We investigated the effect of fibronectin on epithelial migration onto stroma in cultured rabbit cornea. Rabbit plasma was purified by affinity chromatography using gelatin-Sepharose 4B, and its purity confirmed SDS polyacrylamide slab gel electrophoresis. Antibody against raised guinea pigs formed a single precipitin line Ouchterlony double diffusion test. When cornea cut into small blocks TCM-199 medium alone, corneal cells began to migrate edge stroma. The addition culture significantly...

Using peroxidase-antiperoxidase (PAP) method, carcinoembryonic antigen (CEA) was demonstrated in conventionally processed colorectal cancer tissues.A new immunohistochemical grading for cancers based on the mode of localization made an attempt to clarify factors responsible elevation plasma CEA levels patients.Most patients with well differentiated adenocarcinoma, which densely distributed along apical surface but only rarely present basolateral surfaces carcinoma cells, had very low CEA,...

The beneficial effects of endurance exercise include insulin-sensitization and reduction fat mass. Limited knowledge is available about the mechanisms by which exerts salutary effects. Myokines, cytokines secreted skeletal muscle, have been recognized as a potential mediator. Recently, role muscle-derived interleukin-15 (IL-15) in improvement fat-lean body mass composition insulin sensitivity has proposed. Yet, previous studies reported that training does not increase production or secretion...

The intestine-specific inhibitor of alkaline phosphatase, l-phenylalanine, has been employed in the study phosphatase rat intestine. Five substrates were used, β-glycerophosphate, o-carboxyphenylphosphate, α-naphthyl acid phosphate, naphthol AS-BI phosphate and AS-TR phosphate. hydrolytic rate specific l-phenylalanine inhibition was most marked case first three substrates. When formol-Ca fixed, gum-sucrose treated sections intestine incubated these presence separately 0.05 Md- Gomori's...

Some of the lysozyme mutants in humans cause systemic amyloidosis.Hen egg white (HEWL) has been well studied as a model protein amyloid fibrils formation.We previously identified an core region consisting nine amino acids (designated K peptide), which is present at 54-62 HEWL.The peptide, with tryptophan its C-terminus, ability self-aggregation.In work we focused on structural properties relation to formation fibrils.The peptide alone formed definite having β-sheet structures by incubation 7...

A bstract Metastable cosmic strings appear in models of new physics with a two-step symmetry breaking G → H 1, where π 1 ( ) ≠ 0 and = 0. They decay via the monopole-antimonopole pair creation inside. Conventionally, rate has been estimated by an infinitely thin string approximation, which requires large hierarchy between scales. In this paper, we reexamine it taking into account finite sizes both monopole. We obtain robust lower limit on tunneling factor $$ {e}^{-{S}_B} <mml:math...

Novel antimicrobial peptides (AMP), designated Fa-AMP1 and Fa-AMP2, were purified from the seeds of buckwheat (Fagopyrum esculentum Moench.) by gel filtration on Sephadex G75, ion-exchange HPLC SP COSMOGEL, reverse-phase HPLC. They basic having isoelectric points over 10. Fa-AMP2 had molecular masses 3,879 Da 3,906 MALDI-TOF MS analysis, their extinction coefficients in 1% aqueous solutions at 280 nm 42.8 38.9, respectively. Half all amino acid residues cysteine glycine, they continuous...

Polypoid carcinomas with spindle-cell sarcomatous features have been designated either as carcinosarcoma or pseudosarcoma. The distinction between these two tumors has depended on the presence of "intermingling" carcinomatous and components in so-called carcinosarcoma. But unlike carcinosarcoma, component pseudosarcoma considered benign. A polypoid tumor esophagus a 57-year-old male was predominantly composed spindle cell, cells. adjacent intraepithelial epidermoid carcinoma transitional...

