A5025553086- Aquaculture disease management and microbiota
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- Aquaculture Nutrition and Growth
- Vibrio bacteria research studies
- Streptococcal Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Actinomycetales infections and treatment
- Chromosomal and Genetic Variations
- Neonatal and Maternal Infections
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Genetic diversity and population structure
- Probiotics and Fermented Foods
- Plant Virus Research Studies
- Salmonella and Campylobacter epidemiology
- Molecular Biology Techniques and Applications
- Microbial Metabolism and Applications
- Invertebrate Immune Response Mechanisms
- Escherichia coli research studies
- Zebrafish Biomedical Research Applications
- Reproductive biology and impacts on aquatic species
- Parasitic Infections and Diagnostics
University of Miyazaki
2009-2024
Japan Fisheries Research and Education Agency
2015-2020
Nocardiosis caused by Nocardia seriolae is one of the major threats in aquaculture Seriola species (yellowtail; S. quinqueradiata, amberjack; dumerili and kingfish; lalandi) Japan. Here, we report complete nucleotide genome sequence N. UTF1, isolated from a cultured yellowtail. The circular chromosome 8,121,733 bp with G+C content 68.1% that encodes 7,697 predicted proteins. In UTF1 genes, found orthologs virulence factors pathogenic mycobacteria human clinical isolates involved host cell...
Abstract The Pacific bluefin tuna, Thunnus orientalis , is a highly migratory species that widely distributed in the North Ocean. Like other marine species, T. has no external sexual dimorphism; thus, identifying sex-specific variants from whole genome sequence data useful approach to develop an effective sex identification method. Here, we report improved draft of and male-specific DNA markers. Combining PacBio long reads Illumina short sufficiently assembly, with 38-fold increase scaffold...
Abstract A novel Asfarvirus-like virus is proposed as the etiological agent responsible for mass mortality in abalone. The disease, called abalone amyotrophia, originally was recognized 1980s, but efforts to identify a causative were unsuccessful. We prepared semi-purified fraction by nuclease treatment and ultracentrifugation of diseased homogenate, existence confirmed challenge test. Using next-generation sequencing PCR-based epidemiological surveys, we obtained partial sequence with...
Seriola quinqueradiata (yellowtail) is the most widely farmed and economically important fish in aquaculture Japan. In this study, we used genome of haploid yellowtail larvae for de novo assembly whole-genome sequences, built a high-quality draft yellowtail. The total length assembled sequences was 627.3 Mb, consisting 1,394 scaffold (>2 kb) with an N50 1.43 Mb. A 27,693 protein-coding genes were predicted genome, among these, 25,832 (93.3%) functionally annotated. Given our lack knowledge...
In bluefin tuna aquaculture, high mortalities of hatchery-reared juveniles occur in sea cages owing to wall collisions that are caused by high-speed swimming panic due changes illuminance. Here, we report targeted gene mutagenesis the ryanodine receptor (RyR1b), which allows sarcoplasmic reticulum release Ca2+ fast skeletal muscle, using highly active Platinum TALENs slow behaviour response external stimuli Pacific (PBT) larvae. This characteristic would be a useful trait prevent aquaculture...
The newly emerging pathogen Lactococcus garvieae serotype II, which appears to be morphologically and biochemically indistinguishable from L. I, has been isolated farmed Seriola quinqueradiata S. dumerili. To distinguish the two serotypes, a primer set was designed target glxR-argS intergenic region. PCR with amplified different-sized fragments: 1,285 bp for II 285 I. Therefore, enables PCR-mediated identification of strains marine fish species in Japan.
Next-generation sequencing technologies have allowed the rapid determination of complete genomes many organisms. Although shotgun sequences from large genome organisms are still difficult to reconstruct perfect contigs each which represents a full chromosome, those small been assembled successfully into very number contigs. In this study, we show that reads phage can be reconstructed single contig by controlling read used in de novo assembly. We developed pipeline assemble viral with good...
Nonagglutinating Lactococcus garvieae has been isolated from diseased farmed yellowtail in Japan since 2012. In this study, the complete genome and plasmid sequence of nonagglutinating L. strain 122061 was determined, to our knowledge, for first time.
