A5030608960- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Metabolism and Genetic Disorders
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and Molecular Research
- Protein Structure and Dynamics
- Pharmacological Effects of Natural Compounds
- Endoplasmic Reticulum Stress and Disease
- Antibiotic Resistance in Bacteria
- Viral Infections and Immunology Research
- RNA Research and Splicing
Institut de génétique et de développement de Rennes
2021-2023
Université de Rennes
2021-2023
Centre National de la Recherche Scientifique
2021-2023
University of Salerno
2021
Abstract In bacteria, trans -translation is the main rescue system, freeing ribosomes stalled on defective messenger RNAs. This mechanism driven by small protein B (SmpB) and transfer-messenger RNA (tmRNA), a hybrid known to have both tRNA-like an mRNA-like domain. Here we present four cryo-EM structures of ribosome during at resolutions from 3.0 3.4 Å. These include high-resolution structure whole pre-accommodated state, as well accommodated translocated translocation intermediate....
Abstract The multidomain ribosomal protein bS1 is the biggest and most flexible dynamic in 30S small subunit. Despite being essential for mRNA recruitment its primary role accommodation of start codon within decoding centre, there has not yet been a high-resolution description structure. Here, we present 3D atomic model OB1 OB2, bS1’s first two N-terminal domains, bound to an elongation-competent 70S ribosome. Our structure reveals that, as previously reported, anchored both by π-stacking...
Abstract Toxins of toxin-antitoxin systems use diverse mechanisms to control bacterial growth. Here, we focus on the deleterious toxin atypical tripartite toxin-antitoxin-chaperone (TAC) system Mycobacterium tuberculosis , whose inhibition requires concerted action antitoxin and its dedicated SecB-like chaperone. We show that TAC is a bona fide ribonuclease identify exact cleavage sites in mRNA targets transcriptome-wide scale vivo. by occurs after second nucleotide ribosomal A-site codon...
The third step of the catabolism galactose in mammals is catalyzed by enzyme galactose-1-phosphate uridylyltransferase (GALT), a homodimeric with two active sites located proximity intersubunit interface. Mutations this are associated to rare inborn error metabolism known as classic galactosemia; particular, most common mutation, severe phenotype, one that replaces Gln188 site Arg (p.Gln188Arg). In past, and more recently, structural effects mutation were deduced on static structure...
The arrest of protein synthesis caused when ribosomes stall on an mRNA lacking a stop codon is deadly risk for all cells. In bacteria, this situation remedied by the trans‐ translation quality control system. Trans ‐translation occurs because synergistic action two main partners, transfer‐messenger RNA (tmRNA) and small B (SmpB). These act in complex to monitor synthesis, intervening necessary rescue stalled ribosomes. During process, incomplete nascent peptides are tagged destruction,...
Classic galactosemia is an inborn error of metabolism associated with mutations that impair the activity and stability galactose-1-phosphate uridylyltransferase (GALT), catalyzing third step in galactose metabolism. To date, no treatments (including dietary deprivation) are able to prevent or alleviate long-term complications affecting galactosemic patients. Evidence arginine improve human enzyme expressed a prokaryotic model classic has induced researchers suppose this amino acid could act...