A5052742087- Glioma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune Cell Function and Interaction
- Cytomegalovirus and herpesvirus research
- Systemic Lupus Erythematosus Research
- Cancer, Hypoxia, and Metabolism
- Immunodeficiency and Autoimmune Disorders
- Immune cells in cancer
- Microtubule and mitosis dynamics
- Angiogenesis and VEGF in Cancer
- Cell Adhesion Molecules Research
- MicroRNA in disease regulation
- CAR-T cell therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Nanoplatforms for cancer theranostics
- Diabetes and associated disorders
- Cancer Research and Treatments
- Chemokine receptors and signaling
- Neurogenesis and neuroplasticity mechanisms
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
University of Oslo
2015-2024
Akershus University Hospital
2024
Oslo University Hospital
2015-2024
University of Rijeka
2013-2018
University of Bergen
2003-2017
Haukeland University Hospital
2005-2013
Royal College of Surgeons in Ireland
2013
University College Dublin
2013
Laboratoire National de Santé
2012
Institute for Biomedicine
2008
Angiogenesis is regarded as a hallmark of cancer progression and it has been postulated that solid tumor growth depends on angiogenesis. At present, however, clear cell invasion can occur without angiogenesis, phenomenon particularly evident by the infiltrative malignant brain tumors, such glioblastomas (GBMs). In these amplification or overexpression wild-type (wt) truncated constitutively activated epidermal factor receptor (EGFR) are important events in GBM development, where complex...
Establishing clinically relevant animal models of glioblastoma multiforme (GBM) remains a challenge, and many commonly used cell line-based do not recapitulate the invasive growth patterns patient GBMs. Previously, we have reported formation highly tumour xenografts in nude rats from human However, implementing based on primary tissue requires that these can be sufficiently standardised with consistently high take rates. In this work, collected data kinetics material 29 biopsies xenografted...
Glioblastoma is the most frequent and malignant primary brain tumor with a poor prognosis. The translation of therapeutic strategies for glioblastoma from experimental phase into clinic has been limited by insufficient animal models, which lack important features human tumors. Lentiviral gene therapy an attractive option glioblastoma, we validated in clinically relevant model.We used rodent xenograft model that recapitulates invasive angiogenic to analyze transduction pattern efficacy...
Abstract Congenital HCMV infection is a leading infectious cause of long‐term neurodevelopmental sequelae. Infection newborn mice with mouse cytomegalovirus (MCMV) intraperitoneally well‐established model congenital human infection, which best recapitulates the hematogenous route virus spread to brain and subsequent pathology. Here, we used this investigate role, dynamics, phenotype CD8 + T cells in following mice. We show that infiltrate form pool tissue‐resident memory (T RM cells) persist...
Cysteine cathepsins play an important role in shaping the highly infiltrative growth pattern of human gliomas. We have previously demonstrated that activity cysteine is elevated invasive glioblastoma (GBM) cells vitro, part due to attenuation their endogenous inhibitors, cystatins. To investigate this relationship vivo, we established U87-MG xenografts non-obese diabetic (NOD)/severe combined immunodeficiency (SCID)-enhanced green fluorescent protein (eGFP) mice. Here, tumor correlated with...
Oligodendroglioma poses a biological conundrum for malignant adult human gliomas: it is tumor type that universally incurable patients, and yet, only few of the tumors have been established as cell populations in vitro or intracranial xenografts vivo. Their survival, thus, may emerge within specific environmental context. To determine fate oligodendroglioma an experimental model, we studied development anaplastic after implantation into enhanced green fluorescent protein (eGFP) positive...
Enhancing the germinal center (GC) reaction is a prime objective in vaccine development. Targeting of antigen to MHCII on APCs has previously been shown increase antibody responses, but underlying mechanism unclear. We have here investigated GC after targeting (i) defined model with T and B cells known specificity using adjuvant-free proteins, (ii) an infectious disease DNA vaccine. MHCII-targeting enhanced presentation peptide: APCs, increased numbers cells, TFH, plasma cells. Antibodies...
Background Glioblastoma multiforme (GBM) is the most aggressive type of malignant primary brain tumors in adults. Molecular and genetic analysis has advanced our understanding glioma biology, however mapping cellular composition tumor microenvironment crucial for pathology this dreaded cancer. In study we identified major cell populations attracted by using orthotopic rodent models human xenografts. Marker-specific, anatomical morphological analyses revealed a robust influx host cells into...
Glioblastoma multiforme (GBM), the most aggressive brain malignancy, is characterized by extensive cellular proliferation, angiogenesis, and single-cell infiltration into brain. We have previously shown that a xenograft model based on serial xenotransplantation of human biopsy spheroids in immunodeficient rodents maintains genotype phenotype original patient tumor. The present work further extends this for optical assessment tumor engraftment growth using bioluminescence imaging (BLI). A...
The B cell receptors (BCRs) for antigen express variable (V) regions that are enormously diverse, thus serving as markers on individual cells. V region-derived idiotypic (Id) peptides can be displayed pId:MHCII complexes cells recognition by CD4+ T It is not known if naive spontaneously display in vivo or BCR ligation required expression, thereby enabling collaboration between Id+ and Id-specific Here, using a mouse model, we show do readily detectable levels of pId:MHCII. However, Ag...
Targeting antigen to conventional dendritic cells (cDCs) can improve antigen-specific immune responses and additionally be used influence the polarization of responses. However, mechanisms by which this is achieved are less clear. To our understanding, we here evaluate molecular cellular requirements for CD4 + T cell antibody after immunization with Xcl1-fusion vaccines that specifically target cDC1s. induced an IgG2a/IgG2b-dominated response rapid Th1 both in vitro vivo . For comparison,...
The transduction patterns of recombinant adeno-associated virus serotype 1 (AAV1) and 6 (AAV6) vectors were assessed in human glioblastoma multiforme (GBM) cell lines, GBM biopsy spheroids, tumor xenografts growing nude rat brains. All the lines tested (A172, D37, GaMg, HF66, U373Mg) found to be permissive both AAV1 AAV6 vectors, thus displayed a pattern similar AAV2 vectors. For every line tested, efficiency by was better than isogenic isopromoter Transduction dependent on viral particle...
Humoral immunity relies on the efficient differentiation of memory B cells (MBCs) into antibody-secreting (ASCs). T helper (Th) signals upregulate cell receptor (BCR) signaling by potentiating Src family kinases through increasing CD45 phosphatase activity (CD45 PA).In this study, we show that high PA in MBCs enhances BCR and is essential for their effective ASC differentiation. Mechanistically, Th intensifying surface binding a ligand, Galectin-1. works as sensor help defines high-affinity...
Abstract Background Adeno‐associated viral (AAV) vectors are potent delivery vehicles for gene transfer strategies directed at the central nervous system (CNS), muscle and liver. However, comparatively few studies have described AAV‐mediated to tumor tissues. We previously demonstrated that while AAV2 Adenoviral (Ad) 5 similar broad host ranges in tumor‐derived cell lines, was able penetrate human glioblastoma biopsy spheroids xenografts more efficiently than Ad vectors. These results...
Abstract We have examined the spread and antitumor efficacy of an oncolytic herpes simplex virus-1–based vector (G207) in glioblastoma biopsy spheroids vitro vivo after local delivery to corresponding intracranial xenografts. Spheroids from three patients were infected with increasing doses G207 transgene expression was quantified. Other followed for 10 days assess cytotoxic effects. For study, grafted intracerebrally into Rowett nude rats. The resulting highly infiltrative xenografts...