A5061495027- Lung Cancer Treatments and Mutations
- Colorectal Cancer Treatments and Studies
- AI in cancer detection
- Radiomics and Machine Learning in Medical Imaging
- Head and Neck Cancer Studies
- Cancer-related Molecular Pathways
- Gastric Cancer Management and Outcomes
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Thyroid Cancer Diagnosis and Treatment
- Oral and Maxillofacial Pathology
- PARP inhibition in cancer therapy
- Epigenetics and DNA Methylation
- Ocular Oncology and Treatments
- Cancer-related gene regulation
- Cell Image Analysis Techniques
- Genomics and Chromatin Dynamics
- Microtubule and mitosis dynamics
- Cell death mechanisms and regulation
- Skin Protection and Aging
- CAR-T cell therapy research
- Prostate Cancer Treatment and Research
- Ear and Head Tumors
- Cancer, Hypoxia, and Metabolism
- Ferroptosis and cancer prognosis
University of Molise
2017-2025
University of Naples Federico II
2005-2023
Institute for Experimental Endocrinology and Oncology
2005-2021
University of Bari Aldo Moro
2019-2020
National Agricultural and Food Centre
2019-2020
Weatherford College
2019-2020
Federico II University Hospital
2015-2019
Tecnologie Avanzate (Italy)
2016
Institute of Experimental Endocrinology of the Slovak Academy of Sciences
2012
The RAS/RAF/MEK/MAPK and the PTEN/PI3K/AKT/mTOR pathways are key regulators of proliferation survival in human cancer cells. Selective inhibitors different transducer molecules these have been developed as molecular targeted anti-cancer therapies. vitro vivo anti-tumor activity pimasertib, a selective MEK 1/2 inhibitor, alone or combination with PI3K inhibitor (PI3Ki), mTOR (everolimus), multi-targeted kinase (sorafenib regorafenib), that block also BRAF CRAF, were tested panel eight lung...
Abstract Purpose: In colorectal cancer, the activation of intracellular RAS–RAF and PIK3CA–AKT pathways has been implicated in resistance to anti-EGFR mAbs. We have investigated role regorafenib, an oral multikinase inhibitor, combination with cetuximab, mAb, overcome resistance. Experimental Design: tested, vitro vivo, effects regorafenib a panel human cancer cell lines KRAS mutation (SW480, SW620, HCT116, LOVO, HCT15) or BRAF (HT29), as models intrinsic cetuximab treatment, two (GEO SW48)...
// Francesco Morra 1 , Merolla 2, 3 Virginia Napolitano 1, 2 Gennaro Ilardi Caterina Miro Simona Paladino 4 Stefania Staibano Aniello Cerrato Angela Celetti Institute for Experimental Endocrinology and Oncology, Research National Council, Naples, Italy Department of Advanced Biomedical Sciences, University "Federico II", Medicine Health Sciences "V. Tiberio", Molise, Campobasso, Molecular Medical Biotechnology, Correspondence to: Celetti, email: celetti@unina.it Keywords: CCDC6, USP7, ARFL...
Non‐small cell lung cancer (NSCLC) is the main cause of cancer‐related death worldwide and new therapeutic strategies are urgently needed. In this study, we have characterized a panel NSC lines for expression coiled‐coil‐domain containing 6 (CCDC6), tumor suppressor gene involved in apoptosis DNA damage response. We show that low CCDC6 protein levels associated with weak response to number Rad51 positive foci. Moreover, deficient cells defects repair via homologous recombination. accordance...
PReferentially expressed Antigen in Melanoma (PRAME) is a cancer testis antigen, first isolated tumor-reactive T-cell clones from metastatic melanoma patient. It has been widely studied skin pathology as an immunohistochemical marker capable of distinguishing between benign nevi and malignant melanomas. PRAME found to be also non-melanocytic tumors, including lung, breast, kidney ovarian cancer. However, less known about the diagnostic and/or prognostic role this protein uveal (UM); few...
In the fully digital Caltagirone pathology laboratory, a reverse shift from to manual workflow occurred due server outage in September 2023. Here, insights gained this unplanned transition are explored. Surveying affected pathologists and technicians revealed unanimous preferences for time-saving error-reducing capabilities of methodology. Conversely, return methods highlighted increased dissatisfaction reduced efficiency, emphasising superiority workflows. This case study underscores that...
CCDC6 gene product is a pro-apoptotic protein substrate of ATM, whose loss or inactivation enhances tumour progression. In primary tumours, the impaired function has been ascribed to rearrangements and somatic mutations in several neoplasia. Recently, low levels protein, NSCLC, have correlated with tumor prognosis. However, mechanisms responsible for variable tumors not described yet.We show that turnover regulated cell cycle dependent manner. undergoes cyclic variation phosphorylated status...
