A5008299187- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Genetics and Reproduction
- Reproductive biology and impacts on aquatic species
- Fish Ecology and Management Studies
- Wildlife Ecology and Conservation
- Aquaculture Nutrition and Growth
- Fish Biology and Ecology Studies
- Genetic and phenotypic traits in livestock
- MicroRNA in disease regulation
- Acute Myeloid Leukemia Research
- Reproductive Biology and Fertility
- Chromosomal and Genetic Variations
- Animal Behavior and Reproduction
- Sexual Differentiation and Disorders
- Ecology and biodiversity studies
- Wnt/β-catenin signaling in development and cancer
- Study of Mite Species
- Aquaculture disease management and microbiota
- Protist diversity and phylogeny
- CRISPR and Genetic Engineering
- Plant Physiology and Cultivation Studies
- Histone Deacetylase Inhibitors Research
- Eicosanoids and Hypertension Pharmacology
- Fungal Biology and Applications
- Gender Studies in Language
University of Tsukuba
2024
Fish Physiology and Genomics Institute
2012-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2022
Niigata University of Health and Welfare
2021
University of Shiga Prefecture
2017
Kyoto University
2016
Genopole (France)
2013-2014
Cancéropôle Grand Ouest
2014
Université de Rennes
2014
Institut National de la Recherche Agronomique
2011-2012
Abstract All salmonid species investigated to date have been characterized with a male heterogametic sex‐determination system. However, as these do not share any Y‐chromosome conserved synteny, there remains debate on whether they common master sex‐determining gene. In this study, we the extent of conservation and evolution rainbow trout ( O ncorhynchus mykiss ) gene, sdY sexually dimorphic ), in 15 different salmonids. We found that sequence is highly all salmonids male‐specific gene...
Teleost fishes, thanks to their rapid evolution of sex determination mechanisms, provide remarkable opportunities study the formation chromosomes and mechanisms driving birth new master determining (MSD) genes. However, evolutionary interplay between MSD genes they harbor is rather unexplored. We characterized a male-specific duplicate anti-Müllerian hormone (amh) as gene in Northern Pike (Esox lucius), using genomic expression evidence well by loss-of-function gain-of-function experiments....
Runt-related transcription factor 1 (RUNX1) is generally considered to function as a tumor suppressor in the development of leukemia, but growing body evidence suggests that it has pro-oncogenic properties acute myeloid leukemia (AML). Here we have demonstrated antileukemic effect mediated by RUNX1 depletion highly dependent on functional p53-mediated cell death pathway. Increased expression other RUNX family members, including RUNX2 and RUNX3, compensated for antitumor elicited silencing,...
Evolutionary novelties require rewiring of transcriptional networks and/or the evolution new gene functions. Sex determination (SD), one most plastic evolutionary processes, requires such novelties. Studies on vertebrate SD revealed that master genes are generally recruited from involved in downstream regulatory genetic network. Only a single exception to this rule is currently known vertebrates: intriguing case salmonid (sdY), which arose duplication an immune-related gene. This immediately...
Continuous or cyclic production of spermatozoa throughout life in adult male vertebrates depends on a subpopulation undifferentiated germ cells acting as spermatogonial stem (SSCs). What makes these self-renew differentiate is barely understood, particular nonmammalian species, including fish. In the highly seasonal rainbow trout, at end annual spermatogenetic cycle, tubules spawning testis contain only spermatozoa, with exception scarce spermatogonia that remain tubular wall and will...
There is a need to isolate different populations of spermatogenic cells investigate the molecular events that occur during spermatogenesis. Here we developed new method identify and purify testicular germ from rainbow trout (Oncorhynchus mykiss) carrying green fluorescent protein gene driven by vasa regulatory regions (pvasa-GFP) at various stages Rainbow piwi-like (rtili), scp3 (rt-scp3), shippo1 (rt-shippo1) were identified as markers for spermatogonia, spermatocytes, spermatids,...
