A5029383464- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Biochemical and Structural Characterization
- Antimicrobial Peptides and Activities
- RNA Research and Splicing
- Protein Hydrolysis and Bioactive Peptides
- Endoplasmic Reticulum Stress and Disease
- Proteins in Food Systems
- Infant Nutrition and Health
- Venomous Animal Envenomation and Studies
- Cellular transport and secretion
- Digestive system and related health
- Animal Genetics and Reproduction
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Caveolin-1 and cellular processes
- Regulation of Appetite and Obesity
- Milk Quality and Mastitis in Dairy Cows
- Rabbits: Nutrition, Reproduction, Health
- Nanoparticle-Based Drug Delivery
- Pulmonary Hypertension Research and Treatments
- Streptococcal Infections and Treatments
- Probiotics and Fermented Foods
- Reproductive Biology and Fertility
Institut de génétique et de développement de Rennes
2016-2024
Université de Rennes
2015-2024
Centre National de la Recherche Scientifique
2015-2024
AgroParisTech
2015
Génétique Animale et Biologie Intégrative
2014-2015
Inserm
2015
Hypertension pulmonaire : physiopathologie et innovation thérapeutique
2015
Université Paris-Sud
2015
Assistance Publique – Hôpitaux de Paris
2015
Amsterdam UMC Location Vrije Universiteit Amsterdam
2015
Background— The vascular remodeling responsible for pulmonary arterial hypertension (PAH) involves predominantly the accumulation of α-smooth muscle actin–expressing mesenchymal-like cells in obstructive lesions. Endothelial-to-mesenchymal transition (EndoMT) may be a source those cells. Methods and Results— In situ evidence EndoMT human PAH was obtained by using confocal microscopy multiple fluorescent stainings at level, transmission electron correlative light ultrastructural level....
There is a great deal of interest in the development nanoplatforms gathering versatility and multifunctionality. The strategy reported herein meets these requirements further integrates cell-friendly shell bio-inspired approach. By taking advantage cell mechanism biomolecule transport using vesicles, we engineered hybrid biogenic nanoplatform able to encapsulate set nanoparticles regardless their chemistry or shape. As proof versatility, different types nanovesicles were produced: magnetic,...
Surface proteins of Gram-positive bacteria play crucial roles in bacterial adhesion to host tissues. Regarding commensal or probiotic bacteria, intestinal mucosa may promote their persistence the gastro-intestinal tract and beneficial effects host. In this study, seven Lactococcus lactis strains exhibiting variable surface physico-chemical properties were compared for Caco-2 epithelial cells. test, only one vegetal isolate TIL448 expressed a high-adhesion phenotype. A nonadhesive derivative...
Abstract In bacteria, trans -translation is the main rescue system, freeing ribosomes stalled on defective messenger RNAs. This mechanism driven by small protein B (SmpB) and transfer-messenger RNA (tmRNA), a hybrid known to have both tRNA-like an mRNA-like domain. Here we present four cryo-EM structures of ribosome during at resolutions from 3.0 3.4 Å. These include high-resolution structure whole pre-accommodated state, as well accommodated translocated translocation intermediate....
Summary Bacterial adhesion is a critical step for colonization of the host. The pioneer colonizer and commensal bacterium human gastrointestinal tract, Streptococcus salivarius , has strong adhesive properties but molecular determinants this remain uncharacterized. Serine‐rich repeat (SRR) glycoproteins are family adhesins that fulfil an important role in adhesion. In general, Gram‐positive bacterial genomes have unique SRR glycoprotein‐encoding gene. We demonstrate S. expresses three large...
Article12 March 2021Open Access Transparent process Reflux of Endoplasmic Reticulum proteins to the cytosol inactivates tumor suppressors Daria Sicari orcid.org/0000-0003-3847-129X Inserm U1242, University Rennes, France Centre de lutte contre le cancer Eugène Marquis, Search for more papers by this author Federica G Centonze Raphael Pineau Pierre-Jean Le Reste Neurosurgery Department, Hospital Luc Negroni orcid.org/0000-0002-6204-3917 Institut Génétique et Biologie Moléculaire Cellulaire,...
Abstract The multidomain ribosomal protein bS1 is the biggest and most flexible dynamic in 30S small subunit. Despite being essential for mRNA recruitment its primary role accommodation of start codon within decoding centre, there has not yet been a high-resolution description structure. Here, we present 3D atomic model OB1 OB2, bS1’s first two N-terminal domains, bound to an elongation-competent 70S ribosome. Our structure reveals that, as previously reported, anchored both by π-stacking...
