A5031360676- Immunotherapy and Immune Responses
- Multiple Sclerosis Research Studies
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Reproductive System and Pregnancy
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Cytokine Signaling Pathways and Interactions
- Liver Disease Diagnosis and Treatment
- Polyomavirus and related diseases
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- Psoriasis: Treatment and Pathogenesis
- Inflammatory Bowel Disease
- Immune responses and vaccinations
- Cancer Research and Treatments
- Glycosylation and Glycoproteins Research
- Systemic Lupus Erythematosus Research
- Diabetes and associated disorders
- Animal Virus Infections Studies
- HER2/EGFR in Cancer Research
- Adenosine and Purinergic Signaling
- Acute Lymphoblastic Leukemia research
University of Turin
2015-2024
Ospedale San Luigi Gonzaga
2006-2019
Gonzaga University
2016
Azienda Ospedaliera Citta' della Salute e della Scienza di Torino
2013-2016
University of Osijek
2016
Psychiatric Hospital Sveti Ivan
2016
Charles University
2016
University Hospital Centre Zagreb
2016
University of Cagliari
2016
Università di Camerino
2014
The mechanisms responsible for the evolution of steatosis towards NASH (non-alcoholic steatohepatitis) and fibrosis are not completely defined. In present study we evaluated role CD4(+) T-helper (Th) cells in this process. We analysed infiltration different subsets Th C57BL/6 mice fed on a MCD (methionine choline-deficient) diet, which is model reproducing all phases human progression. There was an increase Th17 at beginning development NASH-fibrosis transition, whereas levels Th22 peaked...
// Moitza Principe 1,2,* , Patrizia Ceruti Neng-Yao Shih 3,* Michelle S. Chattaragada 1,2 Simona Rolla Laura Conti 2,4 Marco Bestagno 5 Lorena Zentilin Sheng-Hui Yang 6 Paola Migliorini 7 Cappello Oscar Burrone and Francesco Novelli 1 Center for Experimental Research Medical Studies (CeRMS), Azienda Universitaria Ospedaliera Città della Salute e Scienza di Torino, Turin, Italy 2 Department of Molecular Biotechnology Health Sciences, University 3 National Institute Cancer Research,...
Abstract Th1 and Th17 cells have been considered as effectors in mouse EAE the human counterpart, MS. Recently, IL-22, a Th17-related, proinflammatory cytokine, has associated with new Th cell subset, defined Th22, involved chronic inflammatory conditions, such psoriasis; role of IL-22 MS not yet elucidated. Here, we report that similar to cells, number Th22 increased PB CSF RR patients, especially during active phases disease. However, opposed expansion occurred before were found be...
<h3>Objective:</h3> To analyze changes in T-helper (Th) subsets, T-regulatory (Treg) cell percentages and function, mRNA levels of immunologically relevant molecules during a 24-month follow-up after alemtuzumab treatment patients with relapsing-remitting multiple sclerosis (RRMS). <h3>Methods:</h3> Multicenter 29 alemtuzumab-treated RRMS the Comparison Alemtuzumab Rebif Efficacy Multiple Sclerosis (CARE-MS) I CARE-MS II trials. Peripheral blood (PB) samples were obtained at months 0, 6, 12,...
Abstract Central tolerance to tumor-associated Ags is an immune-escape mechanism that significantly limits the TCR repertoires available for tumor eradication. The expanded in wild-type BALB/c and rat-HER-2/neu (rHER-2) transgenic BALB-neuT mice following DNA immunization against rHER-2 were compared by spectratyping variable (V)β joining (J)β CDR 3. Following immunization, raised a strong response. Every mouse used one or more CD8+ T cell rearrangements of Vβ9-Jβ1.2 segments characterized...
While much experimental data shows that vaccination efficiently inhibits a subsequent challenge by transplantable tumor, its ability to inhibit the progress of autochthonous preneoplastic lesions is virtually unknown. In this article, we show combined DNA and cell vaccine persistently such in murine HER-2/neu mammary carcinogenesis model. At 10 weeks age, all ten gland samples from HER-2/neu–transgenic mice displayed foci hyperplasia progressed invasive tumors. Vaccination with plasmids...
