Understanding the mechanisms that regulate germ-cell development is crucial to reproductive medicine and animal production. Animal gametes originally derive from sexually undifferentiated primordial germ cells (PGCs), which develop into mitotic (oogonia or spermatogonia) before proceeding meiosis [Wylie, C. (1999) Cell 96, 165–174]. Spermatogonia are thought include a population of with stem cell activity, proliferate throughout lifespan male animals produce spermatozoa [Zhao, G. Q. &...

Site-selective and direct C–F bond transformation of perfluoroalkylarenes was achieved with allylic stannanes via an iridium photoredox catalyst system. The present defluoroallylation proceeds exclusively at the benzylic position through perfluoroalkyl radicals generated by a single-electron transfer from excited to perfluoroalkylarenes. A variety groups are applicable: linear perfluoroalkyl-substituted arenes such as Ar–nC4F9 Ar–nC6F13 heptafluoroisopropylarenes (Ar–CF(CF3)2) underwent...

We report the visible-light-induced radical coupling reaction of silyl enol ethers with α-bromocarbonyl compounds to give 1,4-dicarbonyls. The was effectively accelerated using an inexpensive organic dye (eosin Y) as a photoredox catalyst. 1,4-Dicarbonyl alone were afforded, without generation carbonyl adducts α-halocarbonyls, which are usually generated in presence fluoride anions or Lewis acids. A variety ethers, α-bromoketones, α-bromoesters, and α-bromoamides applied this system produce...

A 57 kDa protein (p57) was obtained during the study on phosphatidylinositol‐specific phospholipase C. Its cDNA isolated from calf spleen and human leukemia cell line HL60 libraries cloned. In primary structures of p57, they have two unique amino acid sequence motifs, a WD repeat leucine zipper motif. Furthermore, p57 shared similarity (40%) with coronin, an actin‐binding responsible for chemotaxis, motility, cytokinesis Dictyostelium discoideum , which has only repeat. also showed activity...

There is a need to isolate different populations of spermatogenic cells investigate the molecular events that occur during spermatogenesis. Here we developed new method identify and purify testicular germ from rainbow trout (Oncorhynchus mykiss) carrying green fluorescent protein gene driven by vasa regulatory regions (pvasa-GFP) at various stages Rainbow piwi-like (rtili), scp3 (rt-scp3), shippo1 (rt-shippo1) were identified as markers for spermatogonia, spermatocytes, spermatids,...

Although in vitro replication of the hepatitis C virus (HCV) JFH1 clone genotype 2a (HCVcc) has been developed, a robust cell culture system for 1a and 1b genotypes, which are most prevalent viruses world resistant to interferon therapy, not yet established. As surrogate system, pseudotype transiently bearing HCV envelope proteins based on vesicular stomatitis (VSV) retrovirus have developed. Here, we developed replication-competent recombinant VSV with genome encoding unmodified E1 E2 place...

Abstract We have achieved the first anti ‐borylstannylation of alkynes by using ( o ‐phenylenediaminato)borylstannanes. This reaction afforded 1‐boryl‐2‐stannylalkenes with excellent regio‐ and stereoselectivity a radical mechanism. ‐addition manner is in sharp contrast to syn ‐selectivity obtained during transition metal‐catalyzed borylstannylation. The mild conditions enabled broad substrate scope, various types aromatic aliphatic were applicable. origin was elucidated DFT calculation...

The actin-binding protein p57, a member of the coronin family, is expressed in variety immune cells. It has five WD repeats and coiled-coil motif containing leucine zipper, both which are known to mediate protein–protein interactions. In order identify precise regions assess contribution these structural motifs, we prepared various truncated p57 as fusion proteins with glutathione S-transferase (GST) examined their activity. A co-sedimentation assay demonstrated that p571—371 (C-terminal...

Cyclobutenes are highly useful synthetic intermediates as well important motifs in bioactive small molecules. Herein, we report a regio-, chemo-, and enantioselective synthesis of cyclobutenes from olefins using N-sulfonyl-1,2,3-triazoles vicinal dicarbene equivalents or alkyne [2 + 2] cycloaddition surrogates. Terminal cis-olefins can be transformed into enantioenriched cyclopropanes via rhodium catalysis. Then, one pot, treatment these with tosyl hydrazide base effects diazo formation...

