A5101666730- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- RNA Research and Splicing
- Vibrio bacteria research studies
- Enzyme Structure and Function
- Microbial Natural Products and Biosynthesis
- Antibiotic Resistance in Bacteria
- Legionella and Acanthamoeba research
- Protein purification and stability
- Chemical Synthesis and Analysis
- Microbial Metabolic Engineering and Bioproduction
- Bone fractures and treatments
- Endoplasmic Reticulum Stress and Disease
- Protein Kinase Regulation and GTPase Signaling
- Reproductive Biology and Fertility
- Lexicography and Language Studies
- Natural product bioactivities and synthesis
- DNA and Nucleic Acid Chemistry
- Protist diversity and phylogeny
- Diet and metabolism studies
- Cancer Treatment and Pharmacology
- Health, Medicine and Society
Birkbeck, University of London
2020-2024
Institut de génétique et de développement de Rennes
2015-2024
Centre National de la Recherche Scientifique
2016-2024
Institute of Structural and Molecular Biology
2020-2024
Université de Rennes
2018-2024
University College London
2020-2023
Bacterial conjugation is the fundamental process of unidirectional transfer DNAs, often plasmid from a donor cell to recipient cell1. It primary means by which antibiotic resistance genes spread among bacterial populations2,3. In Gram-negative bacteria, mediated large transport apparatus-the conjugative type IV secretion system (T4SS)-produced and embedded in both its outer inner membranes. The T4SS also elaborates long extracellular filament-the pilus-that essential for DNA transfer4,5....
Abstract Legionella pneumophila is a bacterial pathogen that utilises Type IV secretion (T4S) system to inject effector proteins into human macrophages. Essential the recruitment and delivery of effectors T4S machinery membrane-embedded T4 coupling complex (T4CC). Here, we purify an intact T4CC from membrane. It contains DotL ATPase, DotM DotN proteins, chaperone module IcmSW, two previously uncharacterised DotY DotZ. The atomic resolution structure reveals DotLMNYZ hetero-pentameric core...
Abstract Conjugative type IV secretion systems (T4SS) mediate bacterial conjugation, a process that enables the unidirectional exchange of genetic materials between donor and recipient cell. Bacterial conjugation is primary means by which antibiotic resistance genes spread among populations (Barlow 2009; Virolle et al, 2020). T4SSs form pili: long extracellular filaments connect with cells. Previously, we solved cryo-electron microscopy (cryo-EM) structure conjugative T4SS. In this article,...
Protein structure and protein-protein interaction (PPI) predictions based on coevolution have transformed structural biology, but managing pre-processing post-processing can be complex time-consuming, making these tools less accessible. Here, we introduce PPIFold, a pipeline built the AlphaPulldown Python package, designed to automate file handling streamline generation of outputs, facilitating interpretation PPI prediction results. The was validated bacterial Type 4 Secretion System...
BackgroundPLCγ enzymes are key nodes in cellular signal transduction and their mutated rare variants have been recently implicated development of a range diseases with unmet need including cancer, complex immune disorders, inflammation neurodegenerative diseases. However, molecular nature activation the impact dysregulation mechanisms by mutations, remain unclear; both critically dependent on comprehensive characterization intact PLCγ enzymes.MethodsFor structural studies we applied cryo-EM,...
During translation's elongation cycle, factor G (EF-G) promotes messenger and transfer RNA translocation through the ribosome. Until now, structures reported for EF-G–ribosome complexes have been obtained by trapping EF-G in These results were based on use of non-hydrolyzable guanosine 5′-triphosphate (GTP) analogs, specific inhibitors or a mutated form. Here, we present first cryo-electron microscopy structure bound to ribosome absence an inhibitor. The reveals natural conformation EF-G·GDP...
Legionella pneumophila is an opportunistic pathogen infecting alveolar macrophages and protozoa species. utilizes a Type IV Secretion System (T4SS) to translocate over 300 effector proteins into its host cell. In recent study, we have isolated solved the cryo-EM structure of Coupling Complex (T4CC), large cytoplasmic determinant associated with inner membrane that recruits for delivery T4SS translocation. The T4CC composed DotLMNYZ hetero-pentameric core from which flexible IcmSW module...
ABSTRACT Conjugative Type IV Secretion Systems (T4SS) mediate bacterial conjugation, a process that enables the unidirectional exchange of genetic materials between donor and recipient cell. Bacterial conjugation is primary means by which antibiotic resistance genes spread among populations (Barlow, 2009; Virolle et al , 2020). T4SSs elaborate long extracellular filament, termed “pilus” to connect with Previously, we solved cryo-EM structure conjugative T4SS. In this article, based on...