A5032614721- RNA and protein synthesis mechanisms
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- RNA modifications and cancer
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- Insect-Plant Interactions and Control
- Environmental Toxicology and Ecotoxicology
- Insect Utilization and Effects
- Plant and Fungal Interactions Research
- Circular RNAs in diseases
- Genomics and Phylogenetic Studies
- Viral Infections and Immunology Research
- Insect and Pesticide Research
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Pesticide Exposure and Toxicity
- Nematode management and characterization studies
- Plant Physiology and Cultivation Studies
- Neurobiology and Insect Physiology Research
- Advanced biosensing and bioanalysis techniques
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Hepatitis B Virus Studies
- Plant Disease Resistance and Genetics
Universitat Politècnica de València
2017-2025
Instituto de Biología Molecular y Celular de Plantas
2014-2024
Consejo Superior de Investigaciones Científicas
2017-2024
University of Puerto Rico at Carolina
2024
Universitat de València
1999-2011
Association for Computing Machinery
2006
Earth's life may have originated as self-replicating RNA, and it has been argued that RNA viruses viroid-like elements are remnants of such pre-cellular world. defined by linear genomes encoding an RNA-dependent polymerase (RdRp), whereas consist small, single-stranded, circular that, in some cases, encode paired self-cleaving ribozymes. Here we show the number candidate occurring geographically ecologically diverse niches is much higher than previously thought. We report amongst these...
Improved RNA virus understanding is critical to studying animal and plant health, environmental processes. However, the continuous rapid evolution makes their identification characterization challenging. While recent sequence-based advances have led extensive discovery, there growing variation in how viruses are identified, analyzed, characterized, reported. To this end, an RdRp Summit was organized a hybrid meeting took place Valencia, Spain May 2023 convene leading experts with emphasis on...
Catalytic RNAs, or ribozymes, are regarded as fossils of a prebiotic RNA world that have remained in the genomes modern organisms. The simplest ribozymes small self-cleaving like hammerhead ribozyme, which been historically considered biological oddities restricted to some pathogens. Recent data, however, indicate widespread genomes, although their functions still unknown.We reveal ribozyme sequences plant form part new family non-autonomous retrotransposons with referred retrozymes. These...
Small self-cleaving RNAs, such as the paradigmatic Hammerhead ribozyme (HHR), have been recently found widespread in DNA genomes across all kingdoms of life. In this work, we that new HHR variants are preserved ancient family Penelope-like elements (PLEs), a group eukaryotic retrotransposons regarded exceptional for encoding telomerase-like retrotranscriptases and spliceosomal introns. Our bioinformatic analysis revealed not only presence minimalist HHRs two flanking repeats PLEs but also...
Resistance to malathion has been reported in field populations of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), areas Spain where an intensive use this insecticide was maintained for several years. The main goal study determine whether resistance confers cross-resistance different types insecticides. Susceptibility bioassays showed that malathion-resistant W-4Km strain (176-fold more resistant than susceptible C strain) moderate levels (three- 16-fold)...
Ribozymes are catalytic RNAs present in modern genomes but regarded as remnants of a prebiotic RNA world. The paradigmatic hammerhead ribozyme (HHR) is small self-cleaving motif widespread from bacterial to human genomes. Here, we report that most the classical type I HHRs frequently found animals contained within novel family non-autonomous non-LTR retrotransposons retrozyme class. These retroelements expressed abundant linear and circular ∼170-400 nt different animal tissues. Bioinformatic...
Human hepatitis delta virus (HDV) is a unique infectious agent whose genome composed of small circular RNA. Recent data, however, have reported the existence highly divergent HDV-like circRNAs in transcriptomes diverse vertebrate and invertebrate species. The genomes described amniotes such as birds reptiles encode self-cleaving RNA motifs or ribozymes similar to ones present human HDV, whereas no catalytic domains been for detected metagenomic data from some amphibians, fish, invertebrates....