Abstract To obtain a broad perspective of the events leading to spontaneous loss heterozygosity (LOH), we have characterized genetic alterations that functionally inactivated URA3 marker hemizygously or heterozygously situated either on chromosome III V in diploid Saccharomyces cerevisiae cells. Analysis structure large number LOH clones by pulsed-field gel electrophoresis and PCR showed loss, allelic recombination, aberration were major classes LOH. The frequencies significantly affected...

To assess the role of Cu-Zn superoxide dismutase (CuZnSOD) in regulating cellular antioxidant defenses, we studied induction CuZnSOD mRNA by an situ hybridization technique and protein immunocytochemical method gerbil hippocampus following 5 min transient global ischemia. For hybridization, synthesized 48-mer oligonucleotide (base 465–512) complementary to rat mRNA. Northern blot analysis showed a single band molecular weight 0.65 kb. After ischemia, signal became stronger at 3 24 h returned...

Two novel chitin-binding peptides, designated Pp-AMP 1 and 2, which had antimicrobial activity against pathogenic bacteria fungi, were purified from Japanese bamboo shoots (Phyllostachys pubescens) by a simple procedure based on chitin affinity chromatography. They the common structural features of plant defensin family, but they could not be grouped in any type that family. showed high degree homology to mistletoe toxins.

Abstract Intrathyroidal thymoma is a rare tumor that occurs in the thyroid gland and has been reported as low‐grade malignant tumor. The present report describes 59‐year‐old male patient with this tumor, who was treated subtotal thyroidectomy followed by radiation chemotherapy. located lower pole of left lobe isthmus thyroid, it had invaded parenchyma, capsule, adjacent connective tissue, neck muscles. Continuity between cervical thymus noted. Subsequently, developed widespread metastases...

Monoclonal antibodies were raised against arachidonate 12-lipoxygenase using a partially purified enzyme from porcine leukocytes as an antigen.Immunohistochemical studies indicated selective localization of in the cytosol polymorphonuclear leukocytes.Two separate species antibody (lox-1 and lox-2) recognizing different sites protein utilized to develop method determine amount rather than activity enzyme.Fab fragment lox-2 was conjugated horseradish peroxidase, conjugate label bound...

Carcinoembryonic antigen (CEA) and its related substances in normal neoplastic pancreas were studied by the indirect immunoperoxidase method, using six conventional monoclonal antibodies. Gastric colonic tissues used as positive controls. The antigenicity of CEA CEA-related more unstable than expected. Normal gastric epithelial cells unequivocally for antigens frozen sections, but their staining intensity weakened paraffin sections. In pancreatic ducts, there was nonspecific cross-reacting...

Carcinoembryonic antigen (CEA) and secretory component (SC) were localized by peroxidase-labeled antibody immunocytochemistry in normal abnormal human gastric mucosa. In epithelium, both glycoproteins absent or only faintly present, but intestinal metaplasia carcinoma prominently present. CEA SC on the surfaces of metaplastic epithelial cells polarized. That is, was expressed microvillous surface basolateral surface. cancer, distributed over entire neoplastic cells. Thus, deviations from...

A bstract This paper explores nucleon decay within the framework of a “fake Grand Unified Theory (GUT)” combined with Froggatt-Nielsen (FN) mechanism. In this fake GUT framework, quarks and leptons may have distinct high-energy origins but fit into complete SU(5) multiplets at low energies without requiring force unification, setting it apart from conventional GUTs. By introducing flavor symmetry through FN mechanism, model addresses puzzle quark lepton mass hierarchies mixing patterns. Our...

Novel antimicrobial peptides (AMP), designated Tu-AMP 1 and 2, were purified from the bulbs of tulip (Tulipa gesneriana L.) by chitin affinity chromatography reverse-phase high-performance liquid (HPLC). They bind to in a reversible way. basic having isoelectric points over 12. 2 had molecular masses 4,988 Da 5,006 on MALDI-TOF MS analysis, their extinction coefficients 1% aqueous solutions at 280 nm 3.3 3.4, respectively. Half all amino acid residues occupied cysteine, arginine, lysine,...