A5055534269- Microtubule and mitosis dynamics
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Protist diversity and phylogeny
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- Photosynthetic Processes and Mechanisms
- 14-3-3 protein interactions
- Advanced Electron Microscopy Techniques and Applications
- Cellular transport and secretion
- Microbial Community Ecology and Physiology
- Advanced Thermodynamics and Statistical Mechanics
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Methane Hydrates and Related Phenomena
- Cellular Mechanics and Interactions
Institut de génétique et de développement de Rennes
2017-2023
Université de Rennes
2019-2023
Centre National de la Recherche Scientifique
2016-2023
Aix-Marseille Université
2016
Abstract Viruses modulate ecosystems by directly altering host metabolisms through auxiliary metabolic genes. However, viral genomes are not known to encode the core components of translation machinery, such as ribosomal proteins (RPs). Here, using reference and global-scale metagenomic datasets, we identify 14 different RPs across arising from cultivated isolates metagenome-assembled viruses. tend dynamic RPs, easily exchangeable between ribosomes, suggesting these can replace cellular...
Abstract In bacteria, trans -translation is the main rescue system, freeing ribosomes stalled on defective messenger RNAs. This mechanism driven by small protein B (SmpB) and transfer-messenger RNA (tmRNA), a hybrid known to have both tRNA-like an mRNA-like domain. Here we present four cryo-EM structures of ribosome during at resolutions from 3.0 3.4 Å. These include high-resolution structure whole pre-accommodated state, as well accommodated translocated translocation intermediate....
Microtubules are tubes of about 25 nm in diameter that critically involved a variety cellular functions, including motility, compartmentalization, and division. They considered as pseudo-helical polymers whose constituent αβ-tubulin heterodimers share lateral homotypic interactions, except at one unique region called the seam. Here, we used segmented sub-tomogram averaging strategy to reassess this paradigm analyze organization microtubules assembled from purified porcine brain tubulin...
Abstract In order to discover new antibiotics with improved activity and selectivity, we created a reliable in vitro reporter system detect trans-translation activity, the main mechanism for recycling ribosomes stalled on problematic messenger RNA (mRNA) bacteria. This is based an engineered tmRNA variant that reassembles green fluorescent protein (GFP) when active. Our adapted high-throughput screening of chemical compounds by fluorescence.
The arrest of protein synthesis caused when ribosomes stall on an mRNA lacking a stop codon is deadly risk for all cells. In bacteria, this situation remedied by the trans‐ translation quality control system. Trans ‐translation occurs because synergistic action two main partners, transfer‐messenger RNA (tmRNA) and small B (SmpB). These act in complex to monitor synthesis, intervening necessary rescue stalled ribosomes. During process, incomplete nascent peptides are tagged destruction,...
Abstract Microtubules are tubes of about 25 nm in diameter that critically involved a variety cellular functions including motility, compartmentalization, and division. They considered as pseudo-helical polymers whose constituent αβ-tubulin heterodimers share lateral homotypic interactions, except at one unique region called the seam. Here, we used segmented sub-tomogram averaging strategy to reassess this paradigm analyze organization microtubules assembled from purified porcine brain...
The α-β tubulin heterodimer undergoes subtle conformational changes during microtubule assembly. These can be modulated by external factors, whose effects on structure characterized 2D views obtained cryo-electron microscopy. Analysis of images is facilitated if they are straight enough to interpret and filter their image Fourier transform, which provide useful information concerning the arrangement molecules inside lattice. Here, we describe use TubuleJ software straighten microtubules...
Viruses modulate ecosystems by directly altering host metabolisms through auxiliary metabolic genes, which are obtained random sampling of the genome and rise to fixation, presumably improved viral fitness alleviating key bottlenecks during infection. Conspicuously, however, genomes not known encode core components translation machinery, such as ribosomal proteins (RPs), though genes for RPs S1 S21 have been detected in metagenomes. Here we augment this little-noticed observation using...
At first glance, the structure of a microtubule is simple. Globular α- and β-tubulin subunits form constitutive heterodimers that align head-to-tail in protofilaments. In most common configuration, 13 protofilaments associate laterally with slight longitudinal stagger results left-handed 3-start helix featuring lateral associations between tubulin subunits. This seemingly straightforward description actually based on almost half century research aimed at understanding how dimers interact...
Key features• This protocol builds upon the method developed by J.M. Heumann to perform subtomogram averages of microtubules and extends it divide them into shorter segments.• Microtubules are decorated with kinesin motor-domains determine underlying organization its constituent αβ-tubulin heterodimers.• The SSTA approach allows analysis structural heterogeneity individual reveals multiseams changes in their number location within shaft.