A5021484885- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
- Ferroptosis and cancer prognosis
- RNA regulation and disease
- Cancer Cells and Metastasis
- Adenosine and Purinergic Signaling
- Advanced Proteomics Techniques and Applications
- Single-cell and spatial transcriptomics
- Cancer-related gene regulation
- Pancreatic function and diabetes
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Kruppel-like factors research
- Extracellular vesicles in disease
- Cellular transport and secretion
- Genetics, Bioinformatics, and Biomedical Research
- Genomics and Phylogenetic Studies
- Glioma Diagnosis and Treatment
- Photosynthetic Processes and Mechanisms
- Autophagy in Disease and Therapy
- Vibrio bacteria research studies
- Renal cell carcinoma treatment
- RNA and protein synthesis mechanisms
- Genetic and Kidney Cyst Diseases
- Biotin and Related Studies
Edinburgh Cancer Research
2015-2025
University Kebangsaan Malaysia Medical Centre
2018-2024
National University of Malaysia
2018-2024
Universiti Putra Malaysia
2024
University of Edinburgh
2018-2023
Institute of Genetics and Cancer
2018
National Institutes of Biotechnology Malaysia
2018
University of Pennsylvania
2013
Glioblastoma multiforme (GBM) is a highly lethal, stage IV brain tumour with prevalence of approximately 2 per 10,000 people globally. The cell surface proteins or surfaceome serve as information gateway in many oncogenic signalling pathways and are important modulating cancer phenotypes. Dysregulation expression activity have been shown to promote tumorigenesis. GBM case point; OMICS screening cell-based system identified that this sub-proteome largely perturbed GBM. Additionally, since...
Article12 March 2021Open Access Transparent process Reflux of Endoplasmic Reticulum proteins to the cytosol inactivates tumor suppressors Daria Sicari orcid.org/0000-0003-3847-129X Inserm U1242, University Rennes, France Centre de lutte contre le cancer Eugène Marquis, Search for more papers by this author Federica G Centonze Raphael Pineau Pierre-Jean Le Reste Neurosurgery Department, Hospital Luc Negroni orcid.org/0000-0002-6204-3917 Institut Génétique et Biologie Moléculaire Cellulaire,...
Clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing is evolving into an essential tool in the field of biological and medical research. Notably, development catalytically deactivated Cas9 (dCas9) enzyme has substantially broadened its traditional boundaries gene or perturbation. The conjugation dCas9 with various molecular effectors allows precise control over transcriptional processes, epigenetic modifications, visualization chromosomal dynamics, several...
Breast cancer is the leading cause of cancer-related deaths in women. The aggressive breast subtype commonly linked to genetic alterations TP53 tumor suppressor gene, predominantly missense mutations. Robust experimental models are needed gain better insights into these mutations’ molecular properties and implications tumorigenesis. generation such harboring feasible with CRISPR-based gene editing technology. Moreover, development new CRISPR applications, particularly DNA base prime editing,...
AGR2 is an oncogenic endoplasmic reticulum (ER)-resident protein disulfide isomerase. has a relatively unique property for chaperone in that it can bind sequence-specifically to specific peptide motif (TTIYY). A synthetic TTIYY-containing column was used affinity-purify from crude lysates highlighting selectivity complex mixtures. Hydrogen-deuterium exchange mass spectrometry localized the dominant region interacts with TTIYY within structural loop amino acids 131–135 (VDPSL). binding site...
The detection of cancer antigens is a major aim research in order to develop better patient management through early disease detection. Many cancers including prostate, lung, and ovarian secrete protein disulfide isomerase named AGR2 that has been previously detected urine plasma using mass spectrometry. Here we determine whether developed monoclonal antibody targeting can be adapted from an indirect two-site ELISA format into direct detector solid-phase printed gold electrodes....
Cytochrome c maturation (Ccm) is a post-translational process that occurs after translocation of apocytochromes to the positive (p) side energy-transducing membranes. Ccm responsible for formation covalent bonds between thiol groups two cysteines residues at heme-binding sites and vinyl heme b (protoporphyrin IX-Fe). Among proteins (CcmABCDEFGHI CcdA) required this process, CcmABCD are involved in loading apoCcmE. The holoCcmE thus formed provides apocytochromes. Catalysis thioether mediated...
Clear cell renal carcinoma (ccRCC) is a hypervascular tumor that characterized by bi-allelic inactivation of the VHL suppressor gene and mTOR signalling pathway hyperactivation. The pro-angiogenic factor PDGFB, transcriptional target super enhancer-driven KLF6, can activate mTORC1 in ccRCC. However, detailed mechanisms PDGFB-mediated activation ccRCC have remained elusive. Here, we investigated whether cells are able to secrete PDGFB into extracellular milieu stimulate activity. We found...