- Selenium in Biological Systems
- Trace Elements in Health
- Analytical chemistry methods development
- Heavy metals in environment
- Mercury impact and mitigation studies
- Mass Spectrometry Techniques and Applications
- Arsenic contamination and mitigation
- Radiopharmaceutical Chemistry and Applications
- Moringa oleifera research and applications
- Electrochemical Analysis and Applications
- Heavy Metal Exposure and Toxicity
- Mycorrhizal Fungi and Plant Interactions
- Algal biology and biofuel production
- Nanoparticles: synthesis and applications
- Fungal Biology and Applications
- Lichen and fungal ecology
- Nonlinear Optical Materials Studies
- Laser-Ablation Synthesis of Nanoparticles
- Analytical Chemistry and Chromatography
- Heavy Metals in Plants
- Gold and Silver Nanoparticles Synthesis and Applications
- Microbial Metabolites in Food Biotechnology
- Lanthanide and Transition Metal Complexes
- Coal and Its By-products
- Plant Micronutrient Interactions and Effects
University of Chemistry and Technology, Prague
2016-2025
Institute of Chemical Technology
2011-2012
Yeasts have the remarkable capability to transform and integrate inorganic selenium into their cellular structures, thereby enhancing its bioavailability reducing toxicity. In recent years, yeasts attracted attention as potential alternative sources of protein. This study explores accumulation two less explored yeast strains, namely probiotic Saccharomyces boulardii CCDM 2020 Pichia fermentas 2012, in comparison extensively studied cerevisiae 272. Our investigation encompassed diverse stress...
Pulsed laser ablation in liquids is one of the most versatile and widespread techniques for easy synthesis different types nanoparticles with controllable properties. A huge amount energy compressed into pulse that directed onto a solid target leads to ejection materials surrounding liquid. However, precision focus irradiation can play crucial role nanomaterials and, hence, significantly affect their physico-chemical In this paper, we investigated influence position spot on optical...
Selenized lactic acid bacteria (LAB) represent potentially safe and effective sources of selenium (Se), essential for human health, as fermentation improves Se bioavailability reduces its toxicity. LAB are generally recognized (GRAS) widely used in fermented dairy products. To facilitate selenized implementation a functional food, we developed characterized new Se-enriched strains based on the food industry commercial Streptococcus thermophilus CCDM 144 Enterococcus faecium 922A...
The unicellular green microalga Dunaliella is a potential source of wide range nutritionally important compounds applicable to the food industry. aim this study was assess effect salina dried biomass on growth and adherence 10 strains Lactobacillus, Lacticaseibacillus, Bifidobacterium. immunomodulatory, antioxidant, cytotoxic effects D. human peripheral mononuclear cells simulated intestinal epithelial cell lines Caco-2 HT-29 were evaluated. Furthermore, hypocholesterolemic microalgae lipid...
The selenium (Se) enrichment of yeasts and lactic acid bacteria (LAB) has recently emerged as a novel concept; the individual health effects these beneficial microorganisms are combined by supplying essential micronutrient Se in more bioavailable less toxic form. This study investigated bioavailability strains Enterococcus faecium CCDM 922A (EF) Streptococcus thermophilus 144 (ST) their respective Se-enriched forms, SeEF SeST, CD (SD-Sprague Dawley) IGS rat model. LAB administration resulted...
In the present study, we evaluated ability of inorganic Se to accumulate and biotransform into different selenium species nanoparticles (SeNPs) in seven bacteria from Lactococcus, Streptococcus, Enterococcus genera. The cytotoxicity selenized strains human adenocarcinoma Caco-2 HT29 cells, effect enrichment on antioxidant activity, cell surface hydrophobicity tested were determined. When compared that lactococci enterococci, increasing concentrations sodium selenite cultivation media...
Single–particle analysis using inductively coupled plasma mass spectrometry offers a new tool for the characterisation of inorganic nanoparticles. Its development is connected with generations ultrafast spectrometers. This work concerned thorough investigation parameters affecting quality Ag nanoparticles, i.e., nanoparticle stability, transport efficiency and sensitivity determination. The short-term stability nanoparticles in demineralised water can be prolonged to at least 7 h by addition...
A model small-scale field experiment was set up to investigate selenium (Se) uptake by four different varieties of broccoli plants, as well the effect Se foliar application on essential elements for plants calcium (Ca), copper (Cu), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn), phosphorus (P), sulfur (S), and zinc (Zn). Foliar sodium selenate (Na2SeO4) carried out at two rates (25 50 g Se/ha), an untreated control variant included. Analyses individual parts were performed,...
Ultrafast laser processing possesses unique outlooks for the synthesis of novel nanoarchitectures and their further applications in field life science. It allows not only formation multi-element nanostructures with tuneable performance but also provides various non-invasive laser-stimulated modalities. In this work, we employed ultrafast manufacturing silicon–gold nanocomposites (Si/Au NCs) Au mass fraction variable from 15% (0.5 min ablation time) to 79% (10 min) which increased plasmonic...
The market for new functional foods and food supplements is rapidly evolving, with a current emphasis on using natural sources. Algae, probiotics, colostrum are rich sources of nutrients bioactive compounds positive effects human animal health. To determine the potential developing combining these components, we evaluated their synergistic effects. We assessed growth selected bifidobacteria in medium supplemented Chlorella vulgaris its immunomodulatory cytotoxic peripheral mononuclear cells...
Pollution and poisoning with carcinogenic arsenic (As) is of major concern globally. Interestingly, there are ferns that can naturally tolerate remarkably high As concentrations in soils while hyperaccumulating this metalloid their fronds. Besides Pteris vittata which As-related traits molecular determinants have been studied detail, the hyperaccumulation status has attributed also to cretica. We thus inspected two P. cretica cultivars, Parkerii Albo-lineata, for traits. The cultivars were...
The microalga Chlorella and strains of Bifidobacterium have been used in human or animal food supplements for decades because their positive health effects. presented study assessed different properties C. vulgaris its combination with bifidobacteria the aim to develop new functional foods. growth four milk whey supplemented 1.0% (w/v) immunomodulatory effects aqueous solutions (0.5%, 1.0%, 3.0%) on peripheral mononuclear cells were evaluated. Furthermore, synergistic lipid metabolism rats...
The combination of photodynamic therapy and radiotherapy has given rise to a modality called radiodynamic (RDT), based on reactive oxygen species-producing radiosensitizers. production singlet oxygen, O2(1Δg), by octahedral molybdenum (Mo6) clusters upon X-ray irradiation allows for simplification the architecture radiosensitizing systems. In this context, we prepared system using copper-free click chemistry between Mo6 cluster bearing azido ligands homo-bifunctional linker bis-dPEG11-DBCO....
Abstract Coordination compounds of lanthanides are indispensable in biomedical applications as MRI contrast agents and radiotherapeutics. However, since the introduction chelator DOTA four decades ago, there has been only limited progress on improving their thermodynamic stability kinetic inertness, which essential for safe vivo use. Here, we present ClickZip, an innovative synthetic strategy employing a coordination-templated formation 1 ,5 -triazole bridge that improves inertness up to...
Over the past decade, use of bovine colostrum and its bioactive components as basis functional food dietary supplements for humans has substantially increased. However, developing new products enriched with probiotics colostrum, influence composition on growth promotion bacteria still needs to be tested. Therefore, we decided study chemical mineral well content compounds (immunoglobulins, lactoferrin, lactoperoxidase) ten selected strains from genera Lactobacillus, Lacticaseibacillus,...