A5045374469- Nanoparticle-Based Drug Delivery
- Selenium in Biological Systems
- Characterization and Applications of Magnetic Nanoparticles
- Analytical chemistry methods development
- Luminescence Properties of Advanced Materials
- Microfluidic and Capillary Electrophoresis Applications
- Iron oxide chemistry and applications
- Carcinogens and Genotoxicity Assessment
- Mercury impact and mitigation studies
- Carbon and Quantum Dots Applications
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Electrochemical Analysis and Applications
- Analytical Chemistry and Chromatography
- Inhalation and Respiratory Drug Delivery
- Nanoparticles: synthesis and applications
- Microfluidic and Bio-sensing Technologies
- Catalytic C–H Functionalization Methods
- Heavy Metals in Plants
- Electrospun Nanofibers in Biomedical Applications
- Fungal Plant Pathogen Control
- Heavy metals in environment
- Occupational exposure and asthma
- Forensic Entomology and Diptera Studies
- Pharmacological Receptor Mechanisms and Effects
University of Chemistry and Technology, Prague
2013-2024
Institute of Chemical Technology
2012-2014
National Institute of Public Health
1994-1996
National Institute of Public Health
1992
University of Pardubice
1985
Upconverting nanoparticles (UCNPs) are of particular interest in nanomedicine for vivo deep-tissue optical cancer bioimaging due to their efficient cellular uptake dependent on polymer coating. In this study, particles, ca. 25 nm diameter, were prepared by a high-temperature coprecipitation lanthanide chlorides. To ensure optimal dispersion UCNPs aqueous milieu, they coated with three different polymers containing reactive groups, i.e., poly(ethylene glycol)-alendronate (PEG-Ale),...
In this study, spherical or hexagonal NaYF4:Yb,Er nanoparticles (UCNPs) with sizes of 25 nm (S-UCNPs) and 120 (L-UCNPs) were synthesized by high-temperature coprecipitation subsequently modified three kinds polymers. These included poly(ethylene glycol) (PEG) poly(N,N-dimethylacrylamide-co-2-aminoethylacrylamide) [P(DMA-AEA)] terminated an alendronate anchoring group, poly(methyl vinyl ether-co-maleic acid) (PMVEMA). The internalization rat mesenchymal stem cells (rMSCs) C6 cancer (rat glial...
Single–particle analysis using inductively coupled plasma mass spectrometry offers a new tool for the characterisation of inorganic nanoparticles. Its development is connected with generations ultrafast spectrometers. This work concerned thorough investigation parameters affecting quality Ag nanoparticles, i.e., nanoparticle stability, transport efficiency and sensitivity determination. The short-term stability nanoparticles in demineralised water can be prolonged to at least 7 h by addition...
Abstract In this study, bare gold nanoparticles (GNPs) immobilized in the sol–gel‐pretreated fused‐silica (FS) capillary as a stationary phase for open‐tubular electrochromatography (OT‐CEC) are first time shown to be able separate both hydrophobic polyaromatic hydrocarbons (PAHs) well hydrophilic cationic antimicrobial peptides. Model mixture of four PAHs, naphthalene, fluorene, phenanthrene, and anthracene, was resolved by OT‐CEC GNP‐modified FS capillaries using hydro‐organic background...
Nanomaterials are currently the subject of intense research due to their wide variety potential applications in biomedical, optical and electronic fields. We prepared tested cobalt zinc ferrite nanoparticles (Co0.5Zn0.5Fe2O4+γ [CZF-NPs]) encapsulated by amorphous silica order find a safe contrast agent magnetic label for tracking transplanted cells within an organism using resonance imaging (MRI). Rat mesenchymal stem (rMSCs) were labeled 48 h with low, medium or high dose CZF-NPs (0.05;...
Introduction: Magnetic resonance (MR) imaging is suitable for noninvasive long-term tracking. We labeled human induced pluripotent stem cell-derived neural precursors (iPSC-NPs) with two types of iron-based nanoparticles, silica-coated cobalt zinc ferrite nanoparticles (CZF) and poly-l-lysine-coated iron oxide superparamagnetic (PLL-coated γ-Fe 2 O 3 ) studied their effect on proliferation neuronal differentiation. Materials methods: investigated the these contrast agents precursor cell...
