A5091097806- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
- Antibiotics Pharmacokinetics and Efficacy
- Neuroscience and Neuropharmacology Research
- Immune Cell Function and Interaction
- Microtubule and mitosis dynamics
- Circadian rhythm and melatonin
- Photoreceptor and optogenetics research
- Erythrocyte Function and Pathophysiology
- Amino Acid Enzymes and Metabolism
- Antifungal resistance and susceptibility
- Pharmacogenetics and Drug Metabolism
- Pancreatic function and diabetes
- Immune Response and Inflammation
- Antibiotic Resistance in Bacteria
- Renal Diseases and Glomerulopathies
- Sleep and Wakefulness Research
- Neuroendocrine regulation and behavior
- Complement system in diseases
- Mycobacterium research and diagnosis
- Drug Transport and Resistance Mechanisms
- Ion channel regulation and function
- Carcinogens and Genotoxicity Assessment
- Renin-Angiotensin System Studies
Okayama University
2016-2025
Japanese Red Cross Nagoya Daini Hospital
2004-2024
Chiba University
2023
Red Cross Hospital
2009-2021
Japan Science and Technology Agency
2015-2016
University of Oklahoma Health Sciences Center
2015
Fujisawa City Hospital
2014-2015
Osaka City General Hospital
2006-2013
Osaka Police Hospital
2011-2012
Higashi Takarazuka Satoh Hospital
2012
Inhibition of sodium glucose cotransporter 2 (SGLT2) has been reported as a new therapeutic strategy for treating diabetes. However, the effect SGLT2 inhibitors on kidney is unknown. In addition, whether have an anti-inflammatory or antioxidative stress still unclear. this study, to resolve these issues, we evaluated effects inhibitor, dapagliflozin, using mouse model type diabetes and cultured proximal tubular epithelial (mProx24) cells. Male db/db mice were administered 0.1 1.0 mg/kg...
Klotho mutant mouse (kl-/-), a model for human aging, exhibits various phenotypes in wide range of organs including arteriosclerosis, neural degeneration, skin and gonadal atrophy, pulmonary emphysema, calcification soft tissues, cognition impairment. mRNA, however, is expressed only brain, kidney, reproductive organs, pituitary gland, parathyroid gland. Therefore it remains to be elucidated how lack protein these limited leads the variety phenotypes. To shed light on mechanisms by which...
The effects of sesamin, a lignan from sesame oil, on various aspects cholesterol metabolism were examined in rats maintained dietary regimens. When given at level 0.5% for 4 weeks, sesamin reduced the concentration serum and liver significantly irrespective presence or absence diet, except one experiment which purified diet free was given. On feeding there decrease lymphatic absorption accompanying an increase fecal excretion neutral, but not acidic, steroids, particularly when...
Rat pancreatic AR42J cells possess exocrine and neuroendocrine properties. Activin A induces morphological changes converts them into neuron-like cells. In activin-treated cells, mRNA for polypeptide (PP) but not that either insulin or glucagon was detected by reverse transcription-PCR. About 25% of the were stained anti-PP antibody. When incubated with betacellulin, a small portion positively antiinsulin antibodies. The effect betacellulin dose dependent, being maximal at 2 nM....
Bacterial DNA contains immunostimulatory CpG motifs that trigger an innate immune response capable of promoting host survival following infectious challenge. Yet CpG-driven activation may also have deleterious consequences, ranging from autoimmune disease to death. We find repetitive elements present at high frequency in mammalian telomeres, but rare bacteria, down-regulate CpG-induced activation. Suppressive activity correlates with the ability telomeric TTAGGG repeats form G-tetrads....
Angiotensin converting enzyme (ACE) was localized in rat heart by quantitative vitro autoradiography with 125I-351A as the radioligand. The binding association constant (KA) of radioligand measured membrane-rich fractions atrium, ventricle, and lung a radioinhibitor assay. A single class high-affinity sites detected each tissue, significant difference found between KA values for atria ventricles rank order greater than lungs ventricles. For autoradiography, coronal sections (10 micron)...