Plasma levels of the vascular endothelial cell markers, thrombomodulin (TM), plasminogen activator inhibitor-1 (PAI-1), tissue type (t-PA), and von Willebrand factor (vWF) were measured in 55 patients on maintenance haemodialysis (HD). TM, PAI-1 vWF antigen significantly increased before HD, but t-PA was not. Compared with antigens 1 h after HD at end HD. TM plasma decreased negatively correlated time (years) It is concluded that may cause damage increases decrease release from cells, might...

The actin-binding protein p57/coronin-1, a member of the coronin family, is selectively expressed in immune cells, and has been implicated leucocyte migration phagocytosis by virtue its interaction with F-actin (filamentous actin). We previously identified two sites N-terminal region p57/coronin-1 which it binds actin, present study we examine role leucine zipper motif located C-terminal coiled-coil domain mediating homotypic association p57/coronin-1. Recombinant solution formed homodimer,...

Phagocytosis of opsonized zymosan (OpZ) particles by differentiated cells the human leukemic cell line HL-60 induced transient periphagosomal association p57, a coronin family actin-binding protein, and F-actin with dissociation from phagosomes after ingestion was completed. Coincident OpZ ingestion, p57 phosphorylation increased transiently peaked its phagosomes. Since contains several putative sites for protein kinase C (PKC) phosphorylation, we examined effect PKC on phagosome. Purified...

We report a linear surface-electrode trap that can be used to form parallel ion strings. By adjusting the balance of radio-frequency (RF) voltages applied central and RF electrodes, pseudopotential varied from single-well double-well in radial direction. Ions located on two lines potential null are principle free excess micromotion if appropriate static applied. Calcium ions were trapped for evaluation designed electrode. An string strings observed by changing voltages. Such traps could...

Journal Article Purification and cDNA Cloning of Bovine Liver 5′-Nucleotidase, a GPI-Anchored Protein, Its Expression in COS Cells Get access Kensuke Suzuki, Suzuki *Department Microbial Chemistry, Faculty Pharmaceutical Sciences, Nagoya City UniversityTanabe-dori, Mizuho-ku, 467;**Department Developmental Biology, the National Institue for Basic BiologyOkazaki, Aichi 444 Search other works by this author on: Oxford Academic PubMed Google Scholar Yoko Furukawa, Furukawa 467; Hiro-omi Tamaua,...

CYR61 is an extracellular matrix-associated protein that promotes adhesion, migration, and proliferation of endothelial cells fibroblasts. Prostate enlargement, which frequently causes the urethral compression, often histologically observed as stromal epithelial hyperplasia in enlarged gland. To determine whether or not has relevance to progression benign prostatic (BPH), we investigated induction CYR61, also examined its function both cells.Recombinant was used for examination activity cell...

Activated protein C (APC)-protein inhibitor (PCI) complex and APC-α1antitrypsin (α1AT) levels were measured in 29 patients positive for lupus anticoagulant (LA). LA was considered if two of the following three criteria fulfilled: (1) prolongation activated partial thromboplastin time, (2) kaolin clotting time (KCT) KCT mixing test, (3) dilute Russell's viper venom (DRWT) DRWT/DR WT with high lipid concentration. Plasma thrombin-antithrombin III (AT-III) plasmin-α2-antiplasmin increased...

Abstract Visible‐light‐induced generation of α‐iminyl radicals was accomplished using α‐bromo cyclic N ‐sulfonylimines and photoredox catalysts such as erythrosine B or Ru(bpy) 3 (PF 6 ) 2 . The generated radical utilized for various reactions with allylation reagents, silyl enol ethers allenyl stannane to give the corresponding coupling products. Furthermore, atom transfer addition (ATRA) olefin proceeded provide ATRA product. This process provided a mild efficient method synthesis...

Abstract Aim To examine the effects of sonicated bacterial extracts (SBEs) from three related to periapical disease bacteria ( Porphyromonas gingivalis , P. endodontalis and F. nucleatum ) on activation matrix metalloproteinase (MMP‐2) inactivation tissue inhibitors (TIMP‐1 TIMP‐2). Methodology Each SBE was added cultures human periodontal ligament (PL) cells or HT1080 their supernatants were analysed by zymography for MMP‐2. PL cell cultures, amount TIMP‐1 determined ELISA. incubated with...