral Diseases
Victoria University
2021-2024
Footscray Hospital
2021-2024
Ben Taub Hospital
2020
Columbia University
2020
China National GeneBank
2018-2019
Beijing Institute of Genomics
2019
Cooperative Trials Group for Neuro-Oncology
2019
Westmead Institute
2008-2018
Westmead Hospital
2008-2018
BGI Group (China)
2018
In this study we date the introduction of HIV-2 into human population and estimate epidemic history subtype A in Guinea-Bissau, putative geographic origin HIV-2. The evolutionary simian immunodeficiency virus sooty mangabey /HIV-2 lineage was reconstructed by using available database sequences with known sampling dates, a timescale for calculated maximum likelihood methods. most recent common ancestor strains estimated to be 1940 ± 16 that B 1945 14. addition used coalescent theory past...
MicroRNAs (miRNAs) are 21 to approximately 25-nucleotides (nt) long and interact with mRNAs trigger either translational repression or RNA cleavage through interference (RNAi), depending on the degree of complementarity target mRNAs. Our recent study has shown that HIV-1 nef dsRNA from AIDS patients who long-term non-progressors (LTNPs) inhibited transcription HIV-1.Here, we show possibility nef-derived miRNAs produced in persistently infected cells. Furthermore, short hairpin (shRNA)...
T and B cell receptors (TCRs BCRs) play a pivotal role in the adaptive immune system by recognizing an enormous variety of external internal antigens. Understanding these is critical for exploring process immunoreaction exploiting potential applications immunotherapy antibody drug design. Although large number samples have had their TCR BCR repertoires sequenced using high-throughput sequencing recent years, very few databases been constructed to store kinds data. To resolve this issue, we...
ABSTRACT The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number SARS cases have occurred in China, raising the possibility future epidemics. We describe here rapid, sensitive, highly efficient assay detection coronavirus (SARS-CoV) cultured material small ( n = 7) clinical samples. Using rolling circle amplification (RCA), we were able to achieve sensitive levels SARS-CoV RNA...
AIDS dementia complex (ADC) in human immunodeficiency virus (HIV)-infected patients continues to be a problem the era of highly active antiretroviral therapy (HAART). A better understanding drug resistance mutation patterns that emerge central nervous system (CNS) during HAART is paramount importance as these differences mutations may explain underlying reasons for poor penetration drugs into CNS and suboptimal concentrations reside brains HIV-infected individuals therapy. Thus, we provide...
Although up to 50% of African green monkeys (AGMs) are infected by simian immunodeficiency viruses (SIV) in their natural habitat, they remain asymptomatic carriers these lentiviruses. They provide an attractive model study not only the origin but also link among genetic variation, host-virus adaptation, and pathogenicity primate SIVagm have been isolated from three species AGM: vervet (Cercopithecus pygerythrus), grivet aethiops), sabaeus sabaeus) monkey. We studied four new isolates a...
Background. Naive T cell recovery is critical for successful immune reconstitution after antiretroviral therapy (ART), but the relative contribution of CD31+ and CD31- naive cells to viral persistence unknown.
Abstract Background CCR5-restricted (R5) human immunodeficiency virus type 1 (HIV-1) variants cause CD4+ T-cell loss in the majority of individuals who progress to AIDS, but mechanisms underlying pathogenicity R5 strains are poorly understood. To better understand envelope glycoprotein (Env) determinants contributing viruses, we characterized 37 full-length Envs from cross-sectional and longitudinal viruses isolated blood patients with asymptomatic infection or referred as pre-AIDS (PA) AIDS...
DNA from the peripheral blood mononuclear cells of 17 different individuals infected with human T-cell lymphoma/leukemia virus type II (HTLV-II) was successfully amplified by polymerase chain reaction (PCR) primer pair SK110/SK111. This is conserved among pol genes all primate lymphoma viruses (PTLV) and flanks a 140-bp fragment which, when used in comparative analyses, reflects relative degree diversity PTLV genomes. Cloning, sequencing, phylogenetic comparisons these fragments indicated...
Recent studies have established the presence of human T-cell lymphoma/leukemia virus type I (HTLV-I) in Melanesia. An HTLV-I strain, PNG-1, has now been isolated from a healthy member Hagahai, remote, recently contacted group Papua New Guinea. To further characterize we employed polymerase chain reaction amplification with subsequent cloning and sequencing amplified products. Sequence analyses regions pol, env, pX genes this variant indicate marked heterogeneity (approximately 7%) prototype...
HIV type 1 viral quasispecies were amplified by polymerase chain reaction (PCR) in the hypervariable V3 region of gp 120 from six different regions brain (right and left frontal; right parietal; occipital) peripheral blood mononuclear cells (PBMCs) a patient who died AIDS dementia complex (ADC). Cloning sequencing entire suggested presence genetically unique sequences brain. In contrast, blood-derived carried homogeneous that characterized single octapeptide crest motif (HLGPGSAF), important...
Elite non-progressors (plasma viral load <50 copies/ml while antiretroviral naive) constitute a tiny fraction of HIV-infected individuals. After 12 years follow-up cohort 13 long-term (LTNP) identified from 135 individuals with transfusion-acquired HIV infection, 5 remained LTNP after 23 to 26 but only 3 retained elite status. We examined the mechanisms that differentiated delayed progressors in this cohort. A survival advantage was conferred on subjects, who had at least one host genetic...
ABSTRACT Most human immunodeficiency virus type 1 (HIV-1) strains isolated from the brain use CCR5 for entry into macrophages and microglia. Strains that both CXCR4 (R5X4 strains) have been identified in brains of some individuals, but mechanisms underlying persistence R5X4 viruses compartmentalized between other tissue reservoirs are unknown. Here, we characterized changes HIV-1 envelope (Env) enhance tropism variants or lymphoid tissue. Envs derived two individuals had enhanced usage...
Abstract Background HIV preferentially infects CD4+ T cells, and the functional impairment numerical decline of CD8+ cells characterize disease. The affects optimal ratio between two cell types necessary for immune regulation. Therefore, this work aimed to define genomic basis interactions with cellular transcriptome both cells. Results Genome-wide transcriptomes primary from HIV+ patients were analyzed at different stages disease using Illumina microarray. For each subset, pairwise...
HIV-1 subtype C (C-HIV) is responsible for most cases worldwide. Although the pathogenesis of C-HIV thought to predominantly involve CCR5-restricted (R5) strains, we do not have a firm understanding how frequently CXCR4-using (X4 and R5X4) variants emerge in subjects with progressive infection. Nor completely understand molecular determinants coreceptor switching by variants. Here, characterized panel envelope glycoproteins (Envs) (n = 300) cloned sequentially from plasma 21 antiretroviral...
Neurodegenerative diseases (NDs) such as Alzheimer's disease (AD), Parkinson's (PD), multiple sclerosis (MS), amyotrophic lateral (ALS), and dementia pose one of the greatest health challenges this century. Although these NDs have been looked at single entities, underlying molecular mechanisms never collectively visualized to date. With advent high-throughput genomic proteomic technologies, we now opportunity visualize in a whole new perspective, which will provide clear understanding...