- Potato Plant Research
- Plant Pathogens and Resistance
- Dialysis and Renal Disease Management
- Agriculture, Plant Science, Crop Management
- Phytochemicals and Antioxidant Activities
- Microbial Metabolites in Food Biotechnology
- Protein Hydrolysis and Bioactive Peptides
- Antioxidant Activity and Oxidative Stress
- Plant-Microbe Interactions and Immunity
- Selenium in Biological Systems
- Education, Psychology, and Social Research
- Nitric Oxide and Endothelin Effects
- Vitamin C and Antioxidants Research
- Bee Products Chemical Analysis
- Phytoestrogen effects and research
- Phytase and its Applications
- Food Chemistry and Fat Analysis
- Silymarin and Mushroom Poisoning
- Blood Pressure and Hypertension Studies
- Enzyme Production and Characterization
- Transgenic Plants and Applications
- Botany and Plant Ecology Studies
- Image Processing and 3D Reconstruction
- Glutathione Transferases and Polymorphisms
- Sesame and Sesamin Research
University of South Bohemia in České Budějovice
2011-2024
Slovak University of Agriculture in Nitra
2023
Stellenbosch University
2023
Ministry of Agriculture
2023
Federation of the Food and Drink Industries of the Czech Republic
2023
Agroécologie
2009
Sewanee: The University of the South
2008
Charles University
1993-1998
Cardialysis (Netherlands)
1998
Oilseed cakes are produced as a by-product of oil pressing and mostly used feed. Their use for human consumption is due to the functional properties benefits health. Herein, oilseed cake flours eight species (flax, hemp, milk thistle, poppy, pumpkin, rapeseed, safflower, sunflower) were sieved into fractions above (A250) below (B250) 250 µm. The chemical composition, SDS-PAGE profiles, colour, antioxidant activities these evaluated. B250 evaluated being protein ash rich, reaching crude...
Effects of protein precipitators, ethanol and ferric chloride, on yield, resolubility, chemical composition nutritional value concentrates isolated from industrial potato fruit juice (PFJ) were studied. Optimum precipitating concentrations chloride in PFJ 4 M (23.1% v/v) 20 mM (2% w/v), resulting yield 69% 86.5% total protein, respectively. Contents glycoalkaloids potassium both significantly lower (P < 0.05) as compared with contents dry matter. Both exhibited high value; values essential...
Biochemical characteristics of patatin proteins purified by ion-exchange and affinity chromatography from tubers 20 potato cultivars were studied to evaluate their genotype differences with respect utility groups, table (TPCs) processing (PPCs). Both groups showed similar values protein content in dry matter (3.98-7.39%) relative abundance (5.40-35.40%). Three mass levels (∼40.6, 41.8, 42.9 kDa) patatins found MALDI-TOF MS within all cultivars. Differences among corresponding the sugar...
Patatin relative abundance in SDS-extractable protein and patatin content dry matter were evaluated tubers of forty processing table potato cultivars usually cultivated the Czech Republic, Germany, Netherlands. The characteristics over three experimental years. achieved on average a significantly higher value (P < 0.001; Tukey HSD test) than cultivars, resulting values 25.80% 21.59%, respectively. A high (over 30% extractable protein) was determined only case two cultivars: Vaneda (average...
Alterations in blood and tissue concentrations of trace elements patients with chronic renal failure have been extensively investigated. Selenium, zinc copper are which play an important role biological systems as components proteins, enzymes antioxidants. The selenium, were determined the plasma, erythrocytes whole on regular hemodialysis (HD) continuous ambulatory peritoneal dialysis (CAPD) treatment using method inductively coupled plasma mass spectrometry (ICP-MS). Analysis isotopes...
It is hypothesized that oligosaccharides are another potential source of immunological cross-reaction between different plant allergens. Patatin the most abundant glycoprotein in potato and has been described to have an oligosaccharide composition Man3(Xyl)GlcNAc2(Fuc). In this work, N-glycosylation profiles patatin proteins isolated from tubers species were investigated compared. Oligosaccharides released by enzymatic digestion with PNAGase A analyzed primarily matrix-assisted laser...
Faba beans (Vicia faba L.) are a high-protein legume crop that can be widely cultivated in most climates Europe. The amino acid composition of the bean protein is also beneficial for monogastric animals since it contains great deal lysine, an deficient cereals. Two genotypes were at three sowing densities (45, 60 and 75 seeds per m2) during growing seasons (years 2013–2015). aim research was to assess accumulation nutrients (N, P, K, Ca Mg) two straw under different densities. A field...
