A5076676217- MicroRNA in disease regulation
- Chromatin Remodeling and Cancer
- Cancer Mechanisms and Therapy
- Lymphoma Diagnosis and Treatment
- Neuroendocrine Tumor Research Advances
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Hepatitis C virus research
- Immune Cell Function and Interaction
- Helicobacter pylori-related gastroenterology studies
- Viral-associated cancers and disorders
- Hematopoietic Stem Cell Transplantation
- Colorectal Cancer Treatments and Studies
- Eosinophilic Disorders and Syndromes
- Hepatitis B Virus Studies
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Chronic Myeloid Leukemia Treatments
- Sarcoma Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Gastrointestinal disorders and treatments
- Genetic factors in colorectal cancer
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Neuroblastoma Research and Treatments
- Gastrointestinal Tumor Research and Treatment
Hakodate National Hospital
2015-2024
Fujita Health University
2010-2023
Yokkaichi Nursing and Medical Care University
2021-2023
Shimada Municipal Hospital
2019-2022
Kurume University
2021
Komatsu (Japan)
2020
Nagoya University
2017-2019
Nagoya City University
2019
Fujita Health University Hospital
2012-2018
National Center For Child Health and Development
2018
MicroRNAs (miRNAs) are 21 to approximately 25-nucleotides (nt) long and interact with mRNAs trigger either translational repression or RNA cleavage through interference (RNAi), depending on the degree of complementarity target mRNAs. Our recent study has shown that HIV-1 nef dsRNA from AIDS patients who long-term non-progressors (LTNPs) inhibited transcription HIV-1.Here, we show possibility nef-derived miRNAs produced in persistently infected cells. Furthermore, short hairpin (shRNA)...
Abstract Purpose: To better understand microRNA miR-21 function in carcinogenesis, we analyzed expression patterns different stages of colorectal cancer development using situ hybridization (ISH). Experimental Design: Locked nucleic acid (LNA)/DNA probes and a biotin-free tyramide signal amplification system were used ISH analyses miRNA expression. Conditions for specific detection determined human cell lines miR-21–expressing lentiviral vectors. Expression was 39 surgically excised tumors...
Apoptotic cells were histochemically demonstrated by the TdT-mediated biotinylated dUTP nick end-labeling (TUNEL) method in formalin-fixed and paraffin-embedded sections of human endometrium placental villi. In 53 endometrial biopsy specimens, labeled nuclei identified 16 samples showing a desquamating change, associated with menstruation, functional bleeding or adenocarcinoma. Cells normal proliferative secretory phases unlabeled. The gland stroma corresponded well to so-called apoptotic...
The chromatin remodeling complex SWI/SNF is an important epigenetic regulator that includes one Brm or BRG1 molecule as catalytic subunit. and do not function identically, so this can regulate gene expression either positively negatively, depending on the promoter to which it recruited. Notably, attenuation due posttranscription suppression occurs often in human tumor cells, event contributes their oncogenic potential. Here, we report 3'-untranslated region of mRNA has two sites are...
Apocrine carcinoma, a subtype of invasive ductal carcinoma the breast, expresses androgen receptor (AR), but often lacks estrogen (ER) and progesterone (PgR). In present study, author immunohistochemically defined apocrine-type as ER-/PgR-/AR+ analyzed significance triple-negative breast cancer.Four hundred forty cancers from 429 cases were immunostained for receptor, human epidermal growth factor type 2 (HER2), p53, Ki-67 receptor. The lesions included 58 in situ malignancies (including 13...
Abstract Exosomes, a type of small extracellular vesicles (sEVs), derived from multivesicular bodies (MVBs), mediate cell-to-cell communication by transporting proteins, mRNAs, and miRNAs. However, the molecular mechanism which proteins are sorted to sEVs is not fully understood. Here, we report that ubiquitin-like 3 (UBL3)/membrane-anchored Ub-fold protein (MUB) acts as posttranslational modification (PTM) factor regulates sorting sEVs. We find UBL3 indispensable for MVBs also observe 60%...