Background: Telomeres are specific structures located at the ends of chromosomes that help maintain chromosome stability. In most tissues, telomeres become shorter as cells divide, a phenomenon thought to be associated with limitations on normal cell proliferation. Almost all types cancer cells, including bladder express enzyme telomerase, which can or extend telomere length. Purpose: We examined telomerase activity in tumor specimens from cohort patients and determined whether could...
l-Glutamate is believed to function as an intercellular transmitter in the islets of Langerhans. However, critical issues, <i>i.e.</i> where, when and howl-glutamate appears, what happens upon stimulation glutamate receptors islets, remain unresolved. Vesicular transporter 2 (VGLUT2), isoform vesicular essential for neuronal storage l-glutamate, expressed α cells (Hayashi, M., Otsuka, Morimoto, R., Hirota, S., Yatsushiro, Takeda, J., Yamamoto, A., Moriyama, Y. (2001) <i>J. Biol. Chem.</i>...
Abstract It is unclear whether the improvement in diabetic nephropathy by sodium glucose cotransporter 2 ( SGLT 2) inhibitors caused a direct effect on or hyperglycemia. Here, we investigated of dapagliflozin early‐stage using mouse model type 1 diabetes and murine proximal tubular epithelial cells. Eight‐week‐old Akita mice were treated with insulin for 12 weeks. Body weight, urinary albumin excretion, blood pressure, as well levels hemoglobin A1c measured. Expansion mesangial matrix,...
Recent biochemical studies involving 2′,7′-bis-(2-carboxyethyl)-5,6-carboxylfluorescein (BCECF)-labeled saponin-permeabilized and parasitized erythrocytes indicated that malaria parasite cells maintain the resting cytoplasmic pH at about 7.3, treatment with vacuolar proton-pump inhibitors reduces to 6.7, suggesting proton extrusion from via H<sup>+</sup>-ATPase (Saliba, K. J., Kirk, (1999) <i>J. Biol. Chem.</i> 274, 33213–33219). In present study, we investigated localization of in...
Abstract Bacterial DNA and synthetic oligodeoxynucleotides (ODN) containing unmethylated CpG motifs stimulate a strong innate immune response. This stimulation can be abrogated by either removing the or adding inhibitory/suppressive motifs. Suppression is dominant over specific for CpG-induced responses (having no effect on LPS- Con A-induced activation). Individual cells noncompetitively internalize both stimulatory suppressive ODN. Studies using ODN composed of indicate that sequence...
Activation of resting human CD4+ T cells mediated by mAb ligation the TCR/CD3 complex requires costimulatory signals to result in proliferation; these can be provided intercellular cell adhesion molecule-1 (ICAM-1, CD54) a natural ligand leukocyte function-associated Ag-1 (LFA-1, CD11a/CD18). We analyzed early signaling events involved activation determine contribution LFA-1/ICAM-1 interaction. studied detail hydrolysis phosphatidylinositol(4,5)bisphosphate and intracellular levels free Ca2+...
Amphiphysin 1, an endocytic adaptor concentrated at synapses that couples clathrin-mediated endocytosis to dynamin-dependent fission, was also shown have a regulatory role in actin dynamics. Here, we report amphiphysin 1 interacts with N-WASP and stimulates N-WASP- Arp2/3-dependent polymerization. Both the Src homology 3 N-BAR domains are required for this stimulation. Acidic liposome-triggered, N-WASP-dependent polymerization is strongly impaired brain cytosol of knock-out mice. FRET-FLIM...
Dynamin GTPase, a key molecule in endocytosis, mechanically severs the invaginated membrane upon GTP hydrolysis. functions also regulating actin cytoskeleton, but mechanisms are yet to be defined. Here we show that dynamin 1, neuronal isoform of dynamin, and cortactin form ring complexes, which twine around F-actin bundles stabilize them. By negative-staining EM, 1–cortactin complexes appeared as “open” or “closed” rings depending on guanine nucleotide conditions. pyrene assembly assay, 1...
