A5061849198- PI3K/AKT/mTOR signaling in cancer
- Nanoplatforms for cancer theranostics
- Computational Drug Discovery Methods
- Glioma Diagnosis and Treatment
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Cancer Cells and Metastasis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Cell Image Analysis Techniques
- Chemical Reactions and Isotopes
- Aquaculture Nutrition and Growth
- Fish Ecology and Management Studies
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Aquaculture disease management and microbiota
- Cancer Research and Treatments
- Synthesis and biological activity
- Brain Metastases and Treatment
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- 3D Printing in Biomedical Research
- Caveolin-1 and cellular processes
- RNA Research and Splicing
Norwegian Institute of Marine Research
2014-2024
University of Bergen
2003-2017
Haukeland University Hospital
2006-2015
University of Tübingen
2015
Goethe University Frankfurt
2015
Luxembourg Institute of Health
2015
Ålesund Hospital
2013
Czech Academy of Sciences, Institute of Macromolecular Chemistry
2013
Czech Academy of Sciences, Institute of Experimental Medicine
2013
Charles University
2013
Abstract Introgression of farmed salmon escapees into wild stocks is a major threat to the genetic integrity populations. Using germ cell-free fish in aquaculture may mitigate this problem. Our study investigated whether it possible produce F0 by using CRISPR-Cas9 knock out dnd , factor required for cell survival vertebrates. To avoid studying mosaic animals, sgRNA targeting alb was simultaneously used as visual tracer since phenotype KO complete loss pigmentation. Induced mutations ( ) and...
Understanding the biological function behind key proteins is of great concern in Atlantic salmon, both due to a high commercial importance and an interesting life history. Until recently, functional studies salmonids appeared be difficult. However, recent discovery targeted mutagenesis using CRISPR/Cas9 (clustered regularly interspaced palindromic repeats/CRISPR-associated) system enables performing salmon extent. We used target two genes involved pigmentation, tyrosinase (tyr) solute...
Angiogenesis is regarded as a hallmark of cancer progression and it has been postulated that solid tumor growth depends on angiogenesis. At present, however, clear cell invasion can occur without angiogenesis, phenomenon particularly evident by the infiltrative malignant brain tumors, such glioblastomas (GBMs). In these amplification or overexpression wild-type (wt) truncated constitutively activated epidermal factor receptor (EGFR) are important events in GBM development, where complex...
// Aurélie Poli 1,2,* , Jian Wang 1,* Olivia Domingues 2 Jesús Planagumà 1 Tao Yan 1,6 Cecilie Brekke Rygh Kai Ove Skaftnesmo Frits Thorsen Emmet McCormack 3 François Hentges Paal Henning Pedersen 4 Jacques Zimmer Per Øyvind Enger 1,4 and Martha Chekenya 1,5 Translational Cancer Research, Department of Biomedicine, University Bergen, Norway Laboratoire d’Immunogénétique-Allergologie, CRP-Santé, Luxembourg Medicine, Haukeland...
Early puberty poses a significant challenge for male Atlantic salmon in aquaculture due to its negative impact on growth and welfare. The regulation of vertebrates involves 2 key reproductive hormones: follicle-stimulating hormone (FSH) luteinizing (LH) their gonadal receptors. In mice lacking FSH receptor, testes size is reduced, but fertility maintained, while medaka zebrafish with disrupted fshr gene exhibit near normal testis fertility. these fishes both Fsh Lh are present during may...
Aberrant expression of the progenitor marker Neuron-glia 2 (NG2/CSPG4) or melanoma proteoglycan on cancer cells and angiogenic vasculature is associated with an aggressive disease course in several malignancies including glioblastoma multiforme (GBM) melanoma. Thus, we investigated mechanism NG2 mediated malignant progression its potential as a therapeutic target clinically relevant GBM animal models. Xenografting overexpressing cell lines resulted increased growth rate, angiogenesis...
Expression of activity-regulated cytoskeleton associated protein (Arc) is crucial for diverse types experience-dependent synaptic plasticity and long-term memory in mammals. However, the mechanisms governing Arc-specific translation are little understood. Here, we asked whether Arc regulated by microRNAs. Bioinformatic analysis predicted numerous candidate miRNA binding sites within 3'-untranslated region (UTR). Transfection corresponding microRNAs human embryonic kidney cells inhibited...
Genetic introgression of escaped farmed Atlantic salmon (Salmo salar) into wild populations is a major environmental concern for the aquaculture industry. Using sterile fish in commercial operations is, therefore, sustainable strategy bio-containment. So far, only commercially used methodology producing triploidization. However, triploid are less robust. A novel approach which to achieve sterility produce germ cell-free salmon, can be accomplished by knocking out dead-end (dnd) gene using...
Although CD133 has been proposed as a marker for brain tumor-initiating cells, studies show that tumorigenic potential exists among CD133(-) glioma cells well. However, it is not established whether the ability of to form tumors property confined small subpopulation, rather than common trait associated with most cell types. Thus, we used lentiviral vectors expressing green fluorescent protein under lineage-specific promoters identify Nestin, glial fibrillary acidic (GFAP), and...