Onion skin is a waste produced in thousands of tons annually the European Union. It rich natural source flavonoids and its water extracts (as an environmentally friendly solvent) could be used as antioxidant material for meat products. Therefore, properties onion (OSWEs) were tested on cooked pork patties. Pork patties divided into five treatments: control (no antioxidant), 10 20% (w/w) yellow OSWE, red OSWE. Antioxidant activity, total polyphenols, thiobarbituric acid reactive substances...
The biological effect of oxygen-reactive species controlled by antioxidant mechanisms are exerted on the basis enzymes and substrates. In this study, activities enzymes-superoxide dismutase (SOD) glutathione peroxidase (GPx)-were determined in erythrocytes patients regular haemodialysis treatment. SOD activity was significantly lower (1,810.38 +/- 609.85 vs. 2,347.13 502.51 U/g haemoglobin, p < 0.05, or 70.71 11.50 100.13 24.28 mU/10(6) erythrocytes, 0.0001), as GPx (18.80 4.22 23.26 3.61...
The research is focused on the quantitative evaluation of flaxseed (Linum usitatissimum L.) proteome at level seed cake (SC), fine flour—sieved a fraction below 250 µm (FF)—and protein concentrate (PC). was performed three oilseed flax cultivars (Agriol, Raciol, and Libra) with different levels α-linolenic acid content using LC-MS/MS (shotgun proteomics) analysis, which finalized by database searching NCBI for Linum related species. A total 2560 groups (PGs) were identified, their relative...
Secondary amyloidosis is a complication typical for patients on long-term hemodialysis. The first clinical signs are usually shoulder joint pain and carpal tunnel syndrome (CTS). We have questioned 74 who were regular hemodialysis (HD) treatment divided them into 3 groups according to the length of HD. Group 1—on HD 1–4 years: There 35 in this group, 15 (i.e., 43%) had andlor CTS. None these symptoms that would require surgical treatment. II—on HDfor 5–9 22 68%) and/or Three from group...
As a source of nutritionally important components, hemp seeds are often dehulled for consumption and food applications by removing the hard hulls, which increases their nutritional value. The hulls thus become waste, although they may contain valuable protein items, about there is lack information. present work therefore aimed at evaluating proteome (Cannabis sativa L.) whole-seed, seed, hull levels. evaluation was performed on two cultivars, Santhica 27 Uso-31, using LC-MS/MS analysis. In...
The contribution deals with thermal stability and re-solubility of potato tuber proteins isolated by ethanol precipitation from industrial fruit juice. protein isolate was exposed to the temperatures ranging 25°C 70°C. Patatin, a high nutritious value, detected SDS-PAGE in region 39-43 kDa. Patatin evaluated as sensitive - above 30°C caused its strong insolubility. Potato protease inhibitors were 4.3 24 Thermal (region 25 14 kDa) higher, although 45°C denaturation insolubility most...
1. Bach, V., Mikkelsen, L., Kidmose, U., Edelenbos, M. (2015). Culinary preparation of beetroot (Beta vulgaris L.): The impact on sensory quality and appropriateness. Journal the Science Food Agriculture, 95, 1852-1859. CrossRef Google Scholar
The influence of pumpkin seed roasting conditions (110-140 °C) and screw-pressing on the formation previously undescribed Δ7-phytosterol oxidation products tocopherylquinone adducts with nucleophilic phosphatidylethanolamine species was investigated. process paste at a temperature above 120 °C for 30 min considerably enhanced Δ7-oxysterols. Targeted analysis [electron impact mass spectrometry (MS), 1D-nuclear magnetic resonance] led to identification five novel markers roasting, among which...
The utilization of plant by-products as functional food ingredients has received increasing attention in the last decade. One such by-product generated during milk thistle oil pressing is oilseed cakes, which could be used a novel ingredient. Therefore, study aimed at investigating effects addition cake (MTOC) flour fractions obtained via dry sieving, differing particle size (unsieved; coarse: >710 µm; medium: 315−710 and fine: <315 µm), on quality gluten-free bread stability silymarin...
The biological effects of reactive oxygen species and other radicals controlled by antioxidant mechanisms are modified various enzymes substrates. Antioxidant substrates divided into those with lipophilic hydrophilic groups. Retinol tocopherol the main representations antioxidants. aim present study was to describe changes retinol α-tocopherol which occurred in hemodialysis (HD) patients respect influence systems. experimental group consisted 14 on regular HD treatment. control healthy blood...
Abstract Seasonal dynamics of total soil nitrogen, ammonium and nitrate ions contents were evaluated for a processing potato field experiment conducted on three sites with different altitudes. Fertilization levels 100 200 kg N ha−1 applied before crop planting compared. Content ranged from 1.5 to 149 mg kg−1 3.0 108 dry matter basis, respectively showed strong seasonal rapid increase the content during June subsequent fall down initial values. The nitrogen fertilization level had minimal...