A5000243872- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Air Quality and Health Impacts
- Cancer-related molecular mechanisms research
- Allergic Rhinitis and Sensitization
- MicroRNA in disease regulation
- Eosinophilic Esophagitis
- Pediatric health and respiratory diseases
- Neonatal Respiratory Health Research
- Circular RNAs in diseases
- Immune Cell Function and Interaction
- Energy and Environment Impacts
- Inhalation and Respiratory Drug Delivery
- Immune Response and Inflammation
- Helicobacter pylori-related gastroenterology studies
- Biomarkers in Disease Mechanisms
- Immunotherapy and Immune Responses
- Respiratory and Cough-Related Research
- Noise Effects and Management
- Respiratory Support and Mechanisms
- Occupational exposure and asthma
- RNA modifications and cancer
- Indoor Air Quality and Microbial Exposure
- Plant-Microbe Interactions and Immunity
Ghent University Hospital
2016-2025
Ghent University
2001-2023
Translational Research in Oncology
2009-2022
Vlaams Instituut voor Biotechnologie
2002
Chronic obstructive pulmonary disease (COPD) is mainly caused by cigarette smoking, and characterized an increase in inflammatory cells the airways tissue. The chemokine receptor CCR6 its ligand MIP-3alpha/CCL20 may be involved recruitment of these cells. To investigate role pathogenesis COPD, we analyzed responses knockout (KO) wild-type mice upon smoke (CS) exposure. Both subacute chronic exposure to CS induced innate adaptive immune system bronchoalveolar lavage, both KO mice. However,...
T-regulatory cells (T(reg)) are important in balancing immune responses and maintaining peripheral tolerance. Current evidence suggests that asthma is characterized by a relative deficiency T(reg), allowing T helper 2 to expand. In this study, we aimed evaluate circulating defined the protein FOXP3, both control subjects patients with stable asthma.Peripheral blood mononuclear (PBMC) of (n = 14) asthmatic 29) were labeled for CD4, CD25, intracellular FOXP3 analyzed using flow cytometry....
Background Innate lymphoid cells (ILC) are a new family of innate immune that have emerged as important regulators tissue homeostasis and inflammation. However, limited data available concerning the relative abundance characteristics ILC in human lung. Methods The aim this study was to characterize enumerate different subsets lung by multi-color flow cytometry. Results Within CD45+ Lin- CD127+ pulmonary population, we identified group 1 (ILC1), 2 (ILC2) 3 (ILC3) using specific surface...
Aberrant expression of microRNAs (miRNAs) can have a detrimental role in disease pathogenesis.To identify dysregulated miRNAs lung tissue patients with chronic obstructive pulmonary (COPD).We performed miRNA and mRNA profiling using high throughput stem-loop reverse-transcriptase quantitative polymerase chain reaction microarray, respectively, on 30 (screening cohort) encompassing 8 never-smokers, 10 smokers without airflow limitation, 12 COPD. Differential miRNA-218-5p (miR-218-5p) was...
Experimental models are critical for the understanding of lung health and disease indispensable drug development. However, pathogenetic clinical relevance is often unclear. Further, use animals in biomedical research controversial from an ethical perspective. The objective this task force was to issue a statement with recommendations about by facilitating in-depth discussions between respiratory scientists, provide overview literature on available models. Focus put their specific benefits...
Extracellular RNAs present in biofluids have emerged as potential biomarkers for disease. Where most studies focus on blood-derived fluids, other may be more informative. We an atlas of messenger, circular, and small RNA transcriptomes a comprehensive collection 20 human biofluids. By means synthetic spike-in controls, we compare content across biofluids, revealing 10,000-fold difference concentration. The circular fraction is increased compared to tissues. Each biofluid transcriptome...
Dendritic cells (DCs) appear to be strategically implicated in allergic diseases, including asthma. Matrix metalloproteinase (MMP)-9 mediates transmigration of inflammatory leukocytes across basement membranes. This study investigated the role MMP-9 airway DC trafficking during allergen-induced inflammation. gene deletion affected pulmonary DCs a specific way: only into lumen was impaired, whereas DC-mediated transport Ag thoracic lymph nodes remained unaffected. In parallel, local...
Dendritic cells (DC) have a central role in the initiation of adequate immune responses. They recognize pathogens by means Toll-like receptors (TLR) and link innate to adaptive responses releasing proinflammatory cytokines inducing T cell proliferation. We conducted this study evaluate expression function TLR on human lung DC subsets their stimulatory capacity. gene pulmonary was evaluated RT-PCR, while protein analyzed flow cytometry. investigated cytokine release response different...
Abstract Increased numbers of pulmonary dendritic cells (DCs) are recruited to the lungs during allergic airway inflammation and contribute maintenance inflammatory immune response. The chemokine receptors that directly control DC accumulation into largely unknown. To explore this issue, we generated mixed bone marrow chimeric mice containing both wild-type knockout for a given receptor. After induction inflammation, specifically tracked compared receptor vs populations through various lung...
Murine asthma models suggest that failure of immune tolerance rather than a defective T helper cell type 1 (Th1) immunity underlies the biology Th2-driven allergen-induced airway disease. Intriguingly, prolonged exposures can result in full waning inflammation. The mechanisms underlying this observation are not understood. We hypothesized fading inflammation is regulatory processes, characterized by altered dendritic (DC)-T interactions. First, we implemented model which mice developed When...
Abstract Inhalation of diesel exhaust particles (DEP) induces an inflammatory reaction in the lung; however, mechanisms are largely unclear. IL-1β/IL-1RI signaling is crucial several lung responses. Typically, caspase-1 activated within NLRP3 inflammasome that recognizes damage-associated molecular patterns, which results cleavage pro–IL-1β into mature IL-1β. In this study, we hypothesized NLRP3/caspase-1/IL-1β pathway critical DEP-induced inflammation. Upon DEP exposure, IL-1RI knockout...
BackgroundAlthough the prominent role of TH2 cells in type 2 immune responses is well established, newly identified innate lymphoid (ILC2s) can also contribute to orchestration allergic responses. Several experimental and epidemiologic studies have provided evidence that allergen-induced airway be further enhanced on exposure environmental pollutants, such as diesel exhaust particles (DEPs). However, components pathways responsible remain incompletely known.ObjectiveWe sought investigate...
Background Receptor-interacting protein kinase 1 (RIPK1) is a key mediator of regulated cell death (including apoptosis and necroptosis) inflammation, both drivers COPD pathogenesis. We aimed to define the contribution RIPK1 kinase-dependent inflammation in pathogenesis COPD. Methods assessed expression single-cell RNA sequencing (RNA-seq) data from human mouse lungs, validated levels lung tissue patients via immunohistochemistry. Next, we consequences genetic pharmacological inhibition...
Patients with chronic lung disease suffer from persistent inflammation and are typically colonized by pro-inflammatory pathogenic bacteria. Besides these pathogens, a wide variety of commensal species is present in the lower airways but their role unclear. Here, we show that microbiota contains several able to inhibit activation NF-κB pathway production interleukin 8 (IL-8), triggered lipopolysaccharide (LPS) or H 2 O , physiologically relevant three-dimensional (3D) epithelial cell model....