We isolated several strains of human and animal origin, focusing on the genera Lactobacillus Bifidobacterium. Samples cow colostrum, feces saliva calves piglets, infant were acquired, bacterial identified, twenty lactobacilli bifidobacteria shortlisted for further in vitro analysis adhesion capacity to cells, surface hydrophobicity, auto-aggregation. Adhesion was evaluated using a mixture Caco-2 HT-29-MTX cells hydrophobicity measured microbial hydrocarbon method. reuteri most frequently...

Yeasts have the remarkable capability to transform and integrate inorganic selenium into their cellular structures, thereby enhancing its bioavailability reducing toxicity. In recent years, yeasts attracted attention as potential alternative sources of protein. This study explores accumulation two less explored yeast strains, namely probiotic Saccharomyces boulardii CCDM 2020 Pichia fermentas 2012, in comparison extensively studied cerevisiae 272. Our investigation encompassed diverse stress...

Pfaffia glomerata (Amaranthaceae) is popularly known as "Brazilian ginseng." Previous studies have shown that fructose the major carbohydrate component present in its roots. Inulin-type fructans, polymers of fructose, are most widespread and researched prebiotics. Here, we isolated chemically characterized inulin extracted from P. roots investigated potential prebiotic effect. Fructans were their structures determined using colorimetric, chromatography, polarimetry, spectroscopic analysis....

Stevia rebaudiana (Bertoni) is widely studied because of its foliar steviol glycosides. Fructan-type polysaccharides were recently isolated from roots. Fructans are reserve carbohydrates that have important positive health effects and technological applications in the food industry. The objective present study was to isolate characterize fructo-oligosaccharides (FOSs) S. roots vitro adventitious root cultures evaluate potential prebiotic effect these molecules. obtained using a roller bottle...

Selenized lactic acid bacteria (LAB) represent potentially safe and effective sources of selenium (Se), essential for human health, as fermentation improves Se bioavailability reduces its toxicity. LAB are generally recognized (GRAS) widely used in fermented dairy products. To facilitate selenized implementation a functional food, we developed characterized new Se-enriched strains based on the food industry commercial Streptococcus thermophilus CCDM 144 Enterococcus faecium 922A...

The unicellular green microalga Dunaliella is a potential source of wide range nutritionally important compounds applicable to the food industry. aim this study was assess effect salina dried biomass on growth and adherence 10 strains Lactobacillus, Lacticaseibacillus, Bifidobacterium. immunomodulatory, antioxidant, cytotoxic effects D. human peripheral mononuclear cells simulated intestinal epithelial cell lines Caco-2 HT-29 were evaluated. Furthermore, hypocholesterolemic microalgae lipid...

The selenium (Se) enrichment of yeasts and lactic acid bacteria (LAB) has recently emerged as a novel concept; the individual health effects these beneficial microorganisms are combined by supplying essential micronutrient Se in more bioavailable less toxic form. This study investigated bioavailability strains Enterococcus faecium CCDM 922A (EF) Streptococcus thermophilus 144 (ST) their respective Se-enriched forms, SeEF SeST, CD (SD-Sprague Dawley) IGS rat model. LAB administration resulted...

In the present study, we evaluated ability of inorganic Se to accumulate and biotransform into different selenium species nanoparticles (SeNPs) in seven bacteria from Lactococcus, Streptococcus, Enterococcus genera. The cytotoxicity selenized strains human adenocarcinoma Caco-2 HT29 cells, effect enrichment on antioxidant activity, cell surface hydrophobicity tested were determined. When compared that lactococci enterococci, increasing concentrations sodium selenite cultivation media...

B. animalis ssp.animalis original culture MRS broth / agar 6.2 + L

The market for new functional foods and food supplements is rapidly evolving, with a current emphasis on using natural sources. Algae, probiotics, colostrum are rich sources of nutrients bioactive compounds positive effects human animal health. To determine the potential developing combining these components, we evaluated their synergistic effects. We assessed growth selected bifidobacteria in medium supplemented Chlorella vulgaris its immunomodulatory cytotoxic peripheral mononuclear cells...

The microalga Chlorella and strains of Bifidobacterium have been used in human or animal food supplements for decades because their positive health effects. presented study assessed different properties C. vulgaris its combination with bifidobacteria the aim to develop new functional foods. growth four milk whey supplemented 1.0% (w/v) immunomodulatory effects aqueous solutions (0.5%, 1.0%, 3.0%) on peripheral mononuclear cells were evaluated. Furthermore, synergistic lipid metabolism rats...

Over the past decade, use of bovine colostrum and its bioactive components as basis functional food dietary supplements for humans has substantially increased. However, developing new products enriched with probiotics colostrum, influence composition on growth promotion bacteria still needs to be tested. Therefore, we decided study chemical mineral well content compounds (immunoglobulins, lactoferrin, lactoperoxidase) ten selected strains from genera Lactobacillus, Lacticaseibacillus,...

The ability of bacteria to adhere the intestinal mucosa is a critical property necessary for long-term colonization tract. This can be highly sensitive presence prebiotics. However, limited data are available in this respect beneficial such as probiotics or resident gut microbiota. We previously demonstrated that prebiotics may decrease adherence several pre- and probiotic combinations. Thus, characterizing interactions between numerous combinations involving different classes crucial...

Infant feces and breast milk are sources of lactic acid bacteria with interesting functional technological properties. Therefore, we focused on the isolation characterization new potential probiotic strains from infant milk. Nine were identified based 16S rRNA sequences Lacticaseibacillus rhamnosus, Limosilactobacillus reuteri, Lactobacillus gasseri. Subsequently, these isolates evaluated for their safety (antibiotic resistance, hemolytic, enzymatic activity) properties (gastrointestinal...

The aim of this study was to characterize the adherence, immunomodulatory and other health properties safety Enterococcus faecium CCDM 922 from Culture Collection Dairy Microorganisms Laktoflora®. E. is susceptible therapeutically important antibiotics exhibits good adherence Caco-2 cells (52.33 ± 10.11 %). Two different concentrations were used for three five-day stimulation human mononuclear obtained peripheral blood. Levels IFN-γ, IL-6, IL-10, IL-17 IL-1α dependent on density time...

The ability to adhere intestinal epithelia is a crucial probiotic property since the retention of bacteria in large intestine important for function. Previously, we have shown that presence prebiotics decreases adherence several prebiotic- combinations. objective this study was test effect three commercially available prebiotics: Orafti GR, P95, and Synergy on five Lactobacillus strains (Lactobacillus delbrueckii subsp. bulgaricus CCDM 66, casei paracasei PE1TB-P, fermentum RL25, animalis...

<br><i>Cereus hildmannianus </i>is a species with various nutritional and medicinal properties; however, the fruits flowers have scarcely been explored. In this regard, study investigated bioproduction of total sugar content (TSC), phenolic (TPC), flavonoid (TFC), antioxidant [DPPH – 2,2-diphenyl-1-picrylhydrazyl ABTS 2,2'-azino-bis-(-3-ethylbenzothiazoline-6-sulfonic acid)], iron chelation, prebiotic activities methanolic extracts from (epicarps EE, mesocarps ME) (sepals SE, petals PE)...