A5085159837- Birth, Development, and Health
- Epigenetics and DNA Methylation
- Genetic Syndromes and Imprinting
- Adipose Tissue and Metabolism
- Gestational Diabetes Research and Management
- Diet and metabolism studies
- Pregnancy and preeclampsia studies
- Nutrition, Genetics, and Disease
- Animal Genetics and Reproduction
- RNA modifications and cancer
- Italian Social Issues and Migration
- Educational and Social Studies
- Reproductive biology and impacts on aquatic species
- Prenatal Screening and Diagnostics
- Cancer-related gene regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Milk Quality and Mastitis in Dairy Cows
- Cancer-related molecular mechanisms research
- Regulation of Appetite and Obesity
- Neuroendocrine regulation and behavior
- Health, Environment, Cognitive Aging
- Sexual Differentiation and Disorders
- Service and Product Innovation
- Stress Responses and Cortisol
- Infant Nutrition and Health
Biologie de la Reproduction, Environnement, Epigénétique et Développement
2019-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2018-2024
École Nationale Vétérinaire d'Alfort
2010-2023
Université Paris-Saclay
2016-2023
Université de Versailles Saint-Quentin-en-Yvelines
2020-2023
Laboratoire de Biologie du Développement
2010-2019
Centre National de la Recherche Scientifique
2009-2016
Inserm
2009-2016
Institut Cochin
2005-2016
Université Paris Cité
2009-2016
The imprinted H19 gene produces a non-coding RNA of unknown function. Mice lacking show an overgrowth phenotype, due to cis effect the locus on adjacent Igf2 gene. To explore function itself, we produced transgenic mice overexpressing H19. We observed postnatal growth reduction in two independent lines and detected decrease Igf2expression embryos. An extensive analysis several other genes from newly described network (IGN) was performed both loss- gain-of-function animals. found that...
The H19 locus belongs to a cluster of imprinted genes that is linked the human Beckwith-Wiedemann syndrome. expression and its closely associated IGF2 gene frequently deregulated in some tumors, such as Wilms' tumors. In these cases, biallelic lack are with hypermethylation imprinting center this locus. These observations others have suggested potential tumor suppressor effect Some studies also an oncogene, based on tissue culture systems. We show, using vivo murine models tumorigenesis,...
Sex differences occur in most non-communicable diseases, including metabolic hypertension, cardiovascular disease, psychiatric and neurological disorders cancer. In many cases, the susceptibility to these diseases begins early development. The observed between sexes may result from genetic hormonal responses interactions with environmental factors, infection, diet, drugs stress. placenta plays a key role fetal growth development and, as such, affects programming underlying subsequent adult...
Background Changes in imprinted gene dosage the placenta may compromise prenatal control of nutritional resources. Indeed monoallelic behaviour and sensitivity to changes regional epigenetic state render genes both vulnerable adaptable. Methods Findings We investigated whether a high-fat diet (HFD) during pregnancy modified expression local global DNA methylation patterns placenta. Pregnant mice were fed HFD or (CD) first 15 days gestation. compared total homogenates, for male female...
Males and females responses to gestational overnutrition set the stage for subsequent sex-specific differences in adult onset non communicable diseases. Placenta, as a widely recognized programming agent, contibutes underlying processes. According our previous findings, high-fat diet during gestation triggers epigenetic alterations within CpG throughout genome, together with deregulation of clusters imprinted genes. We further investigated impact sex on placental histology, transcriptomic...
According to the Developmental Origin of Health and Disease (DOHaD) concept, alterations nutrient supply in fetus or neonate result long-term programming individual body weight (BW) setpoint. In particular, maternal obesity, excessive nutrition, accelerated growth neonates have been shown sensitize offspring obesity. The white adipose tissue may represent a prime target metabolic induced by order unravel underlying mechanisms, we developed rat model obesity using high-fat (HF) diet...
