A5026294960- IL-33, ST2, and ILC Pathways
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- MicroRNA in disease regulation
- HIV/AIDS Research and Interventions
- Immune Cell Function and Interaction
- Eosinophilic Esophagitis
- Phytochemistry and Biological Activities
- Circular RNAs in diseases
- Asthma and respiratory diseases
- Cancer-related molecular mechanisms research
- Air Quality and Health Impacts
- Essential Oils and Antimicrobial Activity
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Nanoparticle-Based Drug Delivery
- Pediatric health and respiratory diseases
- Inhalation and Respiratory Drug Delivery
- Plant chemical constituents analysis
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Occupational exposure and asthma
- HIV-related health complications and treatments
Ghent University Hospital
2017-2025
Ghent University
2024
Persistent latent reservoirs of intact HIV-1 proviruses, capable rebounding despite suppressive antiretroviral therapy (ART), hinder efforts towards an cure. Hence, assays specifically quantifying proviruses are crucial to assess the impact curative interventions. Two recent have been utilized in clinical trials: proviral DNA assay (IPDA) and quadruplex quantitative PCR (Q4PCR). While IPDA is more sensitive due amplifying short fragments, it may overestimate fractions by relying only on...
Eryngium campestre and E. amethystinum are thorny herbs belonging to the Apiaceae family spontaneously growing in stony pastures dry meadows, preferentially on calcareous substrates. In Mediterranean countries, these plants have been used as a food or traditional remedies treat various ailments. present work, we analyzed chemical composition of essential oils distilled from aerial parts by GC-FID GC/MS, evaluated their cytotoxic effects panel human cancer cells, namely A375 (human malignant...
Clinical and experimental studies have identified a crucial role for IL-33 its receptor ST2 in allergic asthma. Inhalation of traffic-related pollutants, such as diesel exhaust particles (DEP), facilitates the development asthma can cause exacerbations However, it is unknown whether IL-33/ST2 signalling contributes to enhancing effects air pollutants on airway responses.We aim investigate functional DEP-enhanced responses, using an established murine model.C57BL/6J mice were exposed saline,...
Abstract Objectives Innate lymphoid cells (ILCs) secrete cytokines, such as IFN‐γ, IL‐13 and IL‐17, which are linked to chronic obstructive pulmonary disease (COPD). Here, we investigated the role of ILCs in COPD pathogenesis. Methods Lung ILC subsets control subjects were quantified using flow cytometry associated with clinical parameters. Tissue localisation T‐cell was determined by immunohistochemistry. Mice exposed air or cigarette smoke (CS) for 1, 4 24 weeks investigate whether numbers...
Rationale: Tissue-resident natural killer (trNK) cells have been identified in numerous organs, but little is known about their functional contribution to respiratory immunity, particular during chronic lung diseases such as obstructive pulmonary disease (COPD). Objectives: To investigate the phenotype and antiviral responses of trNK murine cigarette smoke–induced experimental COPD human parenchyma from donors. Methods: Mice were exposed smoke for 12 weeks induce COPD-like disease. Lung cell...
Abstract Background Reducing the number of active compounds for lifelong human immunodeficiency virus (HIV) treatment is interest, especially to reduce potential long-term side effects. So far, available data assessing viral control support robustness and safety 2DR (2-drug regimen) antiretroviral therapy compared 3DR. However, further in-depth investigations reservoirs are mandatory guarantee these regimens regarding stable intact HIV-1 DNA copies, RNA transcripts, sustained immunological...
Background Occupational asthma, induced by workplace exposures to low molecular weight agents such as toluene 2,4-diisocyanate (TDI), causes a significant burden patients and society. Little is known about innate lymphoid cells (ILCs) in TDI-induced asthma. A critical regulator of ILC function microRNA-155, microRNA associated with Objective To determine whether TDI exposure modifies the number ILCs lung microRNA-155 contributes airway inflammation hyperresponsiveness. Methods C57BL/6...
Abstract Persistent latent reservoirs of intact HIV-1 proviruses, capable rebounding despite suppressive ART, hinder efforts towards an cure. Hence, assays specifically quantifying proviruses are crucial to assess the impact curative interventions. Clinical trials have utilized two recent assays: proviral DNA assay (IPDA) and Q4PCR. While IPDA is more sensitive due amplifying short fragments, it may overestimate fractions by relying only on small regions. Q4PCR sequencing-based its...
Studies using miR-155 deficient animals have established a role for in inflammatory responses. MicroRNA profiling revealed increased expression lungs of smokers with and without COPD cigarette smoke (CS)-exposed mice. The aim this study was to examine the model CS-induced inflammation. B6.Cg-Mir155<sup>tm1.1Rsky/J</sup> (miR-155 KO) mice C57BL/6J (Wild Type, WT) were exposed CS or air during 4 weeks. After period, sacrificed profile broncho-alveolar lavage (BAL) fluid analysed. exposure WT...
<b>Background:</b> Whereas occupational asthma, induced by workplace exposures to allergens or respiratory sensitizers, such as toluene 2,4-diisocyanate (TDI), causes a significant burden patients and society, its pathogenesis still needs be fully elucidated. Although the adaptive responses have been characterized previously, there is little information about role of innate lymphoid cells (ILC) in TDI-induced asthma. A critical regulator ILC function microRNA-155 (miR-155), miR associated...
<b>Background:</b> Whereas occupational asthma, induced by workplace exposures to allergens or respiratory sensitizers, such as toluene 2,4-diisocyanate (TDI), causes a significant burden patients and society, its pathogenesis still needs be fully elucidated. Although the adaptive responses have been characterized previously, there is little information about role of innate lymphoid cells (ILC) in TDI-induced asthma. A critical regulator ILC function microRNA-155 (miR-155), miR associated...