Abstract A wise selection of tracers is critical for magnetic particle imaging (MPI). Most the current are based on superparamagnetic iron oxide nanoparticles (SPIONs) with a suitable coating. We prepared maghemite cores (γ-Fe 2 O 3 ) by coprecipitation Fe(II) and Fe(III) salts ammonium hydroxide followed oxidation hydrogen peroxide stabilization as an anionic ⊖ or cationic colloid ⨁ ). The were coated poly( N -(2-hydroxypropyl)methacrylamide)-co-...
Upconverting nanoparticles are attracting extensive interest as a multimodal imaging tool. In this work, we report on the synthesis and characterization of gadolinium-enriched upconverting for bimodal magnetic resonance optical luminescence imaging. NaYF4:Gd3+,Yb3+,Tm3+ core were obtained by thermal coprecipitation lanthanide oleate precursors in presence oleic acid stabilizer. With aim improving upconversion emission increasing amount Gd3+ ions nanoparticle surface, 2.5 nm NaGdF4 shell was...
Large (120 nm) hexagonal NaYF 4 :Yb, Er nanoparticles (UCNPs) were synthesized by high-temperature coprecipitation method and coated with poly(ethylene glycol)-alendronate (PEG-Ale), poly ( N , -dimethylacrylamide- co -2-aminoethylacrylamide)-alendronate (PDMA-Ale) or poly(methyl vinyl ether- -maleic acid) (PMVEMA). The colloidal stability of polymer-coated UCNPs in water, PBS DMEM medium was investigated dynamic light scattering; UCNP@PMVEMA particles showed the best PBS. Dissolution PBS,...
Abstract Manganese‐zinc ferrite nanoparticles were synthesized by using a hydrothermal treatment, coated with silica, and then tested as efficient cellular labels for cell tracking, magnetic resonance imaging (MRI) in vivo. A toxicity study was performed on rat mesenchymal stem cells C6 glioblastoma cells. Adverse effects viability proliferation observed at the highest concentration (0.55 mM) only; not compromised lower concentrations. Nanoparticle internalization confirmed transmission...
Magnetic nanoparticles of ε-Fe1.76 Ga0.24 O3 with the volume-weighted mean size 17 nm were prepared by thermal treatment a mesoporous silica template impregnated metal nitrates and coated shell four different thicknesses in range 6-24 nm. The bare particles exhibited higher magnetization than undoped compound, 22.4 Am2 kg-1 at 300 K, characterized blocked state coercivity 1.2 T being thus very opposite superparamagnetic iron oxides. relaxometric study silica-coated samples 0.47 revealed...
Speciation analysis of selenium metabolites in urine was performed using hyphenation mixed ion-pair reversed-phase chromatography and inductively coupled plasma mass spectrometry. A chromatographic separation with a C8 stationary phase mobile containing 2.5 mmol L−1 sodium butane-1-sulfonate, 8 tetramethylammonium hydroxide, 4 malonic acid, 0.05% methanol, pH 3.0. Under this condition, the species selenite, selenate, selenomethionine, selenoethionine, selenourea, trimethylselenonium,...
A method allowing the determination of total iodine content and species in samples animal tissues using inductively coupled plasma mass spectrometry (ICP-MS) as an element-specific detector was developed. The determined after microwave digestion with 25% (w/w) water solution tetramethylammonium hydroxide. detection limit 26.9 μg kg−1 I, accuracy proven through analysis SRM "Non-Fat Milk Powder," porcine liver, Atlantic Cod muscle standard addition methods. extracts for speciation were...
1. The excretion of urinary metabolites was studied in rat dosed intraperitoneally with ethyl acrylate and 1-butyl acrylate.2. Physiological carboxylic acids, namely, 3-hydroxypropanoic acid, lactic acid acetic were determined by hplc may be derived from the xenobiotic acrylates.3. A significant increase amounts excreted within 24h after dosing found both acrylate-exposed rats.4. slight (p < 0.10) also animals exposed to acrylates.5. Two mercapturic N-acetyl-S-(2-carboxyethyl)cysteine...
We prepared new phases for LC that consisted of silica modified with non-covalently bonded tetrakis(β-cyclodextrin)-porphyrin (where cyclodextrin is CD) conjugates. The effects the porphyrin core, type spacer and β-CD moieties on behaviours separation aromatic compounds (benzene, toluene, ethylbenzene, propylbenzene, butylbenzene, pentylbenzene, o-terphenyl, triphenylene, phenol caffeine) fluorinated (pentafluorobenzonitrile, pentafluoronitrobenzene hexafluorobenzene) were studied using...