A5061881016- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Bacteriophages and microbial interactions
- Insect symbiosis and bacterial influences
- Plant Parasitism and Resistance
- Animal Virus Infections Studies
- Evolution and Genetic Dynamics
- Insect Resistance and Genetics
- Malaria Research and Control
- Viral Infectious Diseases and Gene Expression in Insects
- Transgenic Plants and Applications
- Entomopathogenic Microorganisms in Pest Control
- Plant and Fungal Interactions Research
- Viral Infections and Outbreaks Research
- CRISPR and Genetic Engineering
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Plant Disease Resistance and Genetics
- Dengue and Mosquito Control Research
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Vector-borne infectious diseases
- Legume Nitrogen Fixing Symbiosis
- Zoonotic diseases and public health
Animal, Santé, Territoires, Risques et Ecosystèmes
2018-2024
Université de Montpellier
2002-2024
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2014-2024
Centre Hospitalier Universitaire de Montpellier
2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2024
COMUE Languedoc-Roussillon Universités
2023
Biologie et Génétique des Interactions Plante-Parasite
2009-2019
Institut Agro Montpellier
2013-2019
Agropolis International
2012-2019
Centre de Recherche et de Veille sur les Maladies Emergentes dans l’Océan Indien
2016
Abstract Infection with Usutu virus (USUV) has been recently associated neurologic disorders, such as encephalitis or meningoencephalitis, in humans. These findings indicate that USUV is a potential health threat. We report an acute human infection France putatively clinical diagnosis of idiopathic facial paralysis.
Genome segmentation is mainly thought to facilitate reassortment. Here, we show that can also allow differences in segment abundance populations of bluetongue virus (BTV). BTV has a genome consisting 10 segments, and its cycle primarily involves periodic alternation between ruminants Culicoides biting midges. We have developed reverse transcription-quantitative PCR (RT-qPCR) approach quantify each wild sampled both midges during an epizootic. Segment frequencies deviated from equimolarity...
During plaque purification of Spodoptera littoralis nucleopolyhedrovirus in S. Sl52 cell culture, a deletion mutant virus was isolated. Analysis the biological properties this revealed an absence per os infectivity occluded virus. Infectivity by injection non-occluded (budded) is not different between wild-type and deleted Restriction analysis genome 4·5 kb within Not I D fragment. The observed phenotype mapped to region rescue experiments. characterized equivalent DNA fragment on sequenced....
Summary Transmission from host to is a crucial step in the life cycle of pathogens, particularly viruses, ensuring spread and maintenance populations. The immobile nature plants strong pectin cellulose barrier surrounding cells have constrained most plant virus species use vectors (mainly insects) for exit, transfer entry one another. A growing body evidence showing that viruses can influence vector physiology behaviour increase their chances transmission, either directly or through...
A founding paradigm in virology is that the spatial unit of viral replication cycle an individual cell. Multipartite viruses have a segmented genome where each segment encapsidated separately. In this situation not recapitulated single virus particle but population. How multipartite manage to efficiently infect cells with all segments, thus whole information, long-standing perhaps deceptive mystery. By localizing and quantifying segments nanovirus host plant tissues we show they rarely...
Background West Nile virus (WNV) and Usutu (USUV), two closely related flaviviruses, mainly follow an enzootic cycle involving mosquitoes birds, but also infect humans other mammals. Since 2010, their epidemiological situation may have shifted from irregular epidemics to endemicity in several European regions; this requires confirmation, as it could implications for risk assessment surveillance strategies. Aim To explore the seroprevalence animals potential of WNV USUV Southern France, given...
Improved RNA virus understanding is critical to studying animal and plant health, environmental processes. However, the continuous rapid evolution makes their identification characterization challenging. While recent sequence-based advances have led extensive discovery, there growing variation in how viruses are identified, analyzed, characterized, reported. To this end, an RdRp Summit was organized a hybrid meeting took place Valencia, Spain May 2023 convene leading experts with emphasis on...
Recombination, complementation and competition profoundly influence virus evolution epidemiology. Since viruses are intracellular parasites, the basic parameter determining potential for such interactions is multiplicity of cellular infection (cellular MOI), i.e. number viral genome units that effectively infect a cell. The MOI values prevail in host organisms have rarely been investigated, whether they remain constant or change widely during invasion totally unknown. Here, we fill this...