Immunohistochemical and clinicopathological features of 58 gastrointestinal stromal tumors (GIST) were studied. One occurred in the esophagus, 41 stomach, nine small intestine, seven large intestine. By using indirect immunoperoxidase staining for Cajal cell markers (c-kit protein CD34), smooth muscle (alpha-smooth actin, desmin, heavy caldesmon calponin) Schwann (S-100 Leu 7), GIST classified into five groups, such as type (n = 9), myogenic 5), 2), mixed 40) undifferentiated 2). c-kit...
The mammalian SWI/SNF chromatin remodeling complex, an essential epigenetic regulator, contains either a single Brm or BRG1 molecule as its catalytic subunit. We observed frequent loss of expression but not in human gastric cancer cell lines. Treatment with histone deacetylase inhibitor rescued expression, indicating regulation this gene, and RNA interference-based colony formation assay revealed antioncogenic properties Brm. immunostaining 89 primary cancers showed obvious reduction 60...
Gastric cancer (GC) presents various histological features, though the mechanism underlying its diversity is seldom elucidated. It mainly classified into well differentiated tubular adenocarcinoma (tub1), moderately (tub2), poorly (por), signet-ring cell carcinoma (sig), mucinous (muc), and papillary (pap). By screening, we found cathepsin E (CTSE) expresses universally in sig-type, occasionally por-type, rarely tub1/tub2-type GC lines. In surgically-resected specimens, CTSE was...
A patient with Non-Hodgkin's lymphoma is reported, in which reactivation of the hepatitis B virus was achieved from treatment rituximab. The patient's HBs antigens were positive on admission, and she tested for HBs, HBe, HBc antibodies, negative HBe antigens. She treated a regimen three courses rituximab-containing anti-cancer drugs one course combined drugs. Throughout these chemotherapy courses, prednisolone not given. After fourth third rituximab developed hepatic dysfunction, serum...
Degenerative processes of elastic fibers in sun-protected and sun-exposed skin were analyzed by light electron microscopic (post-embedding) immunocytochemistry using antisera to elastin, fibrillin-1, amyloid P component, lysozyme alpha1-antitrypsin. To assess the effect aging sun exposure, biopsy specimens (back) severely moderately (face forearms) obtained from a young age group (1-27 years), an adult (31-56 years) old aged (61-100 years). Elastin fibrillin-1 essential components fibers;...
Immunohistochemical demonstration of proliferating cell nuclear antigen (PCNA) and p53 protein is important, particularly for the surgical diagnosis neoplastic disorders. An effective, simple reproducible method was established observing expression these intranuclear antigens in routinely processed, formalin-fixed paraffin-embedded sections. Dramatic improvement antigenicity obtained when deparaffinized sections were heated a hot water bath at 90 degrees C 120 min 0.01 mol/L citrate buffer,...
✓ An easy, accurate, and safe biopsy technique for deep-seated brain tumors using a newly devised real-time ultrasonic apparatus is presented. Three lesions, one large pituitary adenoma two pineal region tumors, were biopsied successfully by this technique.
The development of a consistent strategy for the analysis oncogene expression at cellular level is essential understanding roles these genes in and progression human neoplasia. Detection neu products breast carcinoma was selected as model expression. Fifty-two primary carcinomas were evaluated by quantitation DNA amplification mRNA localization protein (pi 85) situ hybridization (ISH) immunohistochemistry (IHC). specificity sensitivity molecular immunologic probes established with use...
Carcinoembryonic antigen (CEA) and its related substances in normal neoplastic pancreas were studied by the indirect immunoperoxidase method, using six conventional monoclonal antibodies. Gastric colonic tissues used as positive controls. The antigenicity of CEA CEA-related more unstable than expected. Normal gastric epithelial cells unequivocally for antigens frozen sections, but their staining intensity weakened paraffin sections. In pancreatic ducts, there was nonspecific cross-reacting...
Carcinoembryonic antigen (CEA) and secretory component (SC) were localized by peroxidase-labeled antibody immunocytochemistry in normal abnormal human gastric mucosa. In epithelium, both glycoproteins absent or only faintly present, but intestinal metaplasia carcinoma prominently present. CEA SC on the surfaces of metaplastic epithelial cells polarized. That is, was expressed microvillous surface basolateral surface. cancer, distributed over entire neoplastic cells. Thus, deviations from...