A5088822328- HIV Research and Treatment
- RNA Research and Splicing
- HIV/AIDS drug development and treatment
- RNA and protein synthesis mechanisms
- HIV/AIDS Research and Interventions
- Ecology and Vegetation Dynamics Studies
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Plant and animal studies
- Cytomegalovirus and herpesvirus research
- Thyroid Disorders and Treatments
- Glycosylation and Glycoproteins Research
- Complement system in diseases
- Genetic diversity and population structure
- RNA Interference and Gene Delivery
- T-cell and Retrovirus Studies
- Herpesvirus Infections and Treatments
- Genomics and Chromatin Dynamics
- T-cell and B-cell Immunology
- Bacteriophages and microbial interactions
- Blood groups and transfusion
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Molecular Biology Techniques and Applications
- Advanced biosensing and bioanalysis techniques
Minneapolis College
2019-2022
University of Minnesota
2010-2019
Monash University
2010-2012
Guanacaste Conservation Area
2011
Burnet Institute
1996-2005
Bridge University
2003
Crowell & Moring (United States)
2003
Fairfield Hospital
1990-2002
AIDS United
2000
Fairfield Infectious Diseases Hospital
1995
A blood donor infected with human immunodeficiency virus-type 1 (HIV-1) and a cohort of six or product recipients from this remain free HIV-1-related disease stable normal CD4 lymphocyte counts 10 to 14 years after infection. HIV-1 sequences either virus isolates patient peripheral mononuclear cells had similar deletions in the nef gene region overlap U3 long terminal repeat (LTR). Full-length sequencing one isolate genome amplification selected regions other members revealed no...
Urbanization transforms environments in ways that alter biological evolution. We examined whether urban environmental change drives parallel evolution by sampling 110,019 white clover plants from 6169 populations 160 cities globally. Plants were assayed for a Mendelian antiherbivore defense also affects tolerance to abiotic stressors. Urban-rural gradients associated with the of clines 47% throughout world. Variation strength was explained changes drought stress and vegetation cover varied...
The Sydney Blood Bank Cohort consists of a blood donor and eight transfusion recipients who were infected before 1985 with strain human immunodeficiency virus type 1 (HIV-1) deletion in the region which nef gene long terminal repeat overlap. Two have died since 1994, at 77 83 years age, causes unrelated to HIV infection; one other recipient, had systemic lupus erythematosus, 1987 22 age possibly related HIV. We present longitudinal immunologic virologic data on six surviving members deceased...
Peripheral blood monocytes are resistant to productive human immunodeficiency virus type 1 (HIV-1) infection in vitro immediately after isolation. No viral cDNA (either early or late transcripts) was detected by PCR exposed on the day of In contrast, cultured for as little day, initiated and completed reverse transcripts were readily detectable within 24 h with both HIV-1(Ba-L) primary isolates. The levels initiated, partially completed, DNA copies found increased progressively time culture...
Aim We investigated the phylogeography, geographical variation in leaf morphology, freezing tolerance and climatic niches of two widespread evergreen sister oak species (Quercus) series Virentes. Location South-eastern USA, Mexico Central America. Methods Nuclear microsatellites non-recombining nuclear chloroplast DNA sequences were obtained from trees throughout range lineages live oaks, represented by Quercus virginiana temperate zone Q. oleoides tropics. Divergence times estimated for...
We have attempted to ascertain the correlation between genetic information content in exons and surrounding intron sequences with regard their spatial arrangement within a gene. A comparison is made of sizes, taken from recent publications, introns approximately equal 80 different protein-coding chromosomal genes, mostly higher eukaryotes. The these genes do not show very marked variation size can be classified into three major discrete two minor additional groups, whereas individual vary...
ABSTRACT Long-term survivors (LTS) of human immunodeficiency virus type 1 (HIV-1) infection provide an opportunity to investigate both viral and host factors that influence the rate disease progression. We have identified three HIV-1-infected individuals in Australia who been infected for over 11 years with viruses contain deletions nef -long terminal repeat ( /LTR) overlap regions. These differ from each other -defective strains HIV-1 previously Australia. One individual, LTS 3, is a...
