A5079658433- RNA modifications and cancer
- Antimicrobial Resistance in Staphylococcus
- SARS-CoV-2 detection and testing
- Bacterial biofilms and quorum sensing
- Biosensors and Analytical Detection
- Bacterial Identification and Susceptibility Testing
- Genetics and Neurodevelopmental Disorders
- Advanced biosensing and bioanalysis techniques
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Melanoma and MAPK Pathways
- Bacterial Genetics and Biotechnology
- Cancer-related molecular mechanisms research
- Cellular Mechanics and Interactions
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Microtubule and mitosis dynamics
- Lung Cancer Treatments and Mutations
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Hippo pathway signaling and YAP/TAZ
- Lung Cancer Diagnosis and Treatment
- COVID-19 Impact on Reproduction
- Congenital heart defects research
Université Côte d'Azur
2020-2025
Centre Hospitalier Universitaire de Nice
2025
Inserm
2025
Hôpital Pasteur
2025
Centre National de la Recherche Scientifique
2020-2024
Institut de Pharmacologie Moléculaire et Cellulaire
2020-2024
Microbiologie de l’alimentation au service de la santé
2015-2022
AgroParisTech
2015-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022
Université Paris-Saclay
2022
Wastewater surveillance was proposed as an epidemiological tool to define the prevalence and evolution of SARS-CoV-2 epidemics. However, most implemented wastewater projects were based on qPCR measurement virus titers did not address mutational spectrum circulating in population.We have a nanopore RNA sequencing monitoring system city Nice (France, 550,000 inhabitants). Between October 2020 March 2021, we monthly analyzed variants 113 samples collected main treatment plant 20...
Hypoxic reprogramming of vasculature relies on genetic, epigenetic, and metabolic circuitry, but the control points are unknown. In pulmonary arterial hypertension (PAH), a disease driven by hypoxia inducible factor (HIF)–dependent vascular dysfunction, HIF-2α promoted expression neighboring genes, long noncoding RNA (lncRNA) histone lysine N -methyltransferase 2E-antisense 1 ( KMT2E-AS1 ) N-methyltransferase 2E KMT2E ). stabilized protein to increase epigenetic 3 4 trimethylation (H3K4me3),...
The brain cytoplasmic RNA, BC1, is a small non-coding RNA that found in different RNP particles, some of which are involved translational control. One component BC1-containing complexes the fragile X mental retardation protein (FMRP) implicated repression. Peptide mapping and computational simulations show tudor domain FMRP makes specific contacts to BC1 RNA. Endogenous 2′-O-methylated nucleotides contact interface, methylation can affect this interaction. In cell body 2′-O-methylations...
The fragile X syndrome (FXS), the most common form of inherited intellectual disability, is due to absence FMRP, a protein regulating RNA metabolism. Recently, an unexpected function FMRP in modulating activity Adenosine Deaminase Acting on (ADAR) enzymes has been reported both Drosophila and Zebrafish. ADARs are RNA-binding proteins that increase transcriptional complexity through post-transcriptional mechanism called editing. To evaluate ADAR2-FMRP interaction mammals we analyzed several...
Enterococcus faecalis is the third cause of nosocomial infections. To obtain first comprehensive view transcriptional organizations in this bacterium, we used a modified RNA-seq approach enabling to discriminate primary from processed 5'RNA ends. We also validated our by confirming known features Escherichia coli. mapped 559 transcription start sites and 352 processing E. faecalis. A blind motif search retrieved canonical SigA- SigN-dependent promoters preceding TSSs mapped. discovered 95...
Article14 February 2022Open Access Source DataTransparent process Blockade of the pro-fibrotic reaction mediated by miR-143/-145 cluster enhances responses to targeted therapy in melanoma Serena Diazzi Université Côte d'Azur, INSERM, C3M, Nice, France CNRS, Institut de Pharmacologie Moléculaire et Cellulaire (IPMC), Sophia Antipolis, Equipe labellisée Ligue Contre le Cancer, Contribution: Conceptualization, Formal analysis, Investigation, Methodology, Writing - original draft, review &...
Enterococcus faecalis is a natural inhabitant of the human gastrointestinal tract. This bacterial species subdominant in healthy physiological state gut microbiota (eubiosis) adults, but can become dominant and cause infections when intestinal homeostasis disrupted (dysbiosis). The relatively high concentrations bile acids deoxycholate (DCA) taurocholate (TCA) hallmark eubiosis dysbiosis, respectively. study aimed to better understand how E. adapts DCA TCA. We showed that impairs growth...
Background: In 2020, people living with HIV (PLHIV) in France were worried that the COVID-19 health crisis would lead to long-term changes their care. Using data from anonymous, online, cross-sectional survey ACOVIH, which was completed by PLHIV between July and September this study explored factors associated worry about care after end of first lockdown (17 March-11 May 2020). Methods: multivariate logistic regression, we compared participants who declared they those did not, terms...
Dopamine D 2 receptor (D DR)-mediated transmission in the striatum is remarkably flexible, and changes its efficacy have been heavily implicated a variety of physiological pathological conditions. Although receptor-associated proteins are clearly involved specific forms synaptic plasticity, molecular mechanisms regulating sensitivity receptors this brain area essentially obscure. We studied responses DR stimulations mice lacking cytoplasmic RNA BC1, small noncoding dendritically localized...
The emergence and quick spread of SARS-CoV-2 has pointed at a low capacity response for testing large populations in many countries, line material, technical staff limitations. traditional RT-qPCR diagnostic test remains the reference method is by far most widely used test. These assays are limited to few probe sets, require sample PCR reaction volumes, along with an expensive time-consuming RNA extraction step. Here we describe quantitative nanofluidic assay that overcomes some these...