Intercellular communication between cancer cells, especially and stromal plays an important role in disease progression. We examined the intercellular transfer of organelles proteins vitro vivo tunneling nanotubes (TNTs) this process. TNTs are membrane bridges that facilitate unclear origin. Using 3-dimensional quantitative qualitative confocal microscopy, we showed contain green fluorescent protein (GFP)-early endosome antigen (EEA) 1, GFP Rab5, Rab11, Rab8, transferrin (Tf), Tf receptor...
Our understanding of the molecular mechanisms implementing pubertal maturation testis in vertebrates is incomplete. This topic relevant Atlantic salmon aquaculture, since precocious male puberty negatively impacts animal welfare and growth. We hypothesize that certain miRNAs modulate mRNAs for initiation puberty. To explore which regulate during salmon, we performed an integrated transcriptome analysis (miRNA mRNA-seq) at three stages development: immature, long-term quiescent stage, a...
Vgll3 is linked to age at maturity in Atlantic salmon (Salmo salar). However, the molecular mechanisms involving controlling timing of puberty as well relevant tissue and cell types are currently unknown. associated Hippo pathway has been reduced proliferation activity different tissues. Analysis gene expression reveals for first time that vgll3 several members were down-regulated testis during onset remained repressed maturing testis. In gonads, we found Sertoli granulosa cells males...
Glioblastoma is the most frequent and malignant primary brain tumor with a poor prognosis. The translation of therapeutic strategies for glioblastoma from experimental phase into clinic has been limited by insufficient animal models, which lack important features human tumors. Lentiviral gene therapy an attractive option glioblastoma, we validated in clinically relevant model.We used rodent xenograft model that recapitulates invasive angiogenic to analyze transduction pattern efficacy...
Abstract Background Expression of neuronal elements has been identified in various glial tumors, and glioblastomas (GBMs) with differentiation patterns have reportedly associated longer survival. However, the class III β-tubulin linked to increasing malignancy astrocytomas. Thus, significance markers gliomas is not established. Methods The expressions β-tubulin, neurofilament protein (NFP), microtubule-associated 2 (MAP2) neuron-specific enolase (NSE) were investigated five GBM cell lines...
Biologic and therapeutic advances in melanoma brain metastasis are hampered by the paucity of reproducible predictive animal models. In this work, we developed a robust model that empowers quantitative tracking cellular dissemination tumor progression. Human cells labeled with superparamagnetic iron oxide nanoparticles (SPION) were injected into left cardiac ventricle mice visualized MRI. We showed SPION exposure did not affect viability, growth, or migration multiple cell lines across...
Although several studies suggest that stromal fibroblasts mediate treatment resistance in cancer types, little is known about how tumor-associated astrocytes modulate the response brain tumors. Since traditionally used metabolic assays do not distinguish activity between and tumor cells, since 2-dimensional co-culture system does recreate formidable complexity of microenvironment within 3-dimensional structures such as solid tissue, we instead established a glioblastoma (GBM) cell-specific...
Our goal was to develop strategies quantify the accumulation of model therapeutics in small brain metastases using multimodal imaging, order enhance potential for successful treatment. Human melanoma cells were injected into left cardiac ventricle immunodeficient mice. Bioluminescent, MR and PET imaging applied evaluate limits detection contrast agent extravasation metastases. A pharmacokinetic estimate vascular permeability. Bioluminescent after injecting d-luciferin (molecular weight (MW)...
Melanoma patients carry a high risk of developing brain metastases, and improvements in survival are still measured weeks or months. Durable disease control within the is impeded by poor drug penetration across blood-brain barrier, as well intrinsic acquired resistance. Augmented mitochondrial respiration key resistance mechanism BRAF-mutant melanomas but, we show this study, dependence on may also be exploited therapeutically. We first used high-throughput pharmacogenomic profiling to...
Abstract In all vertebrates studied so far, germ cells are not required for pubertal maturation of the gonadal steroidogenic system, subsequent development secondary sex characteristics and reproductive behavior. To explore if absence affects puberty or growth in Atlantic salmon, cell-free (GCF), dnd knockout wild type (WT) postsmolts were stimulated to enter puberty. No GCF fish entered puberty, whereas 66.7% (males) 30% (females) WT completed respectively. Expression genes related...
Abstract Precise gene editing such as CRISPR/Cas9-mediated homology directed repair (HDR) can increase our understanding of function and improve traits importance for aquaculture. This fine-tuned technology has not been developed farmed fish including Atlantic salmon. We performed knock-in (KI) a FLAG element in the slc45a2 salmon using sense (S), anti-sense (AS) double-stranded (ds) oligodeoxynucleotide (ODN) templates with short (24/48/84 bp) arms. show vivo ODN integration almost all...