The Japanese eel (Anguilla japonica) is among the most important aquaculture fish species in Eastern Asia. present study aimed to identify genetic parameters underlying body size and timing at metamorphosis from leptocephali glass eels captive-bred eels, with intent foster sustainable development. Larvae a partly factorial cross (14 sires × 11 dams) were reared until point of into eels. In these organisms, we observed moderate heritability mild correlations traits related (h2 = 0.16–0.33)...
Lactococcus garvieae serotype II, a newly emerging causal pathogen of lactococcal infection, has spread to fish farms since 2012, and its damage aquaculture increased. A total 161 strains were isolated from four species, yellowtail Seriola quinqueradiata, amberjack S. dumerili, kingfish lalandi, striped jack Pseudocaranx dentex, in eight prefectures between 2012 2017. This is the first epidemiological study on L. by using agglutination titers against antisera for diagnosis, biased...
Lactococcus garvieae serotype III, a newly emerging pathogen that causes lactococcal infection, has been detected in fish farms since 2021. This caused severe damage to the striped jack Pseudocaranx dentex and greater amberjack Seriola dumerili. study collected strains from various species on between 2021 2023 conducted an epidemiological characterization of L. III using biochemical tests, agglutinating titers against diagnostic antisera, biased sinusoidal field gel electrophoresis (BSFGE),...
Bluefin tunas are one of the most important fishery resources worldwide. Because high market values, bluefin tuna farming has been rapidly growing during recent years. At present, common form is based on stocking wild-caught fish. Therefore, concerns have raised about negative impact wild stocks. Recently, Pacific (PBT), Thunnus orientalis, succeeded in completing reproduction cycle under aquaculture conditions, but production bottlenecks remain to be solved because very little biological...
Aims: Fish pathogenic Lactococcus garvieae serotype II has been isolated from cultured fish species in Japan. This study aimed to investigate the molecular mechanisms of lincomycin (LCM)-resistant L. and assess basis for lincosamides-streptogramins A-pleuromutilins (LSAP)-resistant phenotype. Results: We identified a novel lsa(D)-encoded 497-aa ATP-binding cassette F (ABC-F) protein LSAP-resistant strains. Amino acid identities 41.25-54.73% were obtained between deduced amino acids Lsa(D)...
Here, we report the complete genome sequence of a potentially new serotype, Lactococcus garvieae strain MS210922A, isolated from greater amberjack. It is nonreactive to serotype I or II antisera. The comprises 2,007,159 bp, including 1,929 coding, 16 rRNA, and 58 tRNA genes, with 38.9% G+C content.
Abstract Since 2011, high mortality rates and symptoms consistent with vibriosis have been observed in farmed amberjack ( Seriola dumerili ) Japan. To identify 41 strains isolated from diseased amberjack, a multilocus sequence analysis using nine concatenated genes ftsZ , gapA gyrB mreB pyrH recA rpoA topA 16S rRNA) was conducted. Twenty‐seven were identified as Vibrio harveyi suggesting an epidemic of V. infection farms. Other anguillarum owensii Photobacterium damselae subsp. . develop...
Recent improvements in next-generation sequencing technology have made it possible to do whole genome sequencing, on even non-model eukaryote species with no available reference genomes. However, de novo assembly of diploid genomes is still a big challenge because allelic variation. The aim this study was determine the feasibility utilizing haploid fish larvae for whole-genome sequences. We compared efficiency using yellowtail (Seriola quinqueradiata) that obtained from dam. De and sequence...
Lancefield group C Streptococcus dysgalactiae (GCSD) is known as a causative agent of bovine mastitis and cardiopulmonary diseases in humans. Recently, GCSD has been isolated from diseased fish Japan. Almost all culture supernatants sodium dodecyl sulfate extracts obtained farmed possessed serum opacity activity. Serum factor (SOF) bifunctional cell-associated protein that causes opacification. In this study, gene coding SOF, which was named sof-FD, identified fish. The amino acid sequence...