Sequencing of the reverse transcriptase (RT) region 26 human immunodeficiency virus type 1 (HIV-1) isolates from eight patients treated with 3'-azido-3'-deoxythymidine (AZT) revealed a mutation at codon 210 TTG (leucine) to TGG (tryptophan) exclusively in association resistance AZT. The Trp-210 was observed 15 20 phenotypically resistant AZT, being more commonly than resistance-associated mutations codons 67, 70, and 219. never before emergence Leu-41 Tyr-215, sequential series five one...
Aim To determine why some communities are more invasible than others and how this depends on spatial scale. Our previous work in serpentine ecosystems showed that native exotic diversity negatively correlated at small scales, but became positively larger scales. We hypothesized pattern was the result of classic niche partitioning scales where environment is homogeneous, a shift to dominance coexistence mechanisms depend heterogeneity large Location Serpentine ecosystem, Northern California....
Abstract The impacts of drought are expanding worldwide as a consequence climate change. However, there is still little knowledge how species respond to long‐term selection in seasonally dry ecosystems. In this study, we used Q ST ‐ F comparisons investigate (i) the role natural on population genetic differentiation for set functional traits related resistance tropical oak Quercus oleoides and (ii) influence water availability at site origin experimental treatments patterns trait divergence....
The murine Fc receptor for IgG (Fc gamma R) was purified to homogeneity by immunoaffinity chromatography from detergent lysates of the macrophage cell line J774. Microsequencing intact protein yielded a single amino-terminal sequence, which confirmed and extended 20 residues isolation an overlapping peptide. additional proteolytic fragments obtained using Staphylococcus aureus V8 protease, cyanogen bromide, lysine C proteinase, facilitated sequence analysis total 119 amino acid residues....
The 3′-terminal sequences of 18S rRNA from chicken reticulocyte, mouse sarcoma, rat liver, rabbit reticulocyte and barley embryo were determined by the direct chemical sequencing method. regions sequenced show complete homology for first 73 nucleotides. A sequence 5′-proximal to m 26 Am residues that is complementary eukaryotic 5S RNAs totally conserved. This supports hypothesis base-paired interaction between rRNA, which are present in large small ribosomal subunits respectively, may be...
We studied the evolution of human immunodeficiency virus type 1 (HIV-1) in a cohort long-term survivors infected with an attenuated strain HIV-1 acquired from single source. Although members experienced differing clinical courses, we demonstrate similar nef/long-terminal repeat (LTR) sequences, characterized by progressive sequence deletions tending toward minimal nef/LTR structure that retains only elements required for viral replication. The vivo pathogenicity is therefore dictated and/or...
Quercus oleoides Cham. and Schlect., tropical live oak, is a species of conservation importance in its southern range limit northwestern Costa Rica. It occurs high-density stands across fragmented landscape spanning contrasting elevation precipitation gradient. We examined genetic diversity spatial structure this geographically isolated genetically distinct population. characterized population at 11 nuclear microsatellite loci 260 individuals from 13 sites. monitored flowering time 10 sites,...
ABSTRACT An explosive epidemic of human immunodeficiency virus type 1 (HIV-1) has been documented among the injecting drug user population Kathmandu, Nepal, whose seropositivity rate risen from 0 to 40% between 1995 and 1997. By using Catrimox preserve whole-blood RNA at ambient temperature for transportation, HIV-1 envelope V3–V4 sequences were obtained 36 patients in this group. Analysis indicated a homogenous subtype C virus, with least two independent introductions into population. Viral...
Journal Article Complete nucleotide sequence of a cloned chicken a-globin cDNA Get access Nicholas J. Deacon, Deacon Molecular Biology Unit, Research School Biological Sciences, The Australian National UniversityP.O. Box 475, Canberra City, A.C.T. 2601, Australia Search for other works by this author on: Oxford Academic PubMed Google Scholar John Shine, Shine Hiroto Naora Nucleic Acids Research, Volume 8, Issue 6, 25 March 1980, Pages 1187–1200, https://doi.org/10.1093/nar/8.6.1187...
PREMISE OF THE STUDY: Reproductive phenology is important for tree species that occur in seasonally dry environments, particularly those with desiccation‐sensitive, nondormant seeds. In this study, we compared germination, growth, and survival of seeds the evergreen tropical live oak Quercus oleoides produced at different times during wet season two sites differ rainfall along an elevation gradient. Our goal was to determine effects reproductive timing on germination juvenile fitness...