ABSTRACT Plant virus species of the family Nanoviridae have segmented genomes with highest known number segments encapsidated individually. They thus likely represent most extreme case so-called multipartite, or multicomponent, viruses. All are believed to be transmitted in a circulative nonpropagative manner by aphid vectors, meaning that simply crosses cellular barriers within body, from gut salivary glands, without replicating even expressing any its genes. However, this assumption is...
The multiplicity of cellular infection (MOI) is the number virus genomes a given species that infect individual cells. This parameter chiefly impacts severity within-host population bottlenecks as well intensity genetic exchange, competition, and complementation among viral genotypes. Only few formal estimations MOI currently are available, most theoretical reports have considered this constant within infected host. Nevertheless, colonization multicellular host complex process during which...
For any organism, population size, and fluctuations thereof, are of primary importance in determining the forces driving its evolution. This is particularly true for viruses—rapidly evolving entities that form populations with transient explosive expansions alternating phases migration, resulting strong bottlenecks associated founder effects increase genetic drift. A typical illustration this pattern progression viral disease within a eukaryotic host, where such demographic key factor...
West Nile virus (WNV) and Usutu (USUV) are zoonotic arboviruses. These flaviviruses mainly maintained in the environment through an enzootic cycle involving mosquitoes birds. Horses humans incidental, dead-end hosts, but can develop severe neurological disorders. Nevertheless, there is little data regarding involvement of other mammals epidemiology these In this study, we performed a serosurvey to assess exposure viruses captive birds zoo situated south France, area described for circulation...
Abstract Background Mosquitoes (Diptera: Culicidae) can have a significant negative impact on human health. The urbanization of natural environments and their conversion for agricultural use, as well population growth, may affect mosquito populations increase the risk emerging or re-emerging mosquito-borne diseases. We report variety number adult mosquitoes found in four with varying degrees (rural, urban, rice fields, forest) located savannah zone West Africa. Methods were collected from...
Human encephalitis represents a medical challenge from diagnostic and therapeutic point of view. We investigated the cause 2 fatal cases unknown origin in immunocompromised patients.Untargeted metatranscriptomics was applied on brain tissue patients to search for pathogens (viruses, bacteria, fungi, or protozoans) without prior hypothesis.Umbre arbovirus, an orthobunyavirus never previously identified humans, found patients. In situ hybridization reverse transcriptase-quantitative polymerase...
Abstract Our knowledge of the diversity eukaryotic viruses has recently undergone a massive expansion. This could influence host physiology through yet unknown phenomena potential interest to fields health and food production. However, assembly processes this remain elusive in viromes terrestrial animals. situation hinders hypothesis-driven tests virome on physiology. Here, we compare taxonomic between different spatial scales mosquito Culex pipiens. is vector human pathogens worldwide. The...
Tomato yellow leaf curl virus (TYLCV) is a highly damaging begomovirus native to the Middle East. TYLCV has recently spread worldwide, recombining with other begomoviruses. Recent analysis of mixed infections between and Comoros (ToLCKMV) shown that, although natural selection preserves certain co-evolved intra-genomic interactions, numerous diverse recombinants are produced at 120 days post-inoculation (dpi), recombinant populations from different tomato plants very divergent. Here, we...
Abstract Mosquito-borne viruses represent a threat to human health worldwide. This taxonomically-diverse group includes numerous that recurrently spread into new regions. Thus, periodic surveillance of the arbovirus diversity in given region can help optimizing diagnosis arboviral infections. Nevertheless, such screenings are rarely carried out, especially low-income countries. Consequently, case investigation is often limited fraction diversity. situation probably results undiagnosed cases....
Usutu virus (USUV) and West Nile (WNV) are phylogenetically closely related arboviruses. These viruses mainly follow an enzootic cycle involving mosquitoes birds, but they occasionally infect humans other mammals, inducing neurotropic disorders. Since the discovery of USUV, only two human cases have been reported in Africa, including one Burkina Faso 2004. then, no studies conducted to measure extent circulation this Faso, study regarding WNV has conducted. Our aimed determine seroprevalence...