A5077726494- Advanced MRI Techniques and Applications
- Nanoparticle-Based Drug Delivery
- Crystallization and Solubility Studies
- Characterization and Applications of Magnetic Nanoparticles
- X-ray Diffraction in Crystallography
- Lanthanide and Transition Metal Complexes
- Pancreatic function and diabetes
- Iron oxide chemistry and applications
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Neurogenesis and neuroplasticity mechanisms
- Advanced Neuroimaging Techniques and Applications
- Graphene and Nanomaterials Applications
- Nanoplatforms for cancer theranostics
- Advanced NMR Techniques and Applications
- RNA Interference and Gene Delivery
- Cannabis and Cannabinoid Research
- Pluripotent Stem Cells Research
- Hydrogels: synthesis, properties, applications
- MRI in cancer diagnosis
- Nerve injury and regeneration
- Tissue Engineering and Regenerative Medicine
- Anesthesia and Neurotoxicity Research
- Advanced Nanomaterials in Catalysis
- Neuroscience and Neuropharmacology Research
Charles University
2009-2024
Center for Advanced Preclinical Imaging
2021-2023
Institute of Clinical and Experimental Medicine
2011-2020
Resonance Research (United States)
2015
Czech Academy of Sciences, Institute of Experimental Medicine
2007-2014
Czech Academy of Sciences
2008-2009
Czech Academy of Sciences, Institute of Physics
2007-2009
Czech Academy of Sciences, Institute of Macromolecular Chemistry
2007-2009
National Institutes of Health
1999-2002
National Institute of Neurological Disorders and Stroke
1999-2002
Demyelination is a common pathological finding in human neurological diseases and frequently persists as result of failure endogenous repair. Transplanted oligodendrocytes their precursor cells can (re)myelinate axons, raising the possibility therapeutic intervention. The migratory capacity transplanted key importance determining extent (re)myelination can, at present, be evaluated only by using invasive irreversible procedures. We have exploited transferrin receptor an efficient...
New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide oxidation the resulting magnetite sodium hypochlorite, followed addition poly(l-lysine) (PLL) solution. PLL several molecular weights ranging from 146 (l-lysine) to 579 000 was tested as a coating boost intracellular uptake nanoparticles. The characterized TEM, dynamic light scattering, FTIR, ultrasonic spectrometry. TEM revealed that particles ca. 6 nm in...
Abstract Nuclear magnetic resonance (MR) imaging provides a noninvasive method for studying the fate of transplanted cells in vivo. We studied, animals with cortical photochemical lesion or balloon‐induced spinal cord compression lesion, implanted rat bone marrow stromal (MSCs) and mouse embryonic stem (ESCs) labeled superparamagnetic iron oxide nanoparticles (Endorem). MSCs were colabeled bromodeoxyuridine (BrdU), ESCs transfected pEGFP‐C1 (eGFP ESCs). Cells either grafted intracerebrally...
A series of high-generation (G) ethylenediamine-core polyamidoamine (PAMAM) dendrimers corresponding to G = 5, 7, 9, and 10 were conjugated with the bifunctional chelate 2-(4-isothiocyanatobenzyl)- 1,4,7,10-tetraazacyclododecane-N,N',N",N"'-tetraacetate (p-SCN-Bz-DOTA). Gadolinium (III) ion was added macromolecules, 1/T1 1/T2 NMRD profiles measured at 3 degrees, 23 37 degrees C. The synthesis resulted in preparations that ranged from an average 127 chelates 96 Gd3+ ions per 5 dendrimer 3727...
Abstract Bone marrow stromal cells (MSCs) are pluripotent progenitor that have the capacity to migrate toward lesions and induce or facilitate site‐dependent differentiation in response environmental signals. In animals with a cortical photochemical lesion, fate of rat MSCs colabeled magnetic iron‐oxide nanoparticles (Endorem®) bromodeoxyuridine (BrdU) was studied. were either grafted intracerebrally into contralateral hemisphere adult brain injected intravenously. vivo MRI used track their...
