Receptor for advanced glycation end products (RAGE) may be involved in the pathogenesis of cancer progression and metastasis. Pathological effects mediated via RAGE are physiologically inhibited by soluble (sRAGE), so higher sRAGE levels confer patients with better outcome. The aim was to study gene polymorphisms breast cancer. authors studied 120 (subdivided based on clinical stage, histologic grading, expression hormonal Her2/neu receptors) 92 healthy controls. Despite serum concentrations...

The receptor for advanced glycation end products (RAGE) is involved in the pathogenesis of vascular and inflammatory diseases. pathological effects mediated via RAGE are physiologically inhibited by soluble (sRAGE). Our aim was to study sRAGE gene polymorphisms haemodialysis (HD) patients.A total 261 stable HD patients were enrolled prospectively followed up 30 months. At begining study, nutritional parameters determined. determined a subgroup 214 patients. A group 100 healthy controls used...

Background:Obstructive sleep apnea (OSA) is a condition contributing to oxidative stress. The aim of this study was ascertain if there any connection between OSA and novel stress-related markers. Matrix metalloproteinases 2 9 (MMP-2, MMP-9), high sensitive C-reactive protein (hsCRP), pregnancy-associated plasma protein-A (PAPP-A), soluble receptors for advanced glycation end-products (sRAGE), zinc (Zn) copper (Cu) were measured. Further biochemical markers...

Biocompatible DNA origami nanostructures (DONs) attract significant interest as potential targeted drug carriers. Their sensitivity to various environmental triggers is beneficial a release or decomposition mechanism, but it also hinders their stability; therefore, they require some extent of cross-linking. Using cisplatin, we demonstrate that molecule can both act therapeutic and cross-linking agent for DONs. In this work, triangular two-dimensional DONs are loaded with ∼1000 cisplatin...

Alterations in blood and tissue concentrations of trace elements patients with chronic renal failure have been extensively investigated. Selenium, zinc copper are which play an important role biological systems as components proteins, enzymes antioxidants. The selenium, were determined the plasma, erythrocytes whole on regular hemodialysis (HD) continuous ambulatory peritoneal dialysis (CAPD) treatment using method inductively coupled plasma mass spectrometry (ICP-MS). Analysis isotopes...

Single–particle analysis using inductively coupled plasma mass spectrometry offers a new tool for the characterisation of inorganic nanoparticles. Its development is connected with generations ultrafast spectrometers. This work concerned thorough investigation parameters affecting quality Ag nanoparticles, i.e., nanoparticle stability, transport efficiency and sensitivity determination. The short-term stability nanoparticles in demineralised water can be prolonged to at least 7 h by addition...

A model small-scale field experiment was set up to investigate selenium (Se) uptake by four different varieties of broccoli plants, as well the effect Se foliar application on essential elements for plants calcium (Ca), copper (Cu), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn), phosphorus (P), sulfur (S), and zinc (Zn). Foliar sodium selenate (Na2SeO4) carried out at two rates (25 50 g Se/ha), an untreated control variant included. Analyses individual parts were performed,...

Introduction. Circulating tumor cells (CTCs) detection prior to and during therapy is considered as an independent strong prognostic marker. The present study was designed isolate characterize CTCs in peripheral blood of early breast cancer (BC) patient a biomarker for monitoring treatments efficacy. Materials methods. In total, 54 patients undergoing neoadjuvant and/or adjuvant chemotherapy regimens were enrolled into prospective study. CTC performed by AdnaTest BreastCancer™ (AdnaGen AG,...

Advanced glycation end products (AGEs) take part in the pathogenesis of vascular, diabetic, and uremic complications. Their precursors are detoxified by glyoxalase system. Our aim was to study A419C (E111A) single nucleotide polymorphism (SNP) I gene hemodialysis (HD) patients. SNP, several laboratory parameters including soluble receptor for AGEs (sRAGE), clinical data were studied 214 HD patients 89 controls. Allelic genotypic frequencies did not differ between SNP significantly linked...

Abstract Chromium (Cr), an essential element, mainly affects saccharide (potentiated insulin action via interaction with receptor on the cell surface) and lipid metabolism (inhibition of hydroxymethylglutaryl‐CoA reductase a hypolipidemic effect). The aim study was to describe Cr serum levels in different diseases (malignant, metabolic, renal) using advanced analytical technique correlation other biochemical parameters. concentration measured atomic absorption spectrometry electrothermal...

Reversed-phase liquid chromatography hyphenated with inductively coupled plasma mass spectrometry (LC-ICP-MS) was used for mercury speciation analysis in food samples. A short chromatographic column (Purospher® RP-8e, 75 ´ 4 mm, 3 µm) and a mobile phase containing 0.02 mol/l CH3COONH4 + 0.2% (v/v) 2-mercaptoethanol (2-ME) 1% CH3OH were applied. repeated extraction of samples hydrochloric acid/2-ME solution (1 HCl 2-ME) applied as the isolation step. The results satisfactory most matrices...

A method was developed for the determination of selenium in human blood by ICP-MS. The procedure involves sample dilution with Triton X-100 and HNO 3 (1 ml whole blood+1 1% X-100+1.5 0.14 mol l -1 +0.2 5 mg In solution+water to 10 ml) using 77 Se line. Standard samples were modified a solution containing NaCl, Fe, Ca, Br, S, K. No corrections made interferences from polyatomic ions. applicability ICP-MS, ICP-OES, ETAAS HGAAS techniques compared. Limits detection (3 s ) these methods 6, 76,...