Tobacco smoking is known to impact circulating levels of major immune cells populations, but its effect on specific cell subsets remains poorly understood. Here, using high-resolution data from 223 healthy women (25 current and 198 never smokers), we investigated the association between status 35,651 traits capturing subset frequencies. Our results confirmed that active tobacco associated with increased frequencies CD8+ T expressing CD25 activation marker. Moreover, identified novel...
PDAC (pancreatic ductal adenocarcinoma) is the fifth leading cause of cancer-related death. The causes this cancer remain unknown, but increasing evidence indicates a key role host immune response and cytokines in human carcinogenesis. Intra-tumoral IL (interleukin)-22 levels have been shown to be elevated patients. However, little known regarding expression clinical relevance Th22 cells and, furthermore, which TILs (tumour-infiltrating lymphocytes) are main producers IL-22 unknown. In...
Increasing evidence supports the anti-inflammatory role of estrogens in Multiple Sclerosis (MS), originating from observation reduction relapse rates among women with MS during pregnancy, but molecular mechanisms are still not completely understood. Using an integrative data analysis, we identified T helper (Th) 17 and regulatory (Treg) cell-type-specific regions (CSR) regulated by estrogen receptor alpha (ERα). These CSRs were validated polarized Th17 healthy donors (HD) peripheral blood...
Hemopexin (Hx) is an acute-phase protein synthesized by hepatocytes in response to the proinflammatory cytokines IL-6, IL-1β, and TNF-α. Hx plasma with highest binding affinity heme controls heme-iron availability tissues also T lymphocytes, where it modulates their responsiveness IFN-γ. Recent data have questioned regarding anti-inflammatory role of Hx, a that may be both heme-binding dependent independent. The aim this study was investigate development cell-mediated inflammatory autoimmune...
Abstract Purpose: Despite the great success of HER2 vaccine strategies in animal models, effective clinical results have not yet been obtained. We studied feasibility using DNA coding for chimeric rat/human as a tool to break unresponsiveness T cells from patients with HER2-overexpressing tumors (HER2-CP). Experimental Design: Dendritic (DCs) generated breast (n = 28) and pancreatic 16) cancer were transfected plasmids that express human or heterologous rat sequences separate constructs...
The Mediterranean Diet (MD) is described in the literature as a beneficial dietary pattern for neurodegenerative diseases such Multiple Sclerosis (MS). objective of this study was to evaluate habits people with MS (pwMS) and test whether adherence MD could have an impact on severity disease measured score (MSSS). Adherence assessed 31 PwMS using Screener (MEDAS), Pyramid-based Score (PyrMDS) index, Italian Index (IMI), their eating were recorded food diary one-year follow-up. When data...
Rat (r)Erbb2 transgenic BALB-neuT mice genetically predestined to develop multiple invasive carcinomas allow an assessment of the potential a vaccine against stages cancer progression. Because rErbb2 expression in thymus and its overexpression mammary gland, CD8(+) T cell clones reacting at high avidity with dominant epitopes are deleted these mice. In diffuse situ lesions almost palpable carcinomas, temporary regulatory cells depletion combined anti-rErbb2 markedly enhanced Ab response...
IFN-β inhibits the expansion of Th17 cells in active multiple sclerosis (AMS), and this might contribute to improve clinical symptoms. The effectiveness inhibition, however, requires intact IFN-γ signaling T cells. In study, we report that both mRNA cell surface expression chain receptor (IFN-γR2) its cognate tyrosine kinase JAK2 are enhanced peripheral blood clones from patients with AMS compared those inactive (IMS) or healthy subjects (HS). decreased frequency proliferation patients....
Abstract Background Multiple Sclerosis (MS) represents nowadays in Europe the leading cause of non-traumatic disabilities young adults, with more than 700,000 EU cases. Although huge strides have been made over years, MS etiology remains partially unknown. Furthermore, presence various endogenous and exogenous factors can greatly influence immune response different individuals, making it difficult to study understand disease. This becomes evident a personalized-fashion when medical doctors...
Alemtuzumab is highly effective in the treatment of patients with relapsing multiple sclerosis (PwRMS) and selectively targets CD52 antigen, a consequent profound lymphopenia, particularly CD4+ T lymphocytes. However, immunological basis its long-term efficacy has not been clearly elucidated.We followed up 29 alemtuzumab-treated RMS over period 72 months studied reconstitution their cell subsets by means phenotypic functional analysis through mRNA-related molecule expression, comparing them...