A5052827585- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- DNA and Nucleic Acid Chemistry
- Microtubule and mitosis dynamics
- Fungal and yeast genetics research
- Cancer therapeutics and mechanisms
- Photosynthetic Processes and Mechanisms
- Carcinogens and Genotoxicity Assessment
- Bacterial Genetics and Biotechnology
- Mitochondrial Function and Pathology
- RNA modifications and cancer
- Plant Genetic and Mutation Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Polyomavirus and related diseases
- Genetic Mapping and Diversity in Plants and Animals
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- PARP inhibition in cancer therapy
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
Institut de Génétique Humaine
2016-2025
Université de Montpellier
2016-2025
Centre National de la Recherche Scientifique
2013-2024
Centre National pour la Recherche Scientifique et Technique (CNRST)
2020
Institut Curie
1995-1999
Université Paris-Sud
1992-1994
Laboratoire de Microbiologie et Génétique Moléculaires
1984-1987
National Institutes of Health
1987
Meiotic recombination events cluster into narrow segments of the genome, defined as hotspots. Here, we demonstrate that a major player for hotspot specification is Prdm9 gene. First, two mouse strains differ in usage are polymorphic zinc finger DNA binding array PRDM9. Second, human consensus PRDM9 allele predicted to recognize 13-mer motif enriched at hotspots; this specificity verified by vitro studies. Third, allelic variants fingers significantly associated with variability genome-wide...
Understanding the extent of genomic transcription and its functional relevance is a central goal in genomics research.However, detailed genomewide investigations transcriptome complexity major mammalian organs have been scarce.Here, using extensive RNA-seq data, we show that genome substantially more widespread testis than other across representative mammals.Furthermore, reveal meiotic spermatocytes especially postmeiotic round spermatids remarkably diverse transcriptomes, which explains...
A partner protein for meiotic snip Eukaryotes generate germ cells through recombination. This process initiates breaks in genomic DNA catalyzed by the SPO11 protein. Vrielynck et al. and Robert discover that SPO11, like topoisomerase VI enzymes, interacts with a (see Perspective Bouuaert Keeney). is required proper recombination found wide range of eukaryotes, suggesting it universal feature essential step. Science , this issue p. 939 943 ; see also 916
Meiotic recombination is initiated by the programmed induction of DNA double-strand breaks (DSBs) catalyzed evolutionarily conserