A5061125373- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- RNA Interference and Gene Delivery
- HIV/AIDS Research and Interventions
- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Cell death mechanisms and regulation
- Malaria Research and Control
- Erythrocyte Function and Pathophysiology
- Adenosine and Purinergic Signaling
- RNA and protein synthesis mechanisms
- Hemoglobinopathies and Related Disorders
- Hepatitis C virus research
- Textile materials and evaluations
- Complement system in diseases
- T-cell and B-cell Immunology
- Insect-Plant Interactions and Control
- Blood properties and coagulation
- Infectious Encephalopathies and Encephalitis
- Polymer crystallization and properties
- Advanced biosensing and bioanalysis techniques
- Biochemical and Molecular Research
- Research on Leishmaniasis Studies
- Dendrimers and Hyperbranched Polymers
- Nuclear Structure and Function
Monash University
2010-2021
Vector & Vector-Borne Diseases Research Institute
2014-2015
The University of Melbourne
1985-2014
Burnet Institute
2008-2013
The University of Western Australia
2010
St Vincent's Hospital Sydney
2010
Fairfield Hospital
1995-1996
Victorian Infectious Diseases Reference Laboratory
1996
University of Pittsburgh Medical Center
1995
Fairfield Infectious Diseases Hospital
1995
A blood donor infected with human immunodeficiency virus-type 1 (HIV-1) and a cohort of six or product recipients from this remain free HIV-1-related disease stable normal CD4 lymphocyte counts 10 to 14 years after infection. HIV-1 sequences either virus isolates patient peripheral mononuclear cells had similar deletions in the nef gene region overlap U3 long terminal repeat (LTR). Full-length sequencing one isolate genome amplification selected regions other members revealed no...
Sequencing of the reverse transcriptase (RT) region 26 human immunodeficiency virus type 1 (HIV-1) isolates from eight patients treated with 3'-azido-3'-deoxythymidine (AZT) revealed a mutation at codon 210 TTG (leucine) to TGG (tryptophan) exclusively in association resistance AZT. The Trp-210 was observed 15 20 phenotypically resistant AZT, being more commonly than resistance-associated mutations codons 67, 70, and 219. never before emergence Leu-41 Tyr-215, sequential series five one...
The World Mosquito Program uses Wolbachia pipientis for the biocontrol of arboviruses transmitted by Aedes aegypti mosquitoes. Diagnostic testing in laboratory colonies and field-caught mosquito populations has typically employed PCR. New, simpler methods to diagnose infection mosquitoes are required large-scale operational use. Field-collected Ae. from North Queensland were tested using primers designed detect wsp gene, specific strain wMel. results analysed colour change reaction mix....
Several critical events of apoptosis occur in the cell nucleus, including inter-nucleosomal DNA fragmentation (apoptotic DNA) and eventual chromatin condensation. The generation apoptotic has become a biochemical hallmark because it is late 'point no return' step both extrinsic (cell-death receptor) intrinsic (mitochondrial) pathways. Despite investigators observing understanding its decisive role as marker for over 20 years, measuring proved elusive. We have integrated ligation-mediated PCR...
Background Biological control programs involving Wolbachia -infected Aedes aegypti are currently deployed in different epidemiological settings. New Caledonia (NC) is an ideal location for the implementation and evaluation of such a strategy as only proven vector dengue virus (DENV) Ae . outbreaks frequency severity increasing. We report generation NC Wolbachia- infected strain results experiments to assess competence fitness this future disease Noumea, NC. Methods/principal findings The...
Sensory neuropathy (SN) is common in patients with HIV. Hepatitis C (HCV) coinfection often cited as an HIV-SN risk factor, but data to support this are lacking. This collaboration aimed examine the association between HCV serostatus and SN among ambulatory HIV-positive patients.Patients HIV were assessed cross-sectional studies Baltimore, Jakarta, Johannesburg, Kuala Lumpur, Melbourne, Sydney for (defined by both supportive symptoms signs). seropositivity was using a chi(2) test, followed...
Parasitic protozoa, such as Leishmania species, are thought to express a number of surface and secreted nucleoside triphosphate diphosphohydrolases (NTPDases) which hydrolyze broad range tri- diphosphates. However, the functional significance NTPDases in parasite virulence is poorly defined. The major genome was found contain two putative NTPDases, termed LmNTPDase1 2, with predicted NTPDase catalytic domains either an N-terminal signal sequence and/or transmembrane domain, respectively....
Abstract Sequential human immunodeficiency virus type 1 (HIV‐1) isolates were obtained over a 29‐month period from person before, during, and after AZT therapy. DNA sequence analysis of polymerase chain‐amplified reverse‐transcriptase gene showed gradual accumulation mutations to peak resistance (lC 50 2.13 μM AZT) in association with at codons 44, 210, 369, as well 41, 67, 70, 215. Eight months cessation therapy, when an HIV‐1 isolate the patient was again sensitive AZT, these had all...
Apoptosis has a critical role in normal physiology while its dysregulation causal links with certain pathologies. A biochemical hallmark of apoptosis, internucleosomal genomic DNA fragmentation, is detectable by ligation-mediated polymerase chain reaction (LM-PCR). Here we converted LM-PCR into new apoptosis quantifier dividing trace quantities 600 bp apoptotic amplicons those single copy house-keeping gene, generating the 'value'. Dynamic range was approximately 17-fold correlating 200-fold...
To establish the syncytium-inducing (SI) phenotype and zidovudine (ZDV) susceptibility of HIV-1 isolates obtained from autopsy specimens.Isolation HIV was attempted specimens 76 AIDS patients. Specimens lymph node, spleen, spinal cord, brain cerebrospinal fluid (CSF) were processed cultured with peripheral blood mononuclear cells (PBMC) seronegative donors. Biological determined in a T-lymphocyte line (MT-2). ZDV evaluated PBMC-based assay. Sequencing amino-acid codons reverse transcriptase...
Background: Peripheral neuropathy is the dose-limiting toxicity of stavudine and didanosine (nucleoside analogs used in HIV treatment) attributed to mitochondrial from these drugs. Acetyl L-carnitine (ALC) co-enzyme Q10 are proposed as treatments, but evidence support limited. Methods: We examined ALC a water-soluble formulation (HQO™) for prevention d4T ddI neurotoxicity using cultured fetal rat DRG an vitro model. Results: DdI (33μM) (50μM) caused clear (impaired neurite growth) by day 8...
The nely orchestrated cell death process of apoptosis is fundamental to vertebrate and invertebrate health growth, while its dysregulation contributes infectious disease pathology malignancy.Additionally, it vital evaluate the effects on drug candidates for cancer therapy.In view important role plays in health, disease, design, therapy conservation across species, therefore highly bene cial be able measure apoptosis.In this article we describe ApoqPCR, a protocol by absolute quantitation an...