A5063194937- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Neuroendocrine regulation and behavior
- DNA and Nucleic Acid Chemistry
- Semiconductor Lasers and Optical Devices
- Photochromic and Fluorescence Chemistry
- Cannabis and Cannabinoid Research
- Memory and Neural Mechanisms
- Stress Responses and Cortisol
- Radio Frequency Integrated Circuit Design
- Sleep and Wakefulness Research
- Microwave Engineering and Waveguides
- Advanced biosensing and bioanalysis techniques
- Nicotinic Acetylcholine Receptors Study
- 3D IC and TSV technologies
- Tryptophan and brain disorders
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- Asthma and respiratory diseases
- Tracheal and airway disorders
- Photonic and Optical Devices
- CRISPR and Genetic Engineering
- VLSI and Analog Circuit Testing
Hiroshima University
2020-2025
Astellas Pharma (Japan)
2020-2024
National Institute on Alcohol Abuse and Alcoholism
2020-2024
Hokkaido University
2012-2021
Tohoku Bunka Gakuen University
2019-2021
Japan Tobacco (Japan)
1997-2019
Chitose Institute of Science and Technology
2019
Panasonic (China)
2019
SmartFactory (Germany)
2019
Kanazawa University
2001-2018
Endocannabinoids work as retrograde messengers and contribute to short-term long-term modulation of synaptic transmission via presynaptic cannabinoid receptors. It is generally accepted that the CB1 receptor (CB1) mediates effects endocannabinoid in inhibitory synapses. For excitatory synapses, however, contributions CB1, “CB3,” some other unidentified receptors have been suggested. In present study we used electrophysiological immunohistochemical techniques examined type(s) functioning at...
ABSTRACT The cyanobacterium Nostoc commune is adapted to the terrestrial environment and has a cosmopolitan distribution. In this study, role of extracellular polysaccharides (EPS) in desiccation tolerance photosynthesis N. was examined. Although photosynthetic O 2 evolution not detected desiccated colonies, ability cells evolve rapidly recovered after rehydration. air-dried colonies contained approximately 10% (wt/wt) water, field-isolated, natural with EPS were highly water absorbent...
2-Arachidonoyl-glycerol (2-AG) is an endocannabinoid that released from postsynaptic neurons, acts retrogradely on presynaptic cannabinoid receptor CB1, and induces short- long-term suppression of transmitter release. To understand the mechanisms 2-AG-mediated retrograde modulation, we investigated subcellular localization a major 2-AG biosynthetic enzyme, diacylglycerol lipase-α (DAGLα), by using immunofluorescence immunoelectron microscopy in mouse brain. In cerebellum, DAGLα was...
Magnetic ferrites such as Fe3O4 and Fe2O3 are extensively used in a range of applications because they inexpensive chemically stable. Here we show that rhodium-substituted ε-Fe2O3, ε-RhxFe2−xO3 nanomagnets prepared by nanoscale chemical synthesis using mesoporous silica template, exhibit huge coercive field (Hc) 27 kOe at room temperature. Furthermore, crystallographically oriented sample recorded an Hc value 31 kOe, which is the largest among metal-oxide-based magnets comparable to those...
The duplex-forming activity of an oligonucleotide has been photoregulated by making use the isomerization azobenzene moiety in side chain. When is isomerized from trans form to cis upon photoirradiation, melting temperature duplex between and its complementary counterpart significantly lowered, largely dissociated into two single-stranded oligonucleotides (shown schematically).
Inhibition of mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK) represents a promising strategy for the discovery new generation anticancer chemotherapeutics. Our synthetic efforts, beginning from lead compound 2, were directed at improving antiproliferative activity against cancer cells as well various drug properties. These efforts led to...
Significance Although the neurodegeneration of orexin (hypocretin)-producing neurons clearly causes sleep disorder narcolepsy, precise neural mechanisms by which prevent narcolepsy remain unclear. We previously demonstrated that inhibit cataplexy-like episodes—cataplexy is a cardinal symptom characterized sudden weakening muscle tone—via serotonin in dorsal raphe nucleus (DRN). thus used optogenetic and chemogenetic approaches to demonstrate DRN suppress episodes reducing activity amygdala...
Action potential firing or depolarization of the postsynaptic neuron can induce a transient suppression inhibitory synaptic inputs to depolarized in cerebellum and hippocampus. This phenomenon, termed depolarization-induced inhibition (DSI), is initiated postsynaptically by an elevation intracellular Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) expressed presynaptically as transmitter release. It is, therefore, thought that some retrograde signal must exist from neurons...
A drop in melting point of 21.5°C is induced by the UV-photolytic trans→cis isomerization duplex formed between an oligonucleotide bearing two D-threoninol-tethered azobenzene moieties side chain and its complementary counterpart. On irradiation with visible light, dissociated single-stranded oligonucleotides regenerate duplex.
