A5000419040- Ion Channels and Receptors
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Respiratory and Cough-Related Research
- Postharvest Quality and Shelf Life Management
- Olfactory and Sensory Function Studies
- Asthma and respiratory diseases
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Insect Pest Control Strategies
- Phytochemicals and Antioxidant Activities
- Neuroscience of respiration and sleep
- Herbal Medicine Research Studies
- Free Radicals and Antioxidants
- Poisoning and overdose treatments
- Pediatric health and respiratory diseases
- Invertebrate Immune Response Mechanisms
- Exercise and Physiological Responses
- Lipid Membrane Structure and Behavior
- Circadian rhythm and melatonin
- Plant Toxicity and Pharmacological Properties
- Influenza Virus Research Studies
- Respiratory viral infections research
- Biochemical effects in animals
- Dietary Effects on Health
VIB-KU Leuven Center for Brain & Disease Research
2018-2024
Saarland University
2020-2023
KU Leuven
2013-2023
Lipopolysaccharides (LPS), the major components of wall gram-negative bacteria, trigger powerful defensive responses in airways via mechanisms thought to rely solely on Toll-like receptor 4 (TLR4) immune pathway. Here we show that airway epithelial cells display an increase intracellular Ca2+ concentration within seconds LPS application. This response occurs a TLR4-independent manner, activation transient potential vanilloid cation channel (TRPV4). We found TRPV4 mediates immediate...
Detecting pathogens and mounting immune responses upon infection is crucial for animal health. However, these come at a high metabolic price (McKean Lazzaro, 2011, Kominsky et al., 2010), avoiding before may be advantageous. The bacterial endotoxins lipopolysaccharides (LPS) are important system cues (Abbas 2014), but it remains unknown whether animals possess sensory mechanisms to detect them prior infection. Here we show that Drosophila melanogaster display strong aversive LPS gustatory...
Mucociliary clearance through coordinated ciliary beating is a major innate defense removing pathogens from the lower airways, but pathogen sensing and downstream signaling mechanisms remain unclear. We identified virulence-associated formylated bacterial peptides that potently stimulated ciliary-driven transport in mouse trachea. This response was independent of formyl peptide taste receptors depended on key transduction genes. Tracheal cholinergic chemosensory cells expressed these genes,...
Host-derived succinate accumulates in the airways during bacterial infection. Here, we show that luminal activates murine tracheal brush (tuft) cells through a signaling cascade involving receptor 1 (SUCNR1), phospholipase Cβ2, and cation channel transient potential subfamily M member 5 (TRPM5). Stimulated then trigger long-range Ca 2+ wave spreading radially over epithelium sequential process. First, release acetylcholine, which excites nearby via muscarinic acetylcholine receptors. From...
Allyl isothiocyanate (AITC; aka, mustard oil) is a powerful irritant produced by <i>Brassica</i> plants as defensive trait against herbivores and confers pungency to wasabi. AITC widely used experimentally an inducer of acute pain neurogenic inflammation, which are largely mediated the activation nociceptive cation channels transient receptor potential ankyrin 1 vanilloid (TRPV1). Although it generally accepted that electrophilic agents activate these through covalent modification cytosolic...
The cation channel TRPA1 transduces a myriad of noxious chemical stimuli into nociceptor electrical excitation and neuropeptide release, leading to pain neurogenic inflammation. Despite emergent evidence that is regulated by the membrane environment, it remains unknown whether this localizes in microdomains or interacts with cholesterol. Using total internal reflection fluorescence microscopy density gradient centrifugation we found mouse preferably cholesterol-rich domains functional...
Asthma may be induced by chemical sensitisers, via mechanisms that are still poorly understood. This type of asthma is characterised airway hyperreactivity (AHR) and little inflammation. Since potent such as toluene-2,4-diisocyanate (TDI), also sensory irritants, it suggested chemical-induced relies on neuro-immune mechanisms.We investigated the involvement transient receptor potential channels (TRP) A1 V1, major chemosensors in airways, mast cells, known for their ability to communicate...
25-Hydroxyvitamin D (25OHD) is a partial agonist of TRPV1 whereby 25OHD can weakly activate yet antagonize the stimulatory effects full agonists capsaicin and oleoyl dopamine. binds to within same vanilloid binding pocket as capsaicin. inhibits potentiating PKC-mediated activity. reduces T-cell activation trigeminal neuron calcium signalling mediated by These results provide evidence that novel receptor for biological actions vitamin in addition well-documented upon nuclear receptor. The may...
Background: The transient receptor potential ankyrin 1 (TRPA1) cation channels function as broadly-tuned sensors of noxious chemicals in many species. Recent studies identified four functional TRPA1 isoforms Drosophila melanogaster (dTRPA1(A) to (D)), but their responses non-electrophilic are yet be fully characterized. Methods: We determined the behavioral adult flies mammalian activators citronellal and menthol, characterized effects these compounds on all dTRPA1 channel using...
The Transient Receptor Potential Ankyrin 1 (TRPA1) cation channels function as broadly-tuned sensors of noxious chemicals in many species. Recent studies identified four functional TRPA1 isoforms Drosophila melanogaster (dTRPA1(A) to (D)), but their responses non-electrophilic are yet be fully characterized. Methods: We determined the behavioral adult flies mammalian activators citronellal and menthol, characterized effects these compounds on all dTRPA1 channel using intracellular Ca2+...