We discovered a chromosomal locus containing 2 toxin-antitoxin modules (TAs) with an antisense transcriptional organization in the E. faecalis clinical isolate V583. These TAs are homologous to type I txpA-ratA system and II mazEF, respectively. have shown that putative MazF is toxic for coli triggers RNA degradation, its cognate antitoxin MazE counteracts toxicity. The second module, adjacent expresses toxin predicted belong TxpA family found Firmicutes, antidote, RatA. Genomic analysis...
Mutations or deletions of FMRP, involved in the regulation mRNA metabolism brain, lead to Fragile X syndrome (FXS), most frequent form inherited intellectual disability. A severe manifestation disease has been associated with Ile304Asn mutation, located on KH2 domain protein. Several hypotheses have proposed explain possible molecular mechanism responsible for drastic effect this mutation humans. Here, we performed a dynamics simulation and show that destabilizes hydrophobic core producing...
The Mtq2-Trm112 methyltransferase modifies the eukaryotic translation termination factor eRF1 on glutamine side chain of a universally conserved GGQ motif that is essential for release newly synthesized peptides. Although this modification found in three domains life, its exact role eukaryotes remains unknown. As deletion MTQ2 leads to severe growth impairment yeast, we have investigated further and tested putative involvement ribosome biogenesis. We Mtq2 associated with nuclear 60S subunit...
Mechanisms underlying the transition from commensalism to virulence in Enterococcus faecalis are not fully understood. We previously identified enterococcal leucine-rich protein A (ElrA) as a factor of E. faecalis. The elrA gene is part an operon that comprises four other ORFs encoding putative surface proteins unknown function. In this work, we compared susceptibility phagocytosis three strains, including wild-type (WT), ΔelrA strain, and strain overexpressing whole elr order understand...
Single-cell CRISPR-based transcriptome screens are potent genetic tools for concomitantly assessing the expression profiles of cells targeted by a set guides RNA (gRNA), and inferring target gene functions from observed perturbations. However, due to various limitations, this approach lacks sensitivity in detecting weak perturbations is essentially reliable when studying master regulators such as transcription factors. To overcome challenge subtle gRNA induced transcriptomic classifying most...
Transcription initiation and RNA processing govern gene expression enable bacterial adaptation by reshaping the landscape. The aim of this study was to simultaneously observe these two fundamental processes in a transcriptome responding an environmental signal. A controlled σ E system E. coli coupled our previously described tagRNA-seq method yield process kinetics information. Changes transcription frequencies (TIF) (PF) were followed using 5′ tags. TIF showed binary increased/decreased...
Abstract Background Wastewater surveillance has been proposed as an epidemiological tool to define the prevalence and evolution of SARS-CoV-2 epidemics. However, most implemented wastewater projects were relying on qPCR measurement virus titers did not address mutational spectrum circulating in population. Methods We have a nanopore RNA sequencing monitoring system city Nice (France, 550,000 inhabitants). Between October 2020 March 2021, we monthly analyzed variants 113 samples collected...
e20018 Background: Liquid biopsy (LB) is a useful tool for the detection of actionable mutations in advanced non-squamous non-small cell lung cancer (NS-NSCLC) at progression. However, clinical benefits integrating both LB and tissue (TB)-based genomic profiling diagnosis NS-NSCLC remain uncertain. Methods: We tested three commercially available next-generation sequencing (NGS) gene panel assays circulating cell-free DNA (cfDNA) using replicate sets 85 samples from patients baseline compared...
COVID-19 vaccination is crucial to reduce the incidence of severe forms disease in population. However, people who use drugs (PWUD) face structural and individual barriers vaccination, little known about intention factors associated with that among PWUD. This study aimed estimate PWUD early stage campaigns.
Abstract The emergence and quick spread of SARS-CoV-2 has pointed at a low capacity response for testing large populations in many countries, line material, technical staff limitations. traditional RT-qPCR diagnostic test remains the reference method is by far most widely used test. These assays are limited to couple probe sets, require sample PCR reaction volumes, along with an expensive time-consuming RNA extraction steps. Here we describe quantitative nanofluidic assay that overcomes some...
Overactivation of the mitogen-activated protein kinase (MAPK) pathway is a critical driver many human cancers. However, therapies directly targeting this lead to cancer drug resistance. Resistance has been linked compensatory RAS overexpression, but mechanisms underlying response remain unclear. Here, we find that MEK inhibitors (MEKi) are associated with an increased translation KRAS and NRAS oncogenes through mechanism involving dissolution processing body (P-body) biocondensates. This...
Abstract Single-cell CRISPR-based transcriptome screens are potent genetic tools for concomitantly assessing the expression profiles of cells targeted by a set guides RNA (gRNA), and inferring target gene functions from observed perturbations. However, due to various limitations, this approach lacks sensitivity in detecting weak perturbations is essentially reliable when studying master regulators such as transcription factors. To overcome challenge subtle gRNA induced transcriptomic...
Abstract Mechanical signals regulate cell shape and influence metabolism behavior. Cells withstand external forces by adjusting the stiffness of its cytoskeleton. Microtubules (MTs) act as compression-bearing elements in response to mechanical cues. Therefore, MT dynamics affect mechanics. Yet, how loads control adjust mechanics locally constrained environment has remained unclear. Here, we show that rewire glutamine promote glutamylation force mechanics, thereby modulating mechanodependent...