Monodisperse superparamagnetic Fe3O4 nanoparticles coated with oleic acid were prepared by thermal decomposition of Fe(III) glucuronate. The shape, size, and particle size distribution controlled varying the reaction parameters, such as temperature, concentration stabilizer, type high-boiling-point solvents. Magnetite particles characterized transmission electron microscopy (TEM), well diffraction (SAED), X-ray (XRD), dynamic light scattering (DLS), magnetometer measurements. coating was...
Magnetite (Fe3O4) nanoparticles with uniform sizes of 10, 20, and 31 nm were prepared by thermal decomposition Fe(III) oleate or mandelate in a high-boiling point solvent (>320 °C). To render the particles hydrophilic antifouling properties, their surface was coated PEG-containing bisphosphonate anchoring group. The PEGylated characterized range physicochemical methods, including dynamic light scattering, transmission electron microscopy, thermogravimetric analysis, Fourier transform...
A promising treatment method for type 1 diabetes mellitus is transplantation of pancreatic islets containing beta-cells. The aim this study was to develop an MR technique monitor the distribution and fate transplanted in animal model. Twenty-five hundred purified magnetically labeled were through portal vein into liver experimental rats. animals scanned using a 4.7-T scanner. clearly visualized both diabetic healthy rats as hypointense areas on T2*-weighted images during entire measurement...
New surface-modified iron oxide nanoparticles were developed by precipitation of Fe(II) and Fe(III) salts with ammonium hydroxide according to two methods. In the first method, was done in presence d-mannose solution (in situ coating); second method involved oxidation precipitated magnetite sodium hypochlorite followed addition (postsynthesis coating). Selected characterized transmission electron microscopy (TEM), atomic force (AFM), elemental analysis, dynamic light scattering, infrared...
In vitro labeling of pancreatic islets by iron nanoparticles enables their detection as hypoitnense spots on serial magnetic resonance (MR) images. We report the first results a pilot trial aiming to test feasibility and safety this technique in humans.Islets were labeled culture with 5 μL/mL ferucarbotran for 6 48 hr transplanted into portal vein (12 infusions) 8 C-peptide negative recipients. The liver area was examined next day 1, 4, 24 weeks posttransplant using 3T MR scanner.In all...
The simultaneous combination of optical and magnetic resonance imaging (MRI) would greatly benefit in vivo disease diagnosis as well situ monitoring living cells. In order to design dual detection cells involving by fluorescent microscopy MRI, nanoparticles with two reporters, a dye superparamagnetic core, included one particle were synthesized characterized. γ-Fe2O3 obtained coprecipitation oxidation coated silica (SiO2) or carboxymethyl chitosan (CMCS) labeled fluorescein isothiocyanate...
The transplantation of mesenchymal stem cells (MSC) is currently under study as a therapeutic approach for spinal cord injury, and the number transplanted that reach lesioned tissue one critical parameters. In this study, intrathecally labeled with superparamagnetic iron oxide nanoparticles were guided by magnetic field successfully targeted near lesion site in rat cord. Magnetic resonance imaging histological analysis revealed significant differences cell numbers distribution magnet...
Maghemite (γ-Fe2O3) nanoparticles were obtained by the coprecipitation of Fe(II) and Fe (III) salts with ammonium hydroxide followed oxidation sodium hypochlorite. Solution radical polymerization N,N-dimethylacrylamide (DMAAm) in presence maghemite yielded poly(N,N-dimethylacrylamide) (PDMAAm)-coated nanoparticles. The PDMAAm on particle surface was confirmed elemental analysis ATR FTIR spectroscopy. Other methods nanoparticle characterization involved scanning transmission electron...
Abstract Introduction A growing number of studies have highlighted the potential stem cell and more-differentiated neural transplantation as intriguing therapeutic approaches for repair after spinal cord injury (SCI). Methods conditionally immortalized line derived from human fetal tissue (SPC-01) was used to treat a balloon-induced SCI. SPC-01 cells were implanted into lesion 1 week To determine feasibility tracking transplanted cells, portion labeled with poly-L-lysine-coated...
