A5090217307- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Virus-based gene therapy research
- Virology and Viral Diseases
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Viral Infections and Immunology Research
- Cellular transport and secretion
- Viral gastroenteritis research and epidemiology
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Infectious Encephalopathies and Encephalitis
- Retinal and Optic Conditions
- HIV Research and Treatment
- Retinal Development and Disorders
- Ubiquitin and proteasome pathways
- COVID-19 epidemiological studies
- interferon and immune responses
- Vector-borne infectious diseases
- Zoonotic diseases and public health
- Long-Term Effects of COVID-19
- Herpesvirus Infections and Treatments
- Neurological diseases and metabolism
- RNA regulation and disease
- Hereditary Neurological Disorders
Inserm
2016-2025
Pathogenesis and Control of Chronic and Emerging Infections
2021-2025
Université de Montpellier
2015-2025
Établissement Français du Sang
2016-2025
Malden Public Schools
2023
Centre Hospitalier Universitaire de Montpellier
2021
Hôpital Arnaud de Villeneuve
2017-2020
Lomonosov Moscow State University
2018-2019
Centre National de la Recherche Scientifique
2005-2018
Institut de Génétique Moléculaire de Montpellier
2004-2018
The recent Zika virus (ZIKV) epidemic has highlighted the poor knowledge on its physiopathology. Recent studies showed that ZIKV of Asian lineage, responsible for this international outbreak, causes neuropathology in vitro and vivo. However, two African lineages exist is currently found circulating Africa. original strain was also suggested to be neurovirulent but laboratory usage been criticized due multiple passages. In study, we compared French Polynesian (Asian) an isolated Central...
Abstract Infection with Usutu virus (USUV) has been recently associated neurologic disorders, such as encephalitis or meningoencephalitis, in humans. These findings indicate that USUV is a potential health threat. We report an acute human infection France putatively clinical diagnosis of idiopathic facial paralysis.
Noradrenergic neurons of the brainstem extend projections throughout neuraxis to modulate a wide range processes including attention, arousal, autonomic control and sensory processing. A spinal projection from locus coeruleus (LC) is thought regulate nociceptive To characterize selectively manipulate pontospinal noradrenergic in rats, we implemented retrograde targeting strategy using canine adenoviral vector express channelrhodopsin2 (CAV2-PRS-ChR2-mCherry). LC microinjection...
The mechanism by which ZIKV causes a range of neurological complications, especially congenital microcephaly, is not well understood. fact that microcephaly associated with Asian lineage strains raises the question why this was discovered earlier. One possible explanation and African differ in their abilities to infect cells CNS cause neurodevelopmental problems. Here, we show induce cell death human neural progenitor cells—which are important target development microcephaly—less efficiently...
West Nile virus (WNV) and Usutu (USUV) are closely related mosquito-borne neurotropic flaviviruses that share common transmission cycle can infect humans. However, while human infections by WNV widespread, USUV comparatively less frequent, severe, currently limited to Africa Europe. To identify host factors contribute the pathogenic signatures of these two flaviviruses, we carried out an arrayed expression screen over 1,300 interferon-stimulated genes (ISGs). Several ISGs known target...
Axonal transport is responsible for the movement of signals and cargo between nerve termini cell bodies. Pathogens also exploit this pathway to enter exit central nervous system. In study, we characterised binding, endocytosis axonal an adenovirus (CAV-2) that preferentially infects neurons. Using biochemical, biology, genetic, ultrastructural live-cell imaging approaches, show interaction with neuronal membrane correlates coxsackievirus receptor (CAR) surface expression, followed by...
Adenovirus vectors have significant potential for long- or short-term gene transfer. Preclinical and clinical studies using human derived adenoviruses (HAd) demonstrated the feasibility of flexible hybrid vector designs, robust expression induction protective immunity. However, use HAd can, under some conditions, be limited by pre-existing Pre-existing humoral cellular anti-capsid immunity limits efficacy duration transgene is poorly circumvented injections larger doses immuno-suppressing...
Environmental factors such as chemicals, stress and pathogens are now widely believed to play important roles in the onset of some brain diseases, they associated with neuronal impairment acute or chronic inflammation. Alzheimer's disease (AD) is characterized by progressive synaptic dysfunction neurodegeneration that ultimately lead dementia. Neuroinflammation also plays a prominent role AD possible links viruses have been proposed. In particular, human immunodeficiency virus (HIV) can pass...
The blood-brain barrier (BBB) largely prevents toxins and pathogens from accessing the brain. Some viruses have ability to cross this replicate in central nervous system (CNS). Zika virus (ZIKV) was responsible 2015 2016 for a major epidemic South America associated some cases with neurological impairments. Here, we characterized of mechanisms behind its neuroinvasion using an innovative vitro human BBB model. ZIKV efficiently replicated, released on parenchyma side, triggered subtle...
Background West Nile virus (WNV) and Usutu (USUV), two closely related flaviviruses, mainly follow an enzootic cycle involving mosquitoes birds, but also infect humans other mammals. Since 2010, their epidemiological situation may have shifted from irregular epidemics to endemicity in several European regions; this requires confirmation, as it could implications for risk assessment surveillance strategies. Aim To explore the seroprevalence animals potential of WNV USUV Southern France, given...
The transcription factor Elk-1 is a nuclear target of mitogen-activated protein kinases and regulates immediate early gene activation by extracellular signals. We show that also conjugated to SUMO on either lysines 230, 249, or 254. Mutation all three sites necessary fully block SUMOylation in vitro vivo. This mutant, Elk-1(3R), shuttles more rapidly nuclei Balb/C cells fused transfected HeLa cells. Coexpression SUMO-1 -2 strongly reduces shuttling without affecting indicating retention...
Various neurological symptoms have been associated to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection including headache, fever, anosmia, ageusia, but also, encephalitis, Guillain-Barre and ischemic stroke. Responsible for the current disease (COVID-19) pandemic, SARS-CoV-2 may access affect central nervous system (CNS) by several pathways such as axonal retrograde transport or through interaction with blood-brain barrier (BBB) blood-cerebrospinal fluid (CSF) barrier....
Among emerging arthropod-borne viruses (arbovirus), West Nile virus (WNV) is a flavivirus that can be associated with severe neuroinvasive infections in humans. In 2018, the European WNV epidemic resulted over 2000 cases, representing most important arboviral continent. Characterization of inflammation and neuronal biomarkers released during infection, especially context impairments, could provide insight into development predictive tools beneficial for patient outcomes. We first analyzed...
In the last decade, number of emerging Flaviviruses described worldwide has increased considerably. Among them Zika virus (ZIKV) and Usutu (USUV) are African mosquito-borne viruses that recently emerged. Recently, ZIKV been intensely studied due to major outbreaks associated with neonatal death birth defects, as well neurological symptoms. USUV pathogenesis remains largely unexplored, despite significant human veterinary disorders. Circulation in Africa was documented more than 50 years ago,...
Zika virus (ZIKV) has recently re-emerged as a pathogenic agent with epidemic capacities was well illustrated in South America. Because of the extent this health crisis, number more serious symptoms have become associated ZIKV infection than what initially described. In particular, neuronal and ocular disorders been characterized, both infants adults. Notably, macula retina can be strongly affected by ZIKV, possibly direct effect virus. This is supported detection replicative infectious...