Maternal obesity impacts fetal growth and pregnancy outcomes. To counteract the deleterious effects of on fertility issue, preconceptional weight loss is recommended to obese women. Whether this beneficial/detrimental for offspring remains poorly explored. Epigenetic mechanisms could be affected by maternal changes, perturbing expression key developmental genes in placenta or fetus. Our aim was investigate chronic feto-placental along with underlying epigenetic mechanisms. We also tested...
The H19 locus controls fetal growth by regulating expression of several genes from the imprinted gene network (IGN). is fully repressed after birth, except in skeletal muscle. Using loss-of-function H19(Δ3) mice, we investigated function adult Mutant muscles display hypertrophy and hyperplasia, with increased Igf2 decreased myostatin (Mstn) expression. Many are expressed muscle stem cells or satellite cells. Unexpectedly, number was reduced 50% fibers. This reduction occurred postnatal day...
Genomic imprinting regulates the expression of a group genes monoallelically expressed in parent-of-origin specific manner. Allele-specific DNA methylation occurs at differentially methylated regions (DMRs) these genes. We have previously shown that vitro fertilization and embryo culture result defects imprinted H19-Igf2 locus blastocyst stage. The current study was designed to evaluate consequences manipulations on genomic after implantation mouse. Blastocysts were produced following three...
Low birth weight is associated with an increased risk for developing type 2 diabetes and metabolic diseases. The placental capacity to supply nutrients oxygen the fetus represents main determiner of fetal growth. However, few studies have investigated effects maternal diet on placenta. We explored adaptive proteomic processes implicated in response undernutrition. Rat term placentas from 70% food-restricted (FR30) mothers were used a screen. Placental mitochondrial functions evaluated using...
According to the developmental origins of health and diseases (DOHaD), in line with findings many studies, obesity during pregnancy is clearly a threat well-being offspring, later adulthood. We previously showed that 20% male female inbred mice can cope obesogenic effects high-fat diet (HFD) for 20 weeks after weaning, remaining lean. However feeding control (CD) DIO periconceptional/gestation/lactation period led pronounced sex-specific shift (17% 43%) from susceptibility resistance HFD,...
The ways in which epigenetic modifications fix the effects of early environmental events, ensuring sustained responses to transient stimuli, result modified gene expression patterns and phenotypes later life, is a topic considerable interest. This review focuses on recently discovered mechanisms calls into question prevailing views about dynamics, position functions marks. Most studies have addressed long-term small number marks, at global or individual level, stressors humans animal models....
According to the Developmental Origin of Health and Disease (DOHaD) concept, maternal obesity accelerated growth in neonates predispose offspring white adipose tissue (WAT) accumulation. In rodents, adipogenesis mainly develops during lactation. The mechanisms underlying phenomenon known as developmental programming remain elusive. We previously reported that adult rat from high-fat diet-fed dams (called HF) exhibited hypertrophic adipocyte, hyperleptinemia increased leptin mRNA levels a...
The phenotype of an individual is the result complex interactions between genome, epigenome and current, past ancestral environment leading to a lifelong remodelling epigenomes. genetic information expression contained in genome controlled by labile chromatin-associated epigenetic marks. Epigenetic misprogramming during development widely thought have persistent effect on health offspring may even be transmitted next generation. serves as interface genome. Dietary factors, including folate...
Abstract Highly differentiated mature spermatozoa carry not only genetic but also epigenetic information that is to be transmitted the embryo. DNA methylation one actor associated with sperm nucleus compaction, gene silencing, and prepatterning of embryonic expression. Therefore, stability this mark toward reproductive biotechnologies a major issue in animal production. The present work explored impact hormonal induction spermiation cryopreservation two cyprinids, goldfish ( Carassius...
Once daily milking (ODM) induces a reduction in milk production when compared to twice (TDM). Unilateral ODM of one udder half and TDM the other half, enables study underlying mechanisms independently inter-individual variability (same genetic background) environmental factors. Our results show that first-calf heifers three CpG, located 10 kb upstream from CSN1S1 gene were methylated 33, 34 28%, respectively, after but these levels higher ODM, 38, 38 33%, respectively. These methylation much...