A5063648740- Plant Water Relations and Carbon Dynamics
- Plant responses to elevated CO2
- Plant Surface Properties and Treatments
- Photosynthetic Processes and Mechanisms
- Tree-ring climate responses
- Mass Spectrometry Techniques and Applications
- Botany and Plant Ecology Studies
- Heavy Metals in Plants
- Heavy metals in environment
- Atmospheric chemistry and aerosols
- Plant and animal studies
- Plant Parasitism and Resistance
- Plant and Biological Electrophysiology Studies
- Plant Stress Responses and Tolerance
- Mycorrhizal Fungi and Plant Interactions
- Chromium effects and bioremediation
- Plant tissue culture and regeneration
- Microbial Community Ecology and Physiology
- Aluminum toxicity and tolerance in plants and animals
- Plant Molecular Biology Research
- Advanced Proteomics Techniques and Applications
- Biocrusts and Microbial Ecology
- Wheat and Barley Genetics and Pathology
- Horticultural and Viticultural Research
- Mycotoxins in Agriculture and Food
University of South Bohemia in České Budějovice
2014-2024
University of Chemistry and Technology, Prague
2012-2024
Czech Academy of Sciences, Biology Centre
2008-2017
Institute of Plant Molecular Biology
2004-2017
Czech Academy of Sciences
1991-2015
Institute of Chemical Technology
2006-2014
In-Q-Tel
2014
Sewanee: The University of the South
2001-2011
Nemocnice České Budějovice
1995-2007
University of Toronto
1995
Mesophyll conductance (gm) and stomatal (gs) are two crucial components of the diffusive limitation photosynthesis. Variation gm in response to CO2 concentration was evaluated by using independent methods based on measurements variable electron transport rate (J) instantaneous carbon isotope discrimination, respectively. Both estimation showed a very similar shape gm/Ci relationship, with an initial increase at low substomatal concentrations (Ci), peak 180–200 μmol mol−1Ci, subsequent...
Plants in the field are commonly exposed to fluctuating light intensity, caused by variable cloud cover, self‐shading of leaves canopy and/or leaf movement due turbulence. In contrast C 3 plant species, only little is known about effects dynamic (DL) on photosynthesis and growth 4 plants. Two two monocot eudicot species were grown under steady or DL conditions with equal sum daily incident photon flux. We measured gas exchange, dry matter carbon isotope discrimination infer CO 2 bundle...
The initiation of stomata, microscopic valves in the epidermis higher plants that control gas exchange, requires a co-ordinated sequence asymmetric and symmetric divisions, which is under tight environmental developmental control. Arabidopsis leaves grown elevated photosynthetic photon flux density have stomata. STOMAGEN encodes an epidermal patterning factor produced mesophyll, our observations indicated irradiation stimulates expression. Our analysis gain loss function further detailed its...
Soil microbial physiology controls large fluxes of C to the atmosphere, thus, improving our ability accurately quantify in soil is essential. However, current methods determine metabolism require liquid water addition, which makes it practically impossible measure dry samples without stimulating growth and respiration (namely, "Birch effect"). We developed a new method based on vivo 18 O-water vapor equilibration minimize rewetting effects. This allows isotopic labeling direct addition. was...
Stomata exert control on fluxes of CO2 and water (H2 O) in the majority vascular plants thus are pivotal for planetary carbon H2 O. However, mosses, significance possible function sporophytic stomata not well understood, hindering understanding ancestral evolution these key structures land plants. Infrared gas analysis 13 labelling, with supporting data from gravimetry optical scanning electron microscopy, were used to measure assimilation exchange young, green, ± fully expanded capsules 11...
Cellulose delta18O and deltaD can provide insights on climates hydrological cycling in the distant past how these factors differ spatially. However, most studies of plant cellulose have used only one isotope, commonly delta18O, resulting difficulties partitioning variation precipitation vs. evaporative conditions that affect leaf water isotopic enrichment. Moreover, observations pronounced diurnal differences from conventional steady-state model predictions fractionation cast some doubt...
The pitcher plant Nepenthes ampullaria has an unusual growth pattern, which differs markedly from other species in the carnivorous genus Nepenthes. Its pitchers have a reflexed lid and sit above soil surface tighly packed 'carpet'. They contain significant amount of plant-derived materials, suggesting that this is partially herbivorous. We tested hypothesis benefits leaf litter utilization by increased photosynthetic efficiency sensu stricto cost/benefit model. Stable nitrogen isotope...
