Abstract The integration of metasurfaces on the tip optical fibers enables advanced wavefront manipulations in Lab‐on‐Fiber application scenarios, and brings about new degrees freedom that can be exploited for optimizing surface sensitivity to local variations refractive index. Here, a novel biosensing platform is reported based phase‐gradient plasmonic metasurface an fiber, able detect biomolecular interactions with very high sensitivity. Specifically, capability proposed low concentrations...

We developed an immunoassay platform for the detection of human Thyroglobulin (Tg) to be integrated with fine-needle aspiration biopsy early lymph node metastases in thyroid cancer patients. The sensing detects Tg by a sandwich involving self-assembled surface-enhanced Raman scattering (SERS) substrate assisted functionalized gold nanoparticles that provide additional signal amplification and improved molecular specificity. Specifically, SERS-active substrates were Capture antibodies...

Insulin‐like growth factor‐1 (IGF‐1) mediates some of hormone anabolic functions through its receptor, IGF‐1R. Following ligand binding, intracellular signaling pathways are activated favouring proliferation, cell survival, tissue growth, development, and differentiation. IGF‐1 is included in the World Anti‐Doping Agency Prohibited List. While evidence for as performance‐enhancing substrate healthy humans still weak, clinical studies demonstrated that endogenous hormone/IGF‐1 excess...

Abstract The creation of SERS‐active substrates is investigated by self‐assembling hierarchical structures plasmonic‐assisted nanospheres (HSNs). architecture exploits the advantages well‐known ordered configuration a hexagonally closed‐packed array (CPA) nanospheres. A further layer upper used to provide regular and intense surface hotspots, located at nanogaps between neighboring Numerical analysis carried out predict SERS performances identify more promising configurations, offering...

Label-free plasmonic biosensors have demonstrated promising capabilities as diagnostic tools for the detection of different target analytes. The overall analytical sensitivity and specificity these systems depend on sensor surface biofunctionalization, since it influences their interactions with molecules determines applicability to biomolecular in complex matrices. In this study, we report development Au biosensor by integrating immunosandwich sensing approach into a Surface Plasmon...

We investigated the possibility of using Raman spectroscopy assisted by artificial intelligence methods to identify liver cancer cells and distinguish them from their Non-Tumor counterpart. To this aim, primary (40 Tumor 40 cells) obtained resected hepatocellular carcinoma (HCC) tumor tissue adjacent non-tumor area (negative control) were analyzed micro-spectroscopy. Preliminarily, morphologically spectrally. Then, three machine learning approaches, including multivariate models neural...

Abstract Anabolic androgenic steroids, a class of steroid hormones related to testosterone, are natural ligands androgen receptor (AR), member the nuclear superfamily ligand‐activated transcription factors. AR binds specific DNA elements, known as androgen–response elements. Testosterone, main male sexual hormone, directly and indirectly, through conversion into dihydrotestosterone (DHT), its more active metabolite. steroids frequently detected in urine doped athletes; their consumption is...

The abuse of mixed or combined performance-enhancing drugs is widespread among athletes and amateurs, adults adolescents. Clinical studies demonstrated that misuse these doping agents associated with serious adverse effects to many organs in human. Previously, we human peripheral blood lymphocytes high doses anabolic androgenic steroids, such as dihydrotestosterone (DHT) growth factors, insulin-like factor-1 (IGF-1), have at gene protein levels. Supraphysiological treatments DHT IGF-1...

Lab-on-fiber (LoF) optrodes offer several advantages over conventional techniques for point-of-care platforms aimed at real-time and label-free detection of clinically relevant biomarkers. Moreover, the easy integration LoF in medical needles, catheters, nano endoscopes unique potentials vivo biopsies tumor microenvironment assessment. The main barrier to translating vision close reality is need further lower final limit developed optrodes. For immune-biosensing purposes, assay sensitivity...

We investigated the creation of SERS-active hierarchical substrates based on self-assembled nanospheres (HSNs). demonstrate how "hierarchical" approach could be systematically exploited to extend SERS hotspots into third dimension, by enhancing hot-spots spatial density and intensity. The proposed take advantage single layer hexagonal closed packed array (CPA). An additional upper obtain dense intense hot spots pattern is employed. To predict performance identify promising architectures, a...

Lab-on-Fiber (LoF) technology is a research field aimed at transforming simple optical fiber into multifunctional probe, which exploits enhanced light-matter interaction for variety of applications, with special aptitude biosensing. An attractive thread in this scenario the integration plasmonic metasurfaces onto an tip, known as "meta-tips", leading to development new generation highly sensitive optrodes. Here we report on latest achievements concerning investigation LoF probes assisted by...

Abstract Background Breast cancer (BC) is a heterogeneous neoplasm characterized by several subtypes. One of the most aggressive with high metastasis rates presents overexpression human epidermal growth factor receptor 2 (HER2). A quantitative evaluation HER2 levels essential for correct diagnosis, selection appropriate therapeutic strategy and monitoring response to therapy. Results In this paper, we propose synergistic use SERS Raman technologies identification expressing cells its...

<title>Abstract</title> <bold>Background</bold> Breast cancer (BC) is a heterogeneous neoplasm characterized by several subtypes. One of the most aggressive with high metastasis rates presents overexpression human epidermal growth factor receptor 2 (HER2). A quantitative evaluation HER2 levels essential for correct diagnosis, selection appropriate therapeutic strategy and monitoring response to therapy. <bold>Results</bold> In this paper, we propose synergistic use SERS Raman technologies...

The ability to make an accurate diagnosis at the time of treatment is crucial for many diseases. However, current standard diagnostic procedures can only be performed in specialised healthcare facilities. To bring methods from a laboratory point treatment, alternative have been proposed. One them surfaceenhanced Raman scattering (SERS), which offers advantageous features such as high sensitivity biotarget detection and higher accuracy. Here, we developed advanced SERS platform...

Hepatocellular carcinoma (HCC), the most common form of primary liver cancer, represents a global health challenge due to its complexity and limitations current diagnostic techniques. By combining Raman spectroscopy Artificial Intelligence (AI), we have succeeded in classifying tumor cells. In fact, performed first spectral analysis based on characterization differentiation between uncultured human cells derived from resected HCC tissue adjacent non-tumor counterpart. Biochemical collected...

Here we have developed an advanced surface-enhanced Raman scattering (SERS) platform that enables the ultrasensitive, rapid and highly specific identification of tumour biomarkers in liquid biopsies. Our focus is on detection Thyroglobulin (Tg), most important biomarker for diagnosis prognosis thyroid cancer. Specifically, SERS-active substrates fabricated by nanosphere lithography chip or tips optical fiber (OF) were functionalised with Tg Capture antibodies. Gold nanoparticles...

Assessing HER2 expression in breast cancer cells holds significant diagnostic and prognostic importance. Traditional methods like immunohistochemistry situ hybridization suffer from low sensitivity misclassification rates. In this frame, techniques such as vibrational microscopies can ensure, together with costs analytical speed, both high accuracy precision. Herein, we propose a combined Raman SERS approach for characterizing 4 cell lines normal varying levels. We show that spectroscopy...

This article was originally published in Proteomics 2010, 10, 3165–3175, DOI 10.1002/pmic.201000079

In this work, we propose a novel immunoassay platform for the detection of human Thyroglobulin (Tg) to be integrated with fine-needle aspiration biopsy early identification lymph node metastases in thyroid cancer patients. The sensing detects Tg (a well-known biomarker classification metastatic nodes related cancer) by sandwich involving self-assembled surface-enhanced Raman scattering (SERS) substrate assisted and empowered functionalized gold nanoparticles enabling additional signal...