Once daily milking (ODM) induces a reduction in milk production when compared to twice (TDM). Unilateral ODM of one udder half and TDM the other half, enables study underlying mechanisms independently inter-individual variability (same genetic background) environmental factors. Our results show that first-calf heifers three CpG, located 10 kb upstream from CSN1S1 gene were methylated 33, 34 28%, respectively, after but these levels higher ODM, 38, 38 33%, respectively. These methylation much...
During lactation, mammary epithelial cells secrete huge amounts of milk from their apical side. The current view is that caseins are secreted by exocytosis, whereas fat globules released budding, enwrapped the plasma membrane. Owing to number and large size globules, membrane surface needed for release might exceed A large-scale proteomics analysis both cytoplasmic lipid droplets globule membranes was used decipher cellular origins Surprisingly, differential protein profiles these two...
Abstract Toxins of toxin-antitoxin systems use diverse mechanisms to control bacterial growth. Here, we focus on the deleterious toxin atypical tripartite toxin-antitoxin-chaperone (TAC) system Mycobacterium tuberculosis , whose inhibition requires concerted action antitoxin and its dedicated SecB-like chaperone. We show that TAC is a bona fide ribonuclease identify exact cleavage sites in mRNA targets transcriptome-wide scale vivo. by occurs after second nucleotide ribosomal A-site codon...
Mechanisms underlying the transition from commensalism to virulence in Enterococcus faecalis are not fully understood. We previously identified enterococcal leucine-rich protein A (ElrA) as a factor of E. faecalis. The elrA gene is part an operon that comprises four other ORFs encoding putative surface proteins unknown function. In this work, we compared susceptibility phagocytosis three strains, including wild-type (WT), ΔelrA strain, and strain overexpressing whole elr order understand...
During translation's elongation cycle, factor G (EF-G) promotes messenger and transfer RNA translocation through the ribosome. Until now, structures reported for EF-G–ribosome complexes have been obtained by trapping EF-G in These results were based on use of non-hydrolyzable guanosine 5′-triphosphate (GTP) analogs, specific inhibitors or a mutated form. Here, we present first cryo-electron microscopy structure bound to ribosome absence an inhibitor. The reveals natural conformation EF-G·GDP...
Epithelial–mesenchymal transition (EMT) involves profound changes in cell morphology, driven by transcriptional and epigenetic reprogramming. However, evidence suggests that translation ribosome composition also play key roles establishing pathophysiological phenotypes. Using genome-wide analyses, we reported significant rearrangement of the translational landscape machinery during EMT. Specifically, a line overexpressing EMT transcription factor ZEB1 displayed alterations reprogramming...
Abstract Although conversion of the cellular form prion protein (PrP C ) into a misfolded isoform is underlying cause diseases, understanding PrP physiological functions has remained challenging. depletion or overexpression alters proliferation and differentiation properties various types stem progenitor cells in vitro by unknown mechanisms. Such involvement remains uncertain vivo absence any drastic phenotype mice lacking . Here, we report enrichment at base primary cilium from central...
West Nile Virus (WNV) is a zoonotic mosquito-transmitted flavivirus that can infect and cause disease in mammals including humans. Our study aimed at developing WNV vectored vaccine based on fish Novirhabdovirus, the Viral Hemorrhagic Septicemia virus (VHSV). VHSV replicates temperatures lower than 20°C naturally inactivated higher temperatures. A reverse genetics system has recently been developed our laboratory for allowing addition of genes viral genome recovery respective recombinant...
Alterations to the metabolic endocrine environment during early life are crucial mammary gland development. Among these environmental parameters, initial nutritional event after birth is consumption of milk, which represents first maternal support provided mammalian newborns. Milk a complex fluid that exerts effects far beyond its immediate value. The present study, therefore, aimed determine effect changes neonatal and prepubertal periods on adult phenotype. Newborn rabbits were suckled by...
Abstract Background The increase in bacterial resistance phenotype cases is a global health problem. New strategies must be explored by the scientific community order to create new treatment alternatives. Animal venoms are good source for antimicrobial peptides (AMPs), which excellent candidates drug development. Cathelicidin-related (CRAMPs) from snake have been studied as model design of pharmaceuticals against infections. Results In this study we present an 11 amino acid-long peptide,...