Abstract The spermatogonia of fish can be classified as being either undifferentiated type A or differentiated B spermatogonia. Although spermatogonia, which contain spermatogonial stem cells, have been demonstrated to a suitable material for germ cell transplantation, no molecular markers distinguishing between and in developed date. We therefore sought develop marker rainbow trout. Using GFP‐dependent flow cytometry (FCM), enriched fractions testicular somatic primordial cells were...
Tbx1 is a member of the T-box transcription factor gene family involved in embryogenesis and organogenesis. Recently, within pan-genomic screen using rainbow trout (Oncorhynchus mykiss) cDNA microarrays, we identified tbx1 homolog with testicular over-expression during sex differentiation. Here, characterized two very similar paralogs, tbx1a tbx1b. In adult tissues, expression restricted to gonads, high testis, while tbx1b more widely expressed gills, brains, muscle, skin. During gonadal...
In mammals, follistatin (FST) plays an important role in early ovarian differentiation, acting downstream of the Wnt pathway. teleost fish, <i>fst</i> is implicated folliculogenesis and oocyte maturation, specific expression during differentiation has been described rainbow trout, <i>Oncorhynchus mykiss. </i>By situ hybridization, we demonstrated that trout gonadal shares a similar pattern with <i>cyp19a1a</i>, gene encoding aromatase, key steroidogenic...
Sex hormone-binding globulin (SHBG) binds androgens and estrogens in the blood of many vertebrates, including teleost fish. In mammals, SHBG is synthetized liver secreted into blood. fish, shbga also exhibits a hepatic expression. salmonids, which gene has been duplicated, recently discovered shbgb predominantly ovarian The present work aimed at gaining new insight structure expression during gonadal sex differentiation, steroid-sensitive process, Shbgb protein binding characteristics;...
Abstract Teleost fishes, thanks to their rapid evolution of sex determination mechanisms, provide remarkable opportunities study the formation chromosomes and mechanisms driving birth new master determining (MSD) genes. However, evolutionary interplay between MSD genes they harbor is rather unexplored. We characterized a male-specific duplicate anti-Müllerian hormone ( amh) as gene in Northern Pike Esox lucius ), using genomic expression evidences well by loss-of-function gain-of-function...
Throughout an organism's early development, variations in physiology and behaviours may have long lasting consequences on individual life histories. While a large part of variation critical life-history transitions remains unexplained, significant proportion be caused by gender effects as gender-specific histories shaped sexual selection. In this study, we investigated the presence timing emergence from gravel energetic status brown trout ( Salmo trutta ) stages. To investigate question,...
Abstract Many salmonids have a male heterogametic (XX/XY) sex determination system, and they are supposed to conserved master sex-determining gene (sdY) that interacts at the protein level with Foxl2 leading blockage of synergistic induction Nr5a1 cyp19a1a promoter. However, this hypothesis role sdY in is challenged by few exceptions, one them being presence naturally occurring “apparent” XY Chinook salmon, Oncorhynchus tshawytscha, females. Here, we show some salmon females (sdY-N183), 1...
Follistatin (Fst) is an inhibitor of some members the TGFβ family that was first identified in mammalian ovary. Fst gene has been shown to be expressed granulosa cells and oocyte fish species, involved folliculogenesis development. In addition these functions ovarian development, already mouse embryonic ovary, its inactivation induces partial female-to-male sex reversal, suggesting plays a significant role early differentiation mammals. However, only little known about potential gonadal...
The purpose of this study was to investigate the availability information on dietary supplements as well their use among high school students in Niigata Prefecture, Japan. It also aimed clarify relationship between awareness and supplements. survey conducted online using Google Forms asked about sex, age, height, weight, experience with (current use, past or never used any use), types used, sources supplements, eating habits. schools Japan, September 2020. approved by ethics committee...
ABSTRACT Many salmonids have a male heterogametic (XX/XY) sex determination system, and they are supposed to conserved master determining gene ( sdY ), that interacts at the protein level with Foxl2 leading blockage of synergistic induction Nr5a1 cyp19a1a promoter. However, this hypothesis role in is still challenged by few exceptions, one them being presence some naturally occurring “apparent” XY Chinook salmon females. Here we show females sdY-N183 which has missense mutation substitution...