A5002574645- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Aquaculture Nutrition and Growth
- Hypothalamic control of reproductive hormones
- Fish Ecology and Management Studies
- Reproductive Biology and Fertility
- Estrogen and related hormone effects
- Fish Biology and Ecology Studies
- Retinoids in leukemia and cellular processes
- MicroRNA in disease regulation
- Hormonal and reproductive studies
- Physiological and biochemical adaptations
- Neuropeptides and Animal Physiology
- Molecular Biology Techniques and Applications
- Renal and related cancers
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Sexual Differentiation and Disorders
- CRISPR and Genetic Engineering
- Aquaculture disease management and microbiota
- Birth, Development, and Health
- Fish biology, ecology, and behavior
- Reproductive Health and Technologies
Fish Physiology and Genomics Institute
2016-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2025
Genopole (France)
2009-2024
Institut National de la Recherche Agronomique
2001-2015
Université de Rennes
2003-2015
Structure Fédérative de Recherche en Biologie et Santé de Rennes
2015
Cancéropôle Grand Ouest
2014
Institut de Génomique Fonctionnelle
2011-2012
Institut thématique Génétique, génomique et bioinformatique
2011-2012
Biogen (Belgium)
2011-2012
In many eukaryotes, meiotic recombination occurs preferentially at discrete sites, called hotspots. various lineages, hotspots are located in regions with promoter-like features and evolutionarily stable. Conversely, some mammals, driven by PRDM9 that targets away from promoters. Paradoxically, induces the self-destruction of its this triggers an ultra-fast evolution mammalian is ancestral to all animals, suggesting a critical importance for program, but has been lost lineages surprisingly...
Abstract Early expression of estrogen receptors (esr) and their role in regulating early cyp19a1b encoding brain aromatase were examined the zebrafish. Using toto hybridization quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR), a significant increase esr1, esr2a, esr2b was observed between 24 48 hours postfertilization (hpf). In demonstrated that esr2a esr2b, but not are found hypothalamus. real‐time RT‐PCR, an mRNAs occurs hpf, indicating is temporally correlated with...
The general rules established from mammalian species for the regulation of spermatogenesis by gonadotropins may not be fully relevant in fish. Particularly, Fsh is as potent Lh to stimulate steroidogenesis and receptor expressed Leydig cells. In seasonal breeders, likely major gonadotropin involved onset required support progression gamete release. However, genes that relay action have been poorly investigated present study was aimed at identifying gonadotropin-dependent testis during fish...
Continuous or cyclic production of spermatozoa throughout life in adult male vertebrates depends on a subpopulation undifferentiated germ cells acting as spermatogonial stem (SSCs). What makes these self-renew differentiate is barely understood, particular nonmammalian species, including fish. In the highly seasonal rainbow trout, at end annual spermatogenetic cycle, tubules spawning testis contain only spermatozoa, with exception scarce spermatogonia that remain tubular wall and will...
With the exception of modern mammals, most vertebrate species possess two GnRH genes, GnRH-1 and GnRH-2. In addition, in many teleost fish, there is a third gene called GnRH-3. If main function unambiguously to stimulate gonadotropin release, other forms still lack clear functions. This particularly true for highly conserved GnRH-2 that encodes chicken GnRH-II. variant consistently expressed neurons dorsal synencephalon groups but has no functions supported by anatomical, pharmacological,...
Spermatogenesis is a late developmental process that involves coordinated expression program in germ cells and permanent communication between the testicular somatic germ-line. Current knowledge regarding molecular factors driving male cell proliferation differentiation vertebrates still limited mainly based on existing data from rodents human. Fish with marked reproductive cycle development synchronous cysts have proven to be choice models study precise stages of spermatogenetic...
Thegonadal soma-derived factor (GSDF) is a new member of the transforming growth beta (TGF-beta) superfamily that regulates proliferation primordial germ cells (PGC) in developing embryos and spermatogonia juvenile male trout. The gsdf transcripts are expressed somatic supporting cell development. In zebrafish, we show GSDF encoded by single copy gene generates polymorphic proteins. We determined expression occurs before gonadal differentiation restricted to gonads. Gene maintained adult...
Abstract In many eukaryotes, meiotic recombination occurs preferentially at discrete sites, called hotspots. various lineages, hotspots are located in regions with promoter-like features and evolutionarily stable. Conversely, some mammals, driven by PRDM9 that targets away from promoters. Paradoxically, induces the self-destruction of its this triggers an ultra-fast evolution mammalian is ancestral to all animals, suggesting a critical importance for program, but has been lost lineages...
Genomic clones containing the gene for glutathione peroxidase-like androgen-regulated murine epididymal protein of 24 kilodaltons (arMEP24) were isolated. A 9-kilobase DNA fragment was sequenced and found to contain entire coding region gene, which is divided into five exons. The exact sizes boundaries exon blocks deduced by comparison with cDNA sequence. One major four weak transcription initiation sites in epididymis localized primer extension. promoter does not a conventional TATA box...
In vertebrates, gonadotropins (GTHs) (FSH and LH) are two circulating pituitary glycoprotein hormones that play a major role in the regulation of gonadal functions, including cell proliferation/differentiation steroidogenesis. mammals, it is well known their biological effects mediated by highly specific membrane-bound receptors expressed preferentially on somatic cells gonads. However, fish, binding functional studies have shown cross-reactivity may occur GTH depending species. To...
Hexachlorobenzene (HCB) is a persistent environmental contaminant that has the potential to interfere with steroid hormone regulation. The prostate requires precise control by androgens regulate its growth and function. To determine if HCB impacts androgen action in prostate, we used number of methods. Our vitro cell-culture-based assay firefly luciferase reporter gene driven an androgen-responsive promoter. In presence dihydrotestosterone, low concentrations (0.5-5 nM) increased production...
The capacity of testicular somatic cells to promote and sustain germ cell differentiation is largely regulated by sexual steroids notably androgens. In fish species the importance androgens emphasized their ability induce sex reversal developing fries trigger spermatogenesis. Here we studied influence on gene expression in trout testis using microarrays. Following treatment immature males with physiological doses testosterone or 11-ketotestosterone, 418 genes that exhibit changes were...
What makes the spermatogonial stem cells (SSCs) self-renew or differentiate to produce spermatozoa is barely understood, in particular nonmammalian species. Our research explores possible regulations of SSC niche teleost, locally by paracrine factors and peripherally hormonal regulation. In present study, we focus on Gdnf-Gfra1 pathway that plays a major role regulation self-renewal mammals. We describe complex evolution genes encoding for Gdnf Gfra1 proteins trout with emergence three gdnf...