Upconverting nanoparticles (UCNPs) are of particular interest in nanomedicine for vivo deep-tissue optical cancer bioimaging due to their efficient cellular uptake dependent on polymer coating. In this study, particles, ca. 25 nm diameter, were prepared by a high-temperature coprecipitation lanthanide chlorides. To ensure optimal dispersion UCNPs aqueous milieu, they coated with three different polymers containing reactive groups, i.e., poly(ethylene glycol)-alendronate (PEG-Ale),...
Abstract A novel bimodal fluorescence/MRI probe based on a cyclodextrin scaffold has been synthesized and characterized. The final agent employs the fluorescein (F) functionality as fluorescence marker Gd III complex of macrocyclic DOTA‐based ligand (GdL) having one aminobenzyl‐phosphinic acid pendant arm an MRI probe, statistical composition 6.9 ‐F 0.1 ‐β‐CD. Slow rotational dynamics (governed by very rigid scaffold) combined with fast water exchange (ensured chosen ligand) resulted in high...
Multimodal imaging-therapeutic nanoprobe TiO(2)@RhdGd was prepared and successfully used for in vitro vivo cell tracking as well killing of cancer cells vitro. TiO(2) nanoparticles were a core phosphonic acid modified functionalities, responsible contrast MRI optical imaging. The probe shows high (1)H relaxivity density values. Presence fluorescent dye allows visualization by means fluorescence microscopy. applicability the studied, using mesenchymal stem cells, HeLa T-lymphocytes. did not...
1,8-Bis(2,2,2-trifluoroethyl)cyclam (te2f) derivatives with two coordinating pendant arms involving methylenecarboxylic acid (H2te2f2a), methylenephosphonic (H4te2f2p), (2-pyridyl)methyl (te2f2py), and 2-aminoethyl (te2f2ae) in 4,11-positions were prepared, their nickel(II) complexes investigated as possible 19F MR tracers. The solid-state structures of several synthetic intermediates, ligands, all confirmed by X-ray diffraction analysis. average Ni···F distances determined to be about 5.2...
In this study, spherical or hexagonal NaYF4:Yb,Er nanoparticles (UCNPs) with sizes of 25 nm (S-UCNPs) and 120 (L-UCNPs) were synthesized by high-temperature coprecipitation subsequently modified three kinds polymers. These included poly(ethylene glycol) (PEG) poly(N,N-dimethylacrylamide-co-2-aminoethylacrylamide) [P(DMA-AEA)] terminated an alendronate anchoring group, poly(methyl vinyl ether-co-maleic acid) (PMVEMA). The internalization rat mesenchymal stem cells (rMSCs) C6 cancer (rat glial...
Core-shell nanoparticles consisting of La(0.75)Sr(0.25)MnO(3) cores covered by silica were synthesized a procedure several steps, including the sol-gel method in presence citric acid and ethylene glycol, thermal mechanical treatment, encapsulation employing tetraethoxysilane final separation centrifugation order to get required size fraction. Morphological studies revealed well-separated particles that form stable water suspension. Magnetic include magnetization measurements investigation...
Combining specific and quantitative F-19 magnetic resonance imaging (MRI) with sensitive convenient optical provides complementary information about the distribution viability of transplanted pancreatic islet grafts. In this study, islets (PIs) were labeled positively charged multimodal nanoparticles based on poly(lactic-co-glycolic acid) (PLGA-NPs) encapsulated perfluoro-15-crown-5-ether near-infrared fluorescent dye indocyanine green.One thousand three bioluminescent PIs into subcutaneous...
Background In Wilson's disease (WD), demyelination, rarefaction, gliosis, and iron accumulation in the deep gray matter cause opposing effects on T 2 ‐weighted MR signal. However, degree interplay of these changes chronically treated WD patients has not been quantitatively studied. Purpose To compare differences brain multiparametric mapping between controls with neurological (neuro‐WD) hepatic (hep‐WD) forms to infer nature residual neuropathology. Study Type Cross‐sectional....