New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide oxidation the resulting magnetite sodium hypochlorite, followed addition poly(l-lysine) (PLL) solution. PLL several molecular weights ranging from 146 (l-lysine) to 579 000 was tested as a coating boost intracellular uptake nanoparticles. The characterized TEM, dynamic light scattering, FTIR, ultrasonic spectrometry. TEM revealed that particles ca. 6 nm in...

Spinal cord injury (SCI) is a condition that results in significant mortality and morbidity. Treatment of SCI utilizing stem cell transplantation represents promising therapy. However, current conventional treatments are limited by inefficient delivery strategies cells into the injured tissue. In this study, we designed magnetic system used it to accumulate labelled with superparamagnetic iron oxide nanoparticles (SPION) at specific site lesion. The loading engineered SPIONs guarantees...

New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide according to two methods. In the first method, was done in presence d-mannose solution (in situ coating); second method involved oxidation precipitated magnetite sodium hypochlorite followed addition (postsynthesis coating). Selected characterized transmission electron microscopy (TEM), atomic force (AFM), elemental analysis, dynamic light scattering, infrared...

Prodrug cancer gene therapy by mesenchymal stem cells (MSCs) targeted to tumors represents an attractive tool activate prodrugs directly within the tumor mass, thus avoiding systemic toxicity. In this study, we tested feasibility and efficacy of human adipose tissue-derived MSCs, engineered express suicide cytosine deaminase::uracil phosphoribosyltransferase treat intracranial rat C6 glioblastoma. Experiments were designed simulate conditions future clinical application for high-grade...

The transplantation of mesenchymal stem cells (MSC) is currently under study as a therapeutic approach for spinal cord injury, and the number transplanted that reach lesioned tissue one critical parameters. In this study, intrathecally labeled with superparamagnetic iron oxide nanoparticles were guided by magnetic field successfully targeted near lesion site in rat cord. Magnetic resonance imaging histological analysis revealed significant differences cell numbers distribution magnet...

Oxidative stress response in neural stem cells exposed to different superparamagnetic iron oxide nanoparticles Igor M Pongrac,1 Ivan Pavičić,2 Mirta Milić,2 Lada Brkič Ahmed,1 Michal Babič,3 Daniel Horák,3 Ivana Vinković Vrček,2 Srećko Gajović1 1School of Medicine, Croatian Institute for Brain Research, University Zagreb, 2Institute Medical Research and Occupational Health, Croatia; 3Institute Macromolecular Chemistry, Academy Sciences the Czech Republic, Prague, Republic Abstract:...

Silver (AgNPs) and maghemite, i.e., superparamagnetic iron oxide nanoparticles (SPIONs) are promising candidates for new medical applications, which implies the need strict information regarding their physicochemical characteristics behavior in a biological environment. The currently developed AgNPs SPIONs encompass myriad of sizes surface coatings, affect NPs properties may improve biocompatibility. This study is aimed to evaluate effects coating on colloidal stability modelled environments...

Maghemite (γ-Fe2O3) nanoparticles were obtained by the coprecipitation of Fe(II) and Fe (III) salts with ammonium hydroxide followed oxidation sodium hypochlorite. Solution radical polymerization N,N-dimethylacrylamide (DMAAm) in presence maghemite yielded poly(N,N-dimethylacrylamide) (PDMAAm)-coated nanoparticles. The PDMAAm on particle surface was confirmed elemental analysis ATR FTIR spectroscopy. Other methods nanoparticle characterization involved scanning transmission electron...

Experimental setup for <italic>in vitro</italic> evaluation of metallic nanoparticles where interferences depend on metal core, surface coating, and the test system.

Iron-based nanoparticles (NPs) represent small nanocarriers made of iron oxides. These include magnetite NPs (Fe3O4), maghemite (γ-Fe2O3), or mixed ferrites (metal-doped oxide NPs; MFe2O4, where M can be Co, Ni, Zn, Mn). Due to their unique characteristics, including biocompatibility, reported selective toxicity towards cancer cells, magnetic imaging capabilities, and hyperthermic properties, they are regarded as a promising modality for treatments. In this context, it is crucial assess the...

Biologic and therapeutic advances in melanoma brain metastasis are hampered by the paucity of reproducible predictive animal models. In this work, we developed a robust model that empowers quantitative tracking cellular dissemination tumor progression. Human cells labeled with superparamagnetic iron oxide nanoparticles (SPION) were injected into left cardiac ventricle mice visualized MRI. We showed SPION exposure did not affect viability, growth, or migration multiple cell lines across...

Background: Poly-L-lysine (PLL) enhances nanoparticle (NP) uptake, but the molecular mechanism remains unresolved. We asked whether PLL may interact with negatively charged glycoconjugates on cell surface and facilitate uptake of magnetic NPs (MNPs) by tumor cells. Methods: PLL-coated MNPs (PLL-MNPs) positive negative ζ-potential were prepared characterized. Confocal transmission electron microscopy was used to analyze cellular internalization MNPs. A colorimetric iron assay quantitate...

Background: Cell tracking is a powerful tool to understand cellular migration, dynamics, homing and function of stem cell transplants. Nanoparticles represent possible tracers, but they differ in uptake side effects. Their properties can be modified by coating with different biocompatible polymers. To test if polymer, poly(L-lysine), improve the biocompatibility nanoparticles applied neural cells, poly(L-lysine)-coated maghemite were prepared characterized. We evaluated their uptake,...

Iron oxide nanoparticles (IONPs) have biomedical and biotechnological applications in magnetic imaging, drug-delivery, separation purification. The biocompatibility of such particles may be improved by covering them with coating. In presented paper the biochemical anomalies liver kidney occurring animals exposed to d-mannitol-coated iron(III) (M-IONPs) were examined Fourier transform infrared (FTIR) microspectroscopy. dose IONPs used study was significantly lower than those so far other...

Abstract In this work, we present the synthesis and evaluation of magnetic resonance (MR) properties novel phosphorus/iron-containing probes for dual 31 P 1 H MR imaging spectroscopy (MRI MRS). The presented are composed biocompatible semitelechelic multivalent phospho-polymers based on poly(2-methacryloyloxyethyl phosphorylcholine) (pMPC) coordinated with small paramagnetic Fe 3+ ions or superparamagnetic maghemite ( γ -Fe 2 O 3 ) nanoparticles via deferoxamine group linked to end along...

The safety assessment of nanoparticles (NPs) is crucial during their design and development for biomedicine. One the prerequisite steps this evaluation in vitro testing that employs cell-based assays not always validated well-adapted NPs. Interferences with may arise due to nano-related optical, oxidative, fluorescent, surface catalytic properties Thus, proper validation each assay system has be performed NP type. This study aimed evaluate applicability most common cytotoxicity up-...

Magnetic resonance imaging (MRI) of superparamagnetic iron oxide-labeled cells can be used as a non-invasive technique to track stem after transplantation. The aim this study was (1) evaluate labeling efficiency D-mannose-coated maghemite nanoparticles (D-mannose(γ-Fe 2 O 3 )) in neural (NSCs) comparison the uncoated nanoparticles, (2) assess nanoparticle utilization MRI contrast agent visualize NSCs transplanted into mouse brain, and (3) test biocompatibility. D-mannose(γ-Fe ) labeled...

Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic (PLL-coated γ-Fe 2 O 3 ) studied their effect on proliferation neuronal differentiation. Materials methods: investigated the these contrast agents precursor cell...