Winter barley is an economically important cereal crop grown in higher latitudes and altitudes where low temperatures represent environmental constraint limiting productivity. In this study changes proteome of leaves crowns a frost tolerant winter cv. Luxor relation to short long term periods cold followed by brief treatment were studied order disclose proteins responsible for the hardening process distinct plant tissues. The mentioned have been monitored using two dimensional difference gel...
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The strategies of Norway spruce [<i>Picea abies</i> (L.) Karst.] to increasing atmospheric CO<sub>2</sub> concentration (<i>C</i><sub>a</sub>) are not entirely clear. Here, we reconstructed centennial trajectories leaf internal (<i>C</i><sub>i</sub>) and intrinsic water-use efficiency (WUE<sub>i</sub>) from the amount <sup>13</sup>C in tree-ring cellulose. We collected 57 cores across elevations, soil, conditions central Europe. Generally, WUE<sub>i</sub> <i>C</i><sub>i</sub> increased over...
Acclimation of stomatal conductance to different CO2 and temperature regimes was determined in Chenopodium album L. plants grown at one three treatment conditions: 23�C 350 μmol mol-1 air; 34�C mol-1; 750 mol-1. Stomatal (gs) as a function intercellular (Ci) for each 25 35�C, these data were used estimate gains the feedback loops linking changes with net assimilation. Growth affected sensitivity stomata measurement pattern that influenced by CO2. more than doubled varying between 200 600...
Different behaviour of small groups stomata on a single leaf blade (stomatal patchiness) is reviewed. The occurrence stomatal patchiness depends plant species, age, position, environmental conditions,etc. possibility errors in conventional evaluation and non-stomatal (biochemical) limitations photosynthesis resulting from patchy closure analysed. consequences for water economy are discussed. A brief survey the techniques currently used detection quantification presented.
The first evidence of the existence gene-encoded Zn-binding peptides that sequester a substantial portion intracellular Zn in ectomycorrhizal fungi under natural conditions.
Stomatal density (SD) generally decreases with rising atmospheric CO2 concentration, Ca. However, SD is also affected by light, air humidity and drought, all under systemic signalling from older leaves. This makes our understanding of how Ca controls incomplete. study tested the hypotheses that internal concentration leaf, Ci, rather than Ca, cotyledons, as first plant assimilation organs, lack signal. Sunflower (Helianthus annuus), beech (Fagus sylvatica), arabidopsis (Arabidopsis thaliana)...
Abstract Explaining species geographic distributions by macroclimate variables is the most common approach for getting mechanistic insights into large‐scale diversity patterns and range shifts. However, species' traits influencing biophysical processes can produce a large decoupling from ambient air temperature, which seriously undermine biogeographical inference. We combined stable oxygen isotope theory with trait‐based to assess leaf temperature during carbon assimilation ( T L ) its...
Transpiration of cuticular membranes isolated from the lower stomatous surface Hedera helix (ivy) leaves was measured using a novel approach which allowed distinction to be made between gas phase diffusion (through stomatal pores) and solid (transport through polymer matrix membrane waxes) water molecules. This is based on principle that diffusivity vapour in can manipulated by different gases (helium, nitrogen, or carbon dioxide) while not affected. flow across pores ('stomatal...
The rootless, aquatic Utricularia species belong to the largest and most cosmopolitan carnivorous plant genus. Populations of plants are an important component many standing, nutrient-poor, humic waters. Carbon (C) allocation is aspect Utricularia's ecophysiology that has not been studied previously there considerable uncertainty about functional ecological benefit trap-associated microbial community potential role played by C exudation in enhancing plant-microbe interactions. A...
Stomata modulate the exchange of water and CO2 between plant atmosphere. Although stomatal density is known to affect diffusion into leaf thus photosynthetic rate, effect patterning on assimilation not fully understood. We used wild types Col-0 C24 mutants sdd1-1 tmm1 Arabidopsis thaliana, differing in pattern, study effects these variations both mesophyll conductance rate. Anatomical parameters stomata, temperature carbon isotope discrimination were also assessed. Our results indicate that...