P-cadherin is a subclass of Ca2+-dependent cell-cell adhesion molecules present in mouse placenta, where its localization suggests function connecting the embryo to uterus (Nose, A., and M. Takeichi. 1986. J. Cell Biol. 103:2649-2658). We recently identified human cadherin detected by an mAb capable disrupting A-431 cells, found that it was closely related immunochemically P-cadherin. Curiously, this undetectable placenta immunohistochemical examination (Shimoyama, Y., S. Hirohashi, Hirano,...
2-Arachidonoylglycerol (2-AG) is the endocannabinoid that mediates retrograde suppression of synaptic transmission in brain. 2-AG synthesized activated postsynaptic neurons by sn -1-specific diacylglycerol lipase (DGL), binds to presynaptic cannabinoid CB 1 receptors, suppresses neurotransmitter release, and degraded mainly monoacylglycerol (MGL). In basolateral amygdala complex, it has been demonstrated particularly enriched axon terminals cholecystokinin (CCK)-positive GABAergic...
The duplex-forming activities of oligonucleotides can be photomodulated by incorporation an azobenzene unit. Upon isomerizing the trans-azobenzene to cis form irradiation with UV light, Tm value duplex (with complementary DNA) is lowered so that dissociated. formed again when cis-azobenzene converted visible light. photoregulation successful irrespective position unit in oligonucleotides. intercalates between two DNA base pairs duplexes and stabilizes them because a favorable enthalpy...
Rab3A small G protein is a member of the Rab family and most abundant in brain, where it localized on synaptic vesicles. Evidence accumulating that plays key role neurotransmitter release plasticity. cycles between GDP-bound inactive GTP-bound active forms, this change activity associated with trafficking cycle vesicles at nerve terminals. Rab3 GTPase-activating (GAP) stimulates GTPase expected to determine timing dissociation from vesicles, which may be coupled vesicle exocytosis. GAP...
Smoking may have multifactorial effects on asthma phenotypes, particularly in severe asthma. Cluster analysis has been applied to explore novel which are not based any a priori hypotheses.To phenotypes by cluster when including smoking patients with asthma.We recruited total of 127 subjects asthma, 59 current or ex-smokers, from our university hospital and its 29 affiliated hospitals/pulmonary clinics. Clinical variables obtained during 2-day stay were used for analysis. After clustering...
Abstract Empathy is a cognitive and emotional response that occurs in individuals who observe the experiences of others. In this study, we aimed to understand neural circuit mechanisms involved empathy by using observational fear learning (OFL) test, immunohistochemical (IHC) patch-clamp (PC) analyses mice. We labeled pyramidal cells dorsal medial prefrontal cortex (dmPFC) project basolateral lateral amygdala (BLA) or periaqueductal gray (PAG) control its associated behaviors, respectively,...
SUMMARY Changes in photosynthetic activity and trehalose levels field‐isolated, natural colonies of the terrestrial cyanobacterium Nostoc commune responding to desiccation salt stress were investigated. As water content decreased N. during desiccation, O 2 ‐evolving no was detected desiccated colonies. A high level evolution restored as they absorbed atmospheric moisture, indicating that only a small amount is required for reactivation photosynthesis. No detectable found fully hydrated...
A small molecule compound, JTP-74057/GSK1120212/trametinib, had been discovered as a very potent antiproliferative agent able to induce the accumulation of CDK inhibitor p15INK4b. To conduct its drug development rationally an anticancer agent, molecular targets this compound were identified MEK1/2 using compound-affinity chromatography. It was shown that JTP-74057 directly bound MEK1 and MEK2 allosterically inhibited their kinase activities, inhibitory characteristics similar those known...
The serotonergic (5-HTergic) system arising from the dorsal raphe nucleus (DRN) is implicated in various physiological and behavioral processes, including stress responses. DRN comprised of several subnuclei, serving specific functions with distinct afferent efferent connections. Furthermore, subsets 5-HTergic neurons are known to coexpress other transmitters, GABA, glutamate, or neuropeptides, thereby generating further heterogeneity. However, despite growing evidence for functional...
Abstract From the viewpoints of large capacity, long‐term guarantee, and low cost, interest in magnetic recording tapes has undergone a revival as an archive storage media for big data. Herein, we prepared new series metal‐substituted ϵ‐Fe 2 O 3 , ϵ‐Ga III 0.31 Ti IV 0.05 Co II Fe 1.59 nanoparticles with average size 18 nm. Ga, Ti, cations tune properties to specifications demanded tape. The coercive field was tuned 2.7 kOe by introduction single‐ion anisotropy on ( S =3/2) along c‐axis....