A5072903360- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- interferon and immune responses
- Immune Cell Function and Interaction
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Systemic Lupus Erythematosus Research
- HIV/AIDS Research and Interventions
- Herpesvirus Infections and Treatments
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- RNA Research and Splicing
- Immune Response and Inflammation
- Food Allergy and Anaphylaxis Research
- Cytomegalovirus and herpesvirus research
- Antimicrobial Peptides and Activities
- Respiratory viral infections research
- Participatory Visual Research Methods
- Disaster Management and Resilience
- vaccines and immunoinformatics approaches
- Cancer-related Molecular Pathways
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV-related health complications and treatments
Burnet Institute
1998-2013
Fairfield Hospital
1994-2002
AIDS United
2001-2002
Monash Medical Centre
1995
Monash University
1992-1994
Commonwealth Scientific and Industrial Research Organisation
1994
A blood donor infected with human immunodeficiency virus-type 1 (HIV-1) and a cohort of six or product recipients from this remain free HIV-1-related disease stable normal CD4 lymphocyte counts 10 to 14 years after infection. HIV-1 sequences either virus isolates patient peripheral mononuclear cells had similar deletions in the nef gene region overlap U3 long terminal repeat (LTR). Full-length sequencing one isolate genome amplification selected regions other members revealed no...
ABSTRACT The nef gene product of human immunodeficiency virus type 1 (HIV-1) is important for the induction AIDS, and key to its function ability manipulate T-cell by targeting cellular signal transduction proteins. We reported that Nef coprecipitates a multiprotein complex from cells which contains tumor suppressor protein p53. now show interacts directly with Binding assays showed an N-terminal, 57-residue fragment (Nef 1-57) p53-binding domain. also interacted p53 during HIV-1 infection...
It is now well established that human immunodeficiency virus type I (HIV-1) Nef contributes substantially to disease pathogenesis by augmenting replication and markedly perturbing T-cell function. The effect of on host cell activation could be explained in part its interaction with specific cellular proteins involved signal transduction, including at least a member the src family kinase, Lck, serine/threonine mitogen-activated protein kinase (MAPK). Recombinant directly interacted purified...
Human immunodeficiency virus type 1 (HIV-1) Nef protein causes the loss of cell surface CD4 and interleukin-2 (IL-2) receptor (Tac) from peripheral blood mononuclear cells (PBMC) CD4+ T-cell lines. As both IL-2 play crucial roles in antigen-driven helper signalling proliferation, respectively, role viral life cycle may be to perturb pathways emanating these receptors. However, intracellular targets for that result down-regulation are unknown. Using a recombinant glutathione...
ABSTRACT The nef gene from human and simian immunodeficiency viruses (HIV SIV) regulates cell function viral replication, possibly through binding of the product to cellular proteins, including Src family tyrosine kinases. We show here that Nef protein encoded by SIVmac239 interacts with also activates kinases Lck Hck. This is in direct contrast inhibitory effect HIV type 1 (HIV-1) on catalytic activity. Unexpectedly, however, interaction SIV or Hck not mediated via its consensus proline...
Objective: To investigate whether members of a transfusion-linked cohort (the Sydney Bloodbank Cohort) infected with nef-deleted strain HIV-1 could be differentiated from individuals wild-type strains by characterizing the Nef antibody response members. Design: Retrospective and prospective analysis nef gene sequence to peptides in HIV-infected subjects. Methods: Plasma was obtained all cohort, variety HIV-1-infected uninfected controls. Antibodies recognizing full-length recombinant...
Objective HIV-1 infection impairs a number of macrophage effector functions, but the mechanism is unknown. We studied role Nef in modulating phagocytosis by human monocytes and monocyte-derived macrophages (MDM). Design methods Using flow cytometric assay, Mycobacterium avium complex (MAC) whole blood Sydney Blood Bank Cohort (SBBC) members infected with nef-deleted (Δnef) strain was compared that from uninfected or wild-type (WT) HIV-infected subjects. The specific impact on MDM determined...
The proline repeat motif (PxxP) of Nef is required for interaction with the SH3 domains macrophage-specific Src kinase Hck. However, implication this viral replication and infectivity in macrophages T lymphocytes remains unclear. Experiments HIV-1 infected confirmed presence a Nef:Hck complex which was dependent on motif. also enhanced both infection macrophages, incorporation Hck into particles. Unexpectedly, wild-type inhibited but shown to enhance primary lymphocytes. These results...
The constitutive production of interferon-alpha (IFN-alpha) subtypes by the lymphoblastoid cell lines, Namalwa, Daudi and Raji, was investigated using sensitive semi-quantitative flow cytometric techniques. Further, we sought to determine whether previously described failure these lines produce IFN-alpha-4 a result deletion IFN A4 gene. Cytoplasmic IFN-alpha-2 assessed IFN-alpha subtype-specific antipeptide antibodies FITC-labelled secondary in indirect immunofluorescence-flow cytometry...
Summary The purpose of this study was to produce antibodies which could be used investigate the expression murine (Mu)IFN‐α. Rabbits were immunized with a peptide, corresponding 15 COOH‐terminal amino acids MuIFN‐α‐1, conjugated keyhole limpet haemocyanin (KLH), and resulting antipeptide identified by indirect ELISA. Antipeptide purified from rabbit immune sera immunoadsorption peptide immobilized on nitrocellulose any remaining KLH removed KLH‐Sepharose. characterization ELISA,...
This paper reflects on the place of ethnography in collaborative learning context urban environmental management. The Low Impact Urban Design and Development (LIUDD) research programme is aimed at facilitating uptake implementation low-impact principles, with a focus improved approaches to storm-water Collaborative include use case studies, groups web-based database. Place-based studies are intended showcase some examples LIUDD develop forums for refining questions developing innovative...