Oral squamous cell carcinoma is the most common oral cancer. In this paper, we present a performance analysis of four different deep learning-based pixel-wise methods for lesion segmentation on images. Two diverse image datasets, one training and another testing, are used to generate evaluate models segmenting images, thus allowing assess generalization capability considered network architectures. An important contribution work creation Cancer Annotated (ORCA) dataset, containing...
Background: Despite advances in uveal melanoma (UM) diagnosis and treatment, about 50% of patients develop distant metastases, thereby displaying poor overall survival. Molecular profiling has identified several genetic alterations that can stratify with UM into different risk categories. However, these are currently dispersed over multiple studies methodologies, emphasizing the need for a defined workflow will allow standardized reproducible molecular analyses. Methods: Following findings...
Background: the treatment of squamous cell carcinomas oral cavity (OSCCs) is limited by lack reliable diagnostic/prognostic, and predictive markers, as well intrinsic tumor heterogeneity. 5-azacytidine (5-AZA) offers opportunities for cancer reprogramming to develop new target-specific treatments. The protein annexin A1 (ANXA1) downregulated in head neck carcinoma (HNSCC), correlated with pathological differentiation grade. Objectives: this work aimed further investigate role ANXA1 OSCC...
In the present study, we have characterized several human thyroid cancer cell lines of different histotypes for their responsiveness to contact inhibition. We found that cells derived from differentiated carcinoma (TPC-1, WRO) arrest in G(1) phase at confluence, whereas anaplastic (ARO, FRO and FB1) continue grow after reaching confluence. Furthermore, provide experimental evidence axis, E-cadherin/beta-catenin/p27(Kip1), represents an integral part regulatory mechanism controls...
The natural history of oral squamous cell carcinomas (OSCCs) is variable and difficult to predict. This study aimed assess the value expression poly(ADP-ribose) polymerase 1 (PARP-1), chromatin assembly factor-1 (CAF-1)/p60 stem markers CD133, CD166, CD44, CD44v6 nestin as outcome progression-free survival in OSCC patients.Clinical data were collected from 66 patients (41 male 25 female, aged 29-92 years) who underwent surgery for tongue, floor, lips, palate. During follow-up (range: 12-131...
CCDC6 was originally identified in chimeric genes caused by chromosomal translocation involving the RET proto-oncogene some thryoid tumors mostly upon ionizing radiation exposure. Recognised as a pro-apoptotic phosphoprotein that negatively regulates CREB1-dependent transcription, is an ATM substrate responsive to genotoxic stress. Here we report following stress, loss or inactivation of cancers carry fusion, accelerates dephosphorylation pH2AX S139, resulting defective G2 arrest and...
Up-to-date, several molecular markers of prognosis have been studied in Oral Squamous Cell Carcinoma (OSCC), but none entered the clinical setting. Therapy OSCC tumors mainly relies on surgery, radiotherapy and partially chemotherapy; there is an urgent need for biomarkers able to better stratify patients' risk address targeted therapeutic strategies. The role immune response pathogenesis biological behavior has investigated by authors, promising results obtained with checkpoint inhibitors....
The muscle invasive form of urothelial bladder cancer (UBC) is a deadly disease. Currently, the therapeutic approach UBC mostly based on surgery and standard chemotherapy. Biomarkers to establish appropriate drugs usage are missing. Deficiency tumor suppressor CCDC6 determines PARP-inhibitor sensitivity. levels modulated by deubiquitinase USP7. In this work we scored USP7 expression in primary evaluated correlation with effects PARP-inhibitors combined inhibitor, P5091, vitro. Since could be...
Uveal melanoma (UM) is the most common intraocular tumor in adults. Despite sharing name and similar morphological features with cutaneous (CM), it an entirely different neoplasia a particular genetic background clinical behavior. CDKN2A gene located at chromosome 9p21, encoding for P16INK4a P14(ARF) proteins, whose role as suppressor has been clearly defined many malignant tumors. frequently presents germline mutations familial CM epigenetic downregulation considerable percentage of...
We introduce a machine learning-based analysis to predict the immunohistochemical (IHC) labeling index for cell proliferation marker Ki67/MIB1 on cancer tissues based morphometrical features extracted from hematoxylin and eosin (H&E)-stained formalin-fixed, paraffin-embedded (FFPE) tumor tissue samples. provided proof-of-concept prediction of IHC positivity cells through definition quantitation single nuclear features. In first instance, we set our digital framework Ki67/MIB1-stained OSCC...
Pathological Anatomy is moving toward computerizing processes mainly due to the extensive digitization of histology slides that resulted in availability many Whole Slide Images (WSIs). Their use essential, especially cancer diagnosis and research, raises pressing need for increasingly influential information archiving retrieval systems. Picture Archiving Communication Systems (PACSs) represent an actual possibility archive organize this growing amount data. The design implementation a robust...