A new family of non-autonomous retrotransposons with self-cleaving hammerhead ribozymes, the so called retrozymes, has recently been found encoded in diverse plant genomes. These retroelements can be actively transcribed, and their RNAs accumulate cells as abundant non-coding circular (circRNAs) small size (600-1000 nt). Related circRNAs ribozymes had already described plants, belong to a group infectious RNA agents an uncertain origin: viroids viroid-like satellites viruses. pathogenic show...
Abstract The western flower thrips Frankliniella occidentalis (Pergande) is a very significant pest of number different agricultural crops in the south‐east Spain. importance as not due mainly to direct damage inflicted on plant, but loss commercial value which occurs consequence development dark spots caused by tomato spotted wilt virus (TSWV) they transmit. economic threshold therefore almost zero, enhances problems resistance management. present work part global project that attempts...
Catalase (CAT), glutathione reductase (GR), S-transferase (GST), and thiobarbituric acid reactive substances (TBARS) were measured together with cadmium (Cd) toxicity accumulation in newly molted adults of Oncopeltus fasciatus (Dallas) exposed to the metal drinking water. Insects provided either 200 or 20 mg Cd/liter (96-h LC30 LC10, respectively) tap water for 1, 3, 5, 7 d. concentration accumulated Cd a time-dependent manner. Peroxilipids as TBARS significantly elevated (1.7- 1.9-fold)...
Abstract Small self-cleaving ribozymes are catalytic RNAs originally discovered in viroid-like agents, which infectious circular (circRNAs) postulated as relics of a prebiotic RNA world. In the last decade, however, small have also been detected across tree life, from bacterial to human genomes, and more recently, viral agents with circRNA genomes. Here we report conserved occurrence within linear genomes typical ds ssRNA viruses fungi plants. most 5’-UTR regions chrysovirids fusarivirids,...
Abstract The western flower thrips, Frankliniella occidentalis (Pergande), is a serious pest in the south‐east of Spain owing to its direct feeding on crops, transmission tomato spotted wilt virus and very high level resistance insecticides. Mechanisms were examined using field populations F. with different susceptibilities acrinathrin, methiocarb (selective insecticides), endosulfan, metamidophos deltamethrin (broad‐spectrum insecticides). Esterase activity towards α‐naphthyl acetate p...
Abstract BACKGROUND: Frankliniella occidentalis (Pergande) is among the most important crop pests in south‐east region of Spain; its increasing resistance to insecticides constitutes a serious problem, and understanding mechanisms involved therefore great interest. To this end, F. populations, collected from field at different locations Spain, were studied terms total esterase activity isoenzyme pattern. RESULTS: Individual thrips extracts analysed by native polyacrylamide gel...
Abstract Earth’s life may have originated as self-replicating RNA. Some of the simplest current RNA replicators are viruses, defined by linear genomes encoding an RNA-dependent polymerase (RdRP), and subviral agents with single-stranded, circular genomes, such viroids paired self-cleaving ribozymes. Amongst a massive expansion candidate viroid viroid-like elements, we report that fungal pathogens, ambiviruses, elements which undergo rolling circle replication encode their own viral RdRP,...
Abstract BACKGROUND: Western flower thrips (WFT), Frankliniella occidentalis (Pergande), is among the most important crop pests in south‐eastern region of Spain. Its increasing resistance to insecticides constitutes a serious problem, and understanding mechanisms involved therefore great interest. Use synergists inhibit enzymes insecticide detoxification widely used determine their responsibility for resistance. However, they do not always act as intended or expected, caution must be...
Circular and linear retrozyme RNAs in J. curcas F. ananassa. A: Double PAGE analysis of an RNA extract (~20 μg) from leaves. A gel stripe a 5 % native polyacrylamide containing 600–900 nt (left) was cut out run on top second denaturing (center). The corresponding Northern blot (right), using digoxigenin-labelled fragment as probe, revealed the presence both circular forms. B: extracts (~30 μg each) leaves, young seedlings seeds. Samples were detected same probe panel A. C: An vitro...