A5044422060- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Entomopathogenic Microorganisms in Pest Control
- Plant Virus Research Studies
- Insect Pest Control Strategies
- Insect and Pesticide Research
- Insect-Plant Interactions and Control
- Nematode management and characterization studies
- Forest Insect Ecology and Management
- Sustainable Industrial Ecology
- Insect and Arachnid Ecology and Behavior
- Insect behavior and control techniques
- French Urban and Social Studies
- Insect symbiosis and bacterial influences
- Virus-based gene therapy research
- Insect Utilization and Effects
- Viral gastroenteritis research and epidemiology
- Wastewater Treatment and Reuse
- Insects and Parasite Interactions
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Date Palm Research Studies
- Mollusks and Parasites Studies
- Plant Pathogens and Resistance
- Animal Virus Infections Studies
IMT Mines Alès
2015-2025
Centre National de la Recherche Scientifique
1998-2025
Institut de Recherche pour le Développement
2021-2025
Laboratoire HydroSciences Montpellier
2021-2024
Université de Montpellier
2001-2023
Territoires
2020-2021
Laboratoire Génie Industriel
2011-2020
Institut Mines-Télécom
2014-2020
ELSA
2018
Télécom Paris
2016
During plaque purification of Spodoptera littoralis nucleopolyhedrovirus in S. Sl52 cell culture, a deletion mutant virus was isolated. Analysis the biological properties this revealed an absence per os infectivity occluded virus. Infectivity by injection non-occluded (budded) is not different between wild-type and deleted Restriction analysis genome 4·5 kb within Not I D fragment. The observed phenotype mapped to region rescue experiments. characterized equivalent DNA fragment on sequenced....
Cydia pomonella granulovirus (CpGV) has been used for 15 years as a bioinsecticide in codling moth (Cydia pomonella) control. In 2004, some insect populations with low susceptibility to the virus were detected first time southeast France. RGV, laboratory colony of moths resistant CpGV-M isolate field, was established collection insects field followed by an introgression trait into susceptible (Sv). The resistance level (based on 50% lethal concentrations [LC(50)s]) RGV isolate, active...
Defective viruses lack genes essential for survival but they can co-infect with complete virus genotypes and use gene products from the genotype their replication transmission. As such, are detrimental to fitness of genotypes. Here, we describe a mutualistic interaction between an insect baculovirus (nucleopolyhedrovirus Spodoptera frugiperda (Lepidoptera)) that increases pathogenicity viral population. Mixtures able be transmitted orally deletion mutant unable resulted in phenotype...
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The Lepidoptera Spodoptera frugiperda is a pest which causes widespread economic damage on variety of crop plants. It also well known through its famous Sf9 cell line used for numerous heterologous protein productions. Species the genus are as model pesticide resistance and to study virus host interactions. A genomic approach now critical step further new developments in biology pathology these insects, results ESTs sequencing efforts need be structured into databases providing an integrated...
ABSTRACT A Nicaraguan field isolate (SfNIC) of Spodoptera frugiperda nucleopolyhedrovirus was purified by plaque assay on Sf9 cells. Nine distinct genotypes, to I, were identified their restriction endonuclease profiles. Variant SfNIC-B selected as the standard because its profile corresponded that wild-type isolate. Physical maps generated for each variants. The differences between variants and confined region map units 9 32.5. This included PstI-G, PstI-F, PstI-L, PstI-K EcoRI-L fragments....
An insect nucleopolyhedrovirus naturally survives as a mixture of at least nine genotypes. Infection by multiple genotypes results in the production virus occlusion bodies (OBs) with greater pathogenicity than those any genotype alone. We tested hypothesis that each OB contains genotypically diverse population virions. Few insects died following inoculation an experimental two-genotype dose one per insect, but high proportion infections were observed (50%), which differed significantly from...
Autographa californica nucleopolyhedrovirus (Ac- MNPV) does not replicate in Bombyx mori cells (Bm5, BmN). We have shown previously that when a short DNA sequence within AcMNPV ORF95, which encodes the viral helicase P143, is replaced with collinear region of B. (BmNPV), gains ability to Bm5 cells. To determine mutational events p143 gene required allow replication cells, recombinants produced Sf9 were screened vivo larvae, are more permissive baculo- virus infection than cell lines. Eight...
Defective viruses, that are deficient in certain essential genes, maintained the population by trans-complementation, exploiting gene products of complete genotypes co-infected cells. This process becomes prevalent only when cells frequently infected several virus particles, and then will fitness defective viruses be subjected to frequency-dependent selection. Deletion variants not infectious per os present a multicapsid nucleopolyhedrovirus (SfMNPV, Baculoviridae) infects fall army worm,...
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ABSTRACT A Nicaraguan isolate of a nucleopolyhedrovirus (SfNIC) that attacks the fall armyworm, Spodoptera frugiperda , survives as mixture nine genotypes (SfNIC to I) all present genomic deletions, except variant B (complete genotype). Sequencing cloned restriction fragments revealed genotypic variants lack between 5 and 16 open reading frames in contiguous sequence 18 kb SfNIC genome. The absence oral infectivity SfNIC-C -D is related deletion pif and/or pif-2 gene, while SfNIC-G remains...
The aim of this study is to investigate the potential ultraviolet/visible (UV/Vis) spectrometry as a complementary method for routine monitoring reclaimed water production. Robustness models and compliance their sensitivity with current quality limits are investigated. following indicators studied: total suspended solids (TSS), turbidity, chemical oxygen demand (COD) nitrate. Partial least squares regression (PLSR) used find linear correlations between absorbances interest. Artificial...
Superinfection exclusion is the ability of an established virus to interfere with a second infection. This effect was studied in vitro during lepidopteran-specific nucleopolyhedrovirus (genus Alphabaculovirus, family Baculoviridae) Homologous interference detected Sf9 cells sequentially infected two genotypes Autographa californica multiple (AcMNPV), each one expressing different fluorescent protein. progressive process which sharp decrease signs infection caused by observed, affecting not...