A5049841678- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Plant Molecular Biology Research
- Neurobiology and Insect Physiology Research
- Cancer-related gene regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Insect Resistance and Genetics
- Ubiquitin and proteasome pathways
- Animal Behavior and Reproduction
- Animal Genetics and Reproduction
- Protist diversity and phylogeny
- Invertebrate Immune Response Mechanisms
- Histone Deacetylase Inhibitors Research
- Cancer-related molecular mechanisms research
- Genomics and Phylogenetic Studies
- Physiological and biochemical adaptations
- RNA modifications and cancer
- Cholinesterase and Neurodegenerative Diseases
- Heat shock proteins research
- Genetic diversity and population structure
- Computational Drug Discovery Methods
- Plant Reproductive Biology
University of Geneva
2011-2024
Harvard University
1983-2007
Erasmus MC
2005
Princeton University
1993
Hungarian Academy of Sciences
1990-1993
Germ-line transformation via transposable elements is a powerful tool to study gene function in Drosophila melanogaster. However, some inherent characteristics of transposon-mediated transgenesis limit its use for transgene analysis. Here, we circumvent these limitations by optimizing phiC31-based integration system. We generated collection lines with precisely mapped attP sites that allow the insertion transgenes into many different predetermined intergenic locations throughout fly genome....
The bithorax complex in Drosophila melanogaster is a cluster of homeotic genes that specify developmental pathways for many the body segments fly. DNA has been isolated, and region 195,000 base pairs covers left half described here. lesions associated with mutants have identified, most are due to rearrangements. Most spontaneous insertions particular mobile element named "gypsy." This affects functions sequences removed from site insertion. Mutant given phenotypic class distributed over...
The abdominal A (abdA) gene is one of three transcription units in the Bithorax Complex Drosophila encoding a homeo box protein; it flanked by Ultrabithorax (Ubx) and Abdominal B (AbdB). abdA required for segmental identity second through eighth segments. unit approximately 20 kb long encodes protein 330 amino acids. almost identical to Ubx but quite different from AbdB box. polyclonal antibody stains embryonic nuclei segments A1-A7 (parasegments 7-13). iab-2, 3, 4 mutant classes define...
Quantitative and qualitative changes in acetylcholinesterase confer resistance to insecticides.We have constructed several Drosophila melanogaster strains producing various amounts of enzyme by P-mediated transformation.Toxicological analysis these demonstrates that organophosphorus insecticides is correlated with the amount central nervous system.Resistance may also be qualitatively determined.Comparison gene between a resistant strain caught wild type susceptible only revealed one...
The Drosophila bithorax complex Abdominal-B (Abd-B) gene specifies parasegmental identity at the posterior end of fly. specific pattern Abd-B expression in each parasegment (PS) determines its and, PS10-13, is controlled by four parasegment-specific cis-regulatory domains, iab-5 to iab-8, respectively. In order properly determine identity, these domains must function autonomously during both initiation and maintenance phases BX-C regulation. studies reported here demonstrate that...
In the work reported here we have undertaken a functional dissection of Polycomb response element (PRE) from iab-7 cis-regulatory domain Drosophila melanogasterbithorax complex (BX-C). Previous studies mapped iab-7PRE to an 860-bp fragment located just distal Fab-7boundary. Located within this is ∼230-bp chromatin-specific nuclease-hypersensitive region called HS3. We shown that HS3 capable functioning as Polycomb-dependent silencer in vivo, inducing pairing-dependent silencing amini-white...
A very large cis-regulatory region of approximately 300 kb is responsible for the complex patterns expression three homeotic genes bithorax Ubx, abd-A and Abd-B. This can be subdivided in nine parasegment-specific regulatory subunits. Recent genetic molecular analysis has revealed existence two novel elements Mcp Fab-7. located between iab-4 iab-5, subunits which direct Abd-B parasegments 9 10. Similarly, Fab-7 iab-6 iab-7, parasegment 11 12-specific units. appear to function as domain...
ABSTRACT The GAGA protein of Drosophila was first identified as a stimulatory factor in vitro transcription assays using the engrailed and Ultrabithorax promoters. Subsequent studies have suggested that promotes by blocking repressive effects histones; moreover, it has been shown to function chromatin remodeling, acting together with other factors formation nuclease hypersensitive sites vitro. is encoded Trithorax-like locus reported here we used maternal effect allele Trl13C examine...
We present the sequences at 5′ and 3′ ends of one hsp 70 gene variant which is derived from chromosomal locus 87A7. The end mRNA has also been determined. 550 bp upstream mRNA, there a very A+T rich region shown by heteroduplex analysis to be same position in other genes 9 . was found 26 after characteristic “Hogness box”. first ATG codon 250 downstream mRNA. determined termination gene. Comparisons with are discussed.
The homeotic genes in Drosophila melanogaster are aligned on the chromosome order of body segments that they affect. affecting more posterior repress anterior genes. This dominance rule must be qualified case abdominal-A (abd-A) repression by Abdominal-B (Abd-B). Animals lacking Abd-B show ectopic expression abd-A epidermis eighth abdominal segment, but not central nervous system. Repression these neuronal cells is accomplished a 92 kb noncoding RNA. "iab-8 RNA" produces micro RNA to abd-A,...
In insects, products of the male reproductive tract are essential for initiating and maintaining female post-mating response (PMR). The PMR includes changes in egg laying, receptivity to courting males, sperm storage. Drosophila, previous studies have determined that main cells accessory gland produce some required these processes. However, nothing was known about contribution gland's other secretory cell type, secondary cells. course investigating late functions homeotic gene, Abdominal-B...
Abstract Seminal proteins from the Drosophila male accessory gland induce post-mating responses (PMR) in females. The PMR comprise behavioral and physiological changes that include increased egg laying, decreased receptivity to courting males, storage use of sperm. Many these are induced by a “sex peptide” (SP) maintained SP’s binding to, slow release from, contains two secretory cell types with distinct morphological developmental characteristics. Products “main” “secondary” cells work...