A5079021093- Cellular transport and secretion
- Nanoparticles: synthesis and applications
- interferon and immune responses
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Receptor Mechanisms and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Nanoparticle-Based Drug Delivery
- Infant Nutrition and Health
- Supramolecular Self-Assembly in Materials
- Selenium in Biological Systems
- HIV Research and Treatment
- Genetic and Kidney Cyst Diseases
- Complement system in diseases
- Advanced Nanomaterials in Catalysis
- Vector-Borne Animal Diseases
- Prion Diseases and Protein Misfolding
- Rabbits: Nutrition, Reproduction, Health
- Biochemical and Structural Characterization
- Influenza Virus Research Studies
- Neuropeptides and Animal Physiology
- Mercury impact and mitigation studies
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Immune Response and Inflammation
Institut Cochin
2005-2024
University of Bergen
2015-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2024
Génétique Animale et Biologie Intégrative
2022
Inserm
2005-2009
Institut Pasteur
2009
Centre National de la Recherche Scientifique
2005-2008
Université Paris Cité
2005-2008
Délégation Paris 5
2005-2006
Adaptor protein complex 2 alpha and beta-appendage domains act as hubs for the assembly of accessory networks involved in clathrin-coated vesicle formation. We identify a large repertoire interactors by mass spectrometry. These interact with two distinct ligand interaction sites on (the "top" "side" sites) that bind motifs from those previously identified alpha-appendage. solved structure peptide Eps15 bound to side site cargo adaptor beta-arrestin top site. show proteins can simultaneously...
Arrestins are important proteins, which regulate the function of serpentine heptahelical receptors and contribute to multiple signaling pathways downstream receptors. The ubiquitous beta-arrestins believed exclusively as monomers, although self-association is assumed control activity visual arrestin in retina, where this isoform particularly abundant. Here oligomerization status was investigated using different approaches, including co-immunoprecipitation epitope-tagged resonance energy...
Intercellular communication between cancer cells, especially and stromal plays an important role in disease progression. We examined the intercellular transfer of organelles proteins vitro vivo tunneling nanotubes (TNTs) this process. TNTs are membrane bridges that facilitate unclear origin. Using 3-dimensional quantitative qualitative confocal microscopy, we showed contain green fluorescent protein (GFP)-early endosome antigen (EEA) 1, GFP Rab5, Rab11, Rab8, transferrin (Tf), Tf receptor...
RIG-I is a pivotal receptor that detects numerous RNA and DNA viruses. Thus, its defectiveness may strongly impair the host antiviral immunity. Remarkably, very little information available on single-nucleotide polymorphisms (SNPs) presenting functional impact response.Here, we studied all non-synonymous SNPs of using biochemical structural modeling approaches. We identified two important variants: (i) frameshift mutation (P(229)fs) generates truncated, constitutively active (ii) serine to...
The HIV‐1 Nef protein perturbs the trafficking of membrane proteins such as CD4 by interacting with clathrin–adaptor complexes. We previously reported that alters early/recycling endosomes, but its role at plasma is poorly documented. Here, we used total internal reflection fluorescence microscopy, which restricts analysis to a ∼100 nm region adherent surface cells, focus on dynamic relative clathrin. colocalized both clathrin spots (CS) remained static cell surface, corresponding...
Background The primary cilium is a sensory organelle generated from the centrosome in quiescent cells and found at surface of most cell types, where it controls important physiological processes. Specific sets membrane proteins involved sensing extracellular milieu are concentrated within cilia, including G protein coupled receptors (GPCRs). Most GPCRs regulated by β-arrestins, βarr1 βarr2, which control both their signalling endocytosis, suggesting that βarrs may also function cilium....
Beta‐arrestins (βarrs) play a central role in the regulation of G‐protein‐coupled receptors (GPCRs). Their binding to phosphorylated activated GPCRs induces conformational transition an active state resulting release their flexible C‐terminal tail. Binding sites for clathrin and adaptor protein (AP)‐2 complex are then unmasked, which drive recruitment βarrs‐GPCR complexes into clathrin‐coated pits (CCPs). A conserved isoleucine‐valine‐phenylalanine (IVF) motif tail controls βarr activation...
Cellular organelles have been shown to shuttle between cells in co-culture. We hereby show that titanium dioxide (TiO2) nanoparticles (NPs) can be transferred such a manner, direct contact, along with endosomes and lysosomes. A co-culture system was employed for this purpose the NP transfer observed mammalian including normal rat kidney (NRK) HeLa cells. found small GTPase Arf6 facilitates intercellular of smaller NPs agglomerates. Spherical, anatase nano-TiO2 sizes 5 (Ti5) 40 nm (Ti40) were...
Abstract Co ntact- de pendent i ntercellular t ransfer (codeIT) of cellular constituents can have functional consequences for recipient cells, such as enhanced survival and drug resistance. Pathogenic viruses, prions bacteria also utilize this mechanism to spread adjacent cells potentially evade immune detection. However, little is known about the molecular underlying intercellular transfer process. Here, we present a novel microscopy-based screening method identify regulators cargo codeIT....
The sustainability of mammals on Earth relies milk. During lactation, maternal exposure to pollutants like metal nanoparticles (NPs) can affect offspring development and survival. Despite being banned from food applications in Europe due suspected toxicity, titanium dioxide (TiO2) NPs are still massively manufactured for countless other uses. While contamination ecosystems is well documented, remains underexplored. Here, we used synchrotron X-ray fluorescence single particle inductively...