A5027489455- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Animal Virus Infections Studies
- Plant Pathogenic Bacteria Studies
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- Plant and Fungal Interactions Research
- Aquaculture disease management and microbiota
- RNA and protein synthesis mechanisms
- Microbial Community Ecology and Physiology
- SARS-CoV-2 detection and testing
- Virus-based gene therapy research
- Plant Disease Resistance and Genetics
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- Viral gastroenteritis research and epidemiology
- Botanical Research and Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Genetic diversity and population structure
- Plant Disease Management Techniques
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
Latvian Biomedical Research and Study Centre
2019-2024
Improved RNA virus understanding is critical to studying animal and plant health, environmental processes. However, the continuous rapid evolution makes their identification characterization challenging. While recent sequence-based advances have led extensive discovery, there growing variation in how viruses are identified, analyzed, characterized, reported. To this end, an RdRp Summit was organized a hybrid meeting took place Valencia, Spain May 2023 convene leading experts with emphasis on...
Rapid spread of antibiotic resistance throughout the kingdom bacteria is inevitably bringing humanity towards “post-antibiotic” era. The emergence so-called “superbugs”—pathogen strains that develop to multiple conventional antibiotics—is urging researchers around globe work on development or perfecting alternative means tackling pathogenic infections. Although various conceptually different approaches are being considered, each comes with its advantages and drawbacks. While drug-resistant...
The first complete genome that was sequenced at the beginning of sequencing era a phage, since then researchers throughout world have been steadily describing and publishing genomes from wide array phages, uncovering secrets most abundant diverse biological entities known to man. Currently, we are experiencing an unprecedented rate novel bacteriophage discovery, which can be seen fact amount entries in public sequence repositories has more than doubled past 3 years is growing without showing...
Pseudomonas syringae is a bacterial pathogen that causes yield losses in various economically important plant species. At the same time, P. pv. tomato (Pst) one of best-studied phytopathogens and popular model organism. In this study, we report on isolation two phages from market-bought pepper fruit showing symptoms speck. These were named Eir4 Eisa9 characterized using traditional microbiological methods whole-genome sequencing followed by bioinformatics approaches. Both isolated capable...
Abstract Endolysins are bacteriophage-encoded peptidoglycan-degrading enzymes with potential applications for treatment of multidrug-resistant bacterial infections. Hafnia phage Enc34 encodes an unusual endolysin N-terminal enzymatically active domain and a C-terminal transmembrane domain. The catalytic the belongs to conserved protein family PHA02564 which has no recognizable sequence similarity other known types. Turbidity reduction assays indicate that enzyme is against peptidoglycan from...
Remaining a major healthcare concern with nearly 29 million confirmed cases worldwide at the time of writing, novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused more than 920 thousand deaths since its outbreak in China, December 2019. First case person testing positive for SARS-CoV-2 infection within territory Republic Latvia was registered on 2nd March 2020, 9 days prior to pandemic declaration by WHO. Since then, 277,000 tests were carried out confirming total...
Representatives of the bacterial genus Aeromonas are some most notorious aquaculture pathogens associated with a range diseases in different fish species. As world forges toward post-antibiotic era, alternative options for combating needed. One such option is phage biocontrol. In this study, novel podophage—JELG-KS1—infecting salmonicida was retrieved from wastewater along its host strain. The genome JELG-KS1 40,505 bp dsDNA molecule GC% 53.42% and 185 direct terminal repeats encodes 53...
Pseudomonas aeruginosa is a bacteria responsible for many hospital-acquired infections. Phages are promising alternatives treating P. infections, which often intrinsically resistant. The combination of phage and antibiotics in clearing bacterial infection holds promise due to increasing reports enhanced effectiveness when both used together. aim the study isolate characterize novel determine its vitro with controlling aeruginosa. In this study, jumbo myophage HPP-Temi infecting Pa9...
The bacterial genus Pantoea comprises species found in a variety of different environmental sources. spp. are often recovered from plant material and capable both benefitting the plants acting like phytopathogens. Some (including P. agglomerans) considered opportunistic human pathogens causing various infections immunocompromised subjects. In this study, strain agglomerans (identified by 16S rRNA gene sequencing) was isolated dead specimen an unidentified Latvian grasshopper species....
Prophages or prophage remnants are found in chromosomes of many bacterial strains and might increase the environmental fitness and/or virulence their hosts. Up to this date complete genome sequences only seven temperate bacteriophages infecting bacteria from genus Erwinia, comprising mostly phytopathogenic bacteria, available publicly. No attempts analyze global diversity Erwinia phages establish relationships between cultured prophages were yet made. In study we have isolated, sequenced...
While looking for novel insect-associated phages, a unique siphophage, Nocturne116, was isolated from deceased local moth specimen along with its host, which identified by 16S rRNA gene sequencing as strain of Lactococcus lactis. Next-generation and the subsequent genome annotation elaborated on herein revealed that Nocturne116 is 25,554 bp long dsDNA molecule 10 3′ cos overhangs GC content 37.99%, comprising 52 predicted open reading frames. The complete nucleotide sequence phage dissimilar...
Both recognized species from the genus Morganella (M. morganii and M. psychrotolerans) are Gram-negative facultative anaerobic rod-shaped bacteria that have been documented as sometimes being implicated in human disease. Complete genomes of seven Morganella-infecting phages publicly available today. Here, we report on genomic characterization an insect associated sp. phage, which named Mecenats66, isolated dead worker honeybees. Phage Mecenats66 was propagated, purified, subjected to...
Abstract Endolysins are bacteriophage-encoded peptidoglycan-degrading enzymes with potential applications for treating multidrug-resistant bacterial infections. While exogenously applied endolysins active against Gram-positive bacteria in their native form, Gram-negative protected from such activity of most by an outer membrane. However, it was shown that recombinant can be designed to efficiently lyse without as well. During our previous efforts, we purified and structurally characterized...
Abstract Background The causative agent of the COVID‐19 pandemic, severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2), is zoonotic origin and has shown reverse transmissibility. Objectives aim this cross‐sectional study was to investigate serological molecular prevalence SARS‐CoV‐2 infection in domestic cat ( Felis catus ) population from Latvia natural conditions subsequently perform viral genome analysis. Methods Oropharyngeal rectal swabs blood samples were collected 273 cats...
The agricultural importance of sea buckthorn (SBT; Hippophae rhamnoides L.) is rapidly increasing. Several bacterial and fungal pathogens infecting SBT have been identified characterized; however, the viral are not yet known. In this study, we identified, isolated, sequenced a virus from wild plantation for first time. Sequence analysis obtained genome revealed high similarity with several viruses belonging to genus Marafivirus. new 6989 nucleotides (nt) in length according 5', 3' RACE...
Abstract Remaining a major healthcare concern with nearly 29 million confirmed cases worldwide at the time of writing, novel severe acute respiratory syndrome coronavirus - 2 (SARS-CoV-2) has caused more than 920 thousand deaths since its outbreak in China, December 2019. First case person testing positive for SARS-CoV-2 infection within territory Republic Latvia was registered on nd March 2020, nine days prior to pandemic declaration by WHO. Since then, 277 000 tests were carried out...
In a recent study published in Viruses [...]
Abstract The agricultural importance of sea buckthorn ( Hippophae rhamnoides L.) is rapidly increasing. Several bacterial and fungal pathogens infecting have been identified characterized; however, the viral are not yet known. In this study, we identified, isolated, sequenced a virus from wild plantation for first time. Sequence analysis obtained genome revealed high similarity with sequences several viruses belonging to genus Marafivirus , especially olive latent 3 (OLV-3). new 6,989...