A5046946065- Advanced Fluorescence Microscopy Techniques
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Cellular transport and secretion
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cellular Mechanics and Interactions
- Autophagy in Disease and Therapy
- bioluminescence and chemiluminescence research
- RNA modifications and cancer
- Spectroscopy Techniques in Biomedical and Chemical Research
- Mitochondrial Function and Pathology
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Advanced Biosensing Techniques and Applications
- Digital Imaging for Blood Diseases
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Biotin and Related Studies
- Genetics, Aging, and Longevity in Model Organisms
- Protein Degradation and Inhibitors
- Receptor Mechanisms and Signaling
- Image Processing Techniques and Applications
Institut de génétique et de développement de Rennes
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Université de Rennes
2015-2024
Structure Fédérative de Recherche en Biologie et Santé de Rennes
2017-2022
Inserm
2017-2021
Technologie Médicale (France)
2021
Université Paris Cité
2005-2019
Institut Jacques Monod
2004-2019
Canadian Nautical Research Society
2018
Imaging Center
2017
Abstract Fluorescent protein‐based FRET is a powerful method for visualizing protein–protein interactions and biochemical reactions in living cells. It can be difficult, however, to avoid photobleaching when observing fluorescent cells under the microscope, especially those expressing CFP. We compared sensitivity of two pairs light‐induced fluorescence changes donor, on determination by lifetime imaging microscopy (FLIM). Thanks very low excitation light levels time‐ space‐correlated single...
Many epithelial cancers show cell cycle dysfunction tightly correlated with the overexpression of serine/threonine kinase Aurora A (AURKA). Its role in mitotic progression has been extensively characterised, and evidence for new AURKA functions emerges. Here, we reveal that is located imported mitochondria several human cancer lines. Mitochondrial impacts on two organelle functions: mitochondrial dynamics energy production. When expressed at endogenous levels during interphase, it induces...
Biocompatible fluorescent reporters with spectral properties spanning the entire visible spectrum are indispensable tools for imaging biochemistry of living cells and organisms in real time. Here, we report engineering a chemogenetic reporter tunable optical properties. A collection fluorogenic chromophores various electronic enables to generate bimolecular assemblies that cover from blue red using single protein tag engineered optimized by directed evolution rational design. The ability...
ABSTRACT Human herpesvirus 8 is associated with all forms of Kaposi's sarcoma, AIDS-associated body cavity-based lymphomas, and some multicentric Castleman's disease. Herpesvirus 8, like other gammaherpesviruses, can establish a latent infection in which viral genomes are stably maintained as multiple episomes. The nuclear antigen (LANA or LNAI) may play an essential role the stable maintenance episomes, notably by interacting concomitantly metaphase chromosomes, thus ensuring efficient...
Hutchinson–Gilford progeria syndrome (HGPS) is a dominant autosomal premature aging caused by the expression of truncated prelamin A designated progerin (Pgn). A-type and B-type lamins are intermediate filament proteins that polymerize to form nuclear lamina network apposed inner membrane vertebrate somatic cells. It not known if in vivo both type assemble independently or co-assemble. The blebbing disorganization envelope adjacent heterochromatin cells from patients with HGPS hallmark...
Abstract Overexpression of AURKA is a major hallmark epithelial cancers. It encodes the multifunctional serine/threonine kinase aurora A, which activated at metaphase and required for cell cycle progression; assessing its activation in living cells mandatory next-generation drug design. We describe here Förster’s resonance energy transfer (FRET) biosensor detecting conformational changes A induced by autophosphorylation on Thr288. The functionally replaces endogenous allows to be followed...
Arrestins are important proteins, which regulate the function of serpentine heptahelical receptors and contribute to multiple signaling pathways downstream receptors. The ubiquitous beta-arrestins believed exclusively as monomers, although self-association is assumed control activity visual arrestin in retina, where this isoform particularly abundant. Here oligomerization status was investigated using different approaches, including co-immunoprecipitation epitope-tagged resonance energy...
Oils containing both fluorescent semiconductor and magnetic oxide nanoparticles are used to produce oil in water emulsions. This technique produces droplets with homogeneous fluorescence high nanoparticle concentrations. The optical properties of the studied as a function droplet sizes for various concentrations nanoparticles. For all tested, we find linear variation intensity volume. given size quantum dot (QD) concentration, drops sharply concentration. We show that this decrease is due...
Amphiphysin 1, an endocytic adaptor concentrated at synapses that couples clathrin-mediated endocytosis to dynamin-dependent fission, was also shown have a regulatory role in actin dynamics. Here, we report amphiphysin 1 interacts with N-WASP and stimulates N-WASP- Arp2/3-dependent polymerization. Both the Src homology 3 N-BAR domains are required for this stimulation. Acidic liposome-triggered, N-WASP-dependent polymerization is strongly impaired brain cytosol of knock-out mice. FRET-FLIM...
Reorganization of the nuclear machinery after mitosis is a fundamental but poorly understood process. Here, we investigate recruitment nucleolar processing proteins in nucleolus living cells at time nucleus formation. We question role prenucleolar bodies (PNBs), during migration from chromosome periphery to sites rDNA transcription. Surprisingly, early and late pass through same PNBs as demonstrated by rapid two-color four-dimensional imaging quantification, whereas different order protein...
β-arrestins (β-arrs), two ubiquitous proteins involved in serpentine heptahelical receptor regulation and signaling, form constitutive homo- heterooligomers stabilized by inositol 1,2,3,4,5,6-hexakisphosphate (IP6). Monomeric β-arrs are believed to interact with receptors after agonist activation, therefore, β-arr oligomers have been proposed represent a resting biologically inactive state. In contrast this, we report here that the interaction subsequent titration out of nucleus...
Förster Resonance Energy Transfer (FRET) measured with Fluorescence Lifetime Imaging Microscopy (FLIM) is a powerful technique to investigate spatio-temporal regulation of protein-protein interactions in living cells. When using standard fitting methods analyze time domain FLIM, the correct estimation FRET parameters requires high number photons and therefore long acquisition times which are incompatible observation dynamic interactions. Recently, non-fitting strategies have been developed...
Abstract Monitoring of different signalling enzymes in a single assay using multiplex biosensing provides multidimensional workspace to elucidate biological processes, pathway crosstalk, and determine precise sequence events at the living cell level. In this study, we interrogate complexity cAMP/PKA-MAPK/ERK1&2 crosstalk by multi-parameter experiments correlate biochemical activities simultaneously time space. Using excitation wavelength dual colour FLIM method are able detect...
Epithelial and haematologic tumours often show the overexpression of serine/threonine kinase AURKA. Recently, AURKA was shown to localise at mitochondria, where it regulates mitochondrial dynamics ATP production. Here we define molecular mechanisms in regulating turnover by mitophagy. triggers degradation Inner Mitochondrial Membrane/matrix proteins interacting with core components autophagy pathway. On inner membrane, forms a tripartite complex MAP1LC3 mitophagy receptor PHB2, which...
Although several mechanisms of macroautophagy/autophagy have been dissected in the last decade, following this pathway real time remains challenging. Among early events leading to its activation, ATG4B protease primes key autophagy player MAP1LC3B/LC3B. Given lack reporters follow event living cells, we developed a Förster's resonance energy transfer (FRET) biosensor responding priming LC3B by ATG4B. The was generated flanking within pH-resistant donor-acceptor FRET pair,...