Abstract In plant cells, mitochondria and plastids contain their own genomes derived from the ancestral bacteria endosymbiont. Despite limited genetic capacity, these multicopy organelle account for a substantial fraction of total cellular DNA, raising question whether DNA quantity is controlled spatially or temporally. this study, we genetically dissected decrease in pollen, phenomenon that appears to be common most angiosperm species. By staining mature pollen grains with fluorescent dye,...
The endocytic protein dynamin participates in the formation of actin-based membrane protrusions such as podosomes, pseudopodia, and invadopodia, which facilitate cancer cell migration, invasion, metastasis. However, role at leading edge cells is unclear. In this study, we demonstrate that ubiquitously expressed 2 isoform facilitates migration by stabilizing F-actin bundles filopodia lung line H1299. Pharmacological inhibition decreased filopodial formation. Furthermore, cortactin mostly...
Abstract Glioblastoma multiforme (GBM) is the most common malignant brain tumor with a median survival time about one year. Invasion of GBM cells into normal major cause poor prognosis and requires dynamic reorganization actin cytoskeleton, which includes lamellipodial protrusions, focal adhesions, stress fibers at leading edge GBM. Therefore, we hypothesized that inhibitors polymerization can suppress migration invasion. First, adopted drug repositioning system for screening...
Dynamin is a mechanochemical GTPase essential for membrane fission during clathrin-mediated endocytosis. forms helical complexes at the neck of clathrin-coated pits and their structural changes coupled with GTP hydrolysis drive fission. its binding protein amphiphysin cooperatively regulate remodeling fission, but precise mechanism remains elusive. In this study, we analyzed dynamin-amphiphysin using electron microscopy (EM) high-speed atomic force (HS-AFM). Interestingly, HS-AFM analyses...
Vacuolar H+-ATPase-dependent (V-ATPase-dependent) functions are critical for neural proteostasis and involved in neurodegeneration brain tumorigenesis. We identified a patient with fulminant of the developing carrying de novo splice site variant ATP6AP2 encoding an accessory protein V-ATPase. Functional studies induced pluripotent stem cell-derived (iPSC-derived) neurons from this revealed reduced spontaneous activity severe deficiency lysosomal acidification degradation leading to neuronal...
Membrane remodeling is required for dynamic cellular processes such as cell division, polarization, and motility. BAR domain proteins dynamins are key molecules in membrane that work together deformation fission. In striated muscles, sarcolemmal invaginations termed T-tubules excitation–contraction coupling. BIN1 DNM2, which encode a protein dynamin 2, respectively, have been reported to be causative genes of centronuclear myopathy (CNM), hereditary degenerative disease skeletal muscle,...
STUDIES ON A DI-AMINO ACID, CANAVANIN (II) Get access MATSUNOSUKE KITAGAWA, KITAGAWA Biochemical Laboratory, Department of Agriculture, Kyushu Imperial UniversityFukuoka Search for other works by this author on: Oxford Academic PubMed Google Scholar HIROSHI YAMADA The Journal Biochemistry, Volume 16, Issue 2, November 1932, Pages 339–350, https://doi.org/10.1093/oxfordjournals.jbchem.a125217 Published: 01 1932
Glomerular podocytes act as a part of filtration barrier in the kidney. The activity this filter is regulated by ionotropic and metabotropic glutamate receptors. Adjacent can potentially release into intercellular space; however, little known about how glutamate. Here, we demonstrated vesicular transporter 3 (VGLUT3)-dependent from podocytes. Immunofluorescence analysis revealed that rat glomerular an immortal mouse podocyte cell line (MPCs) express VGLUT1 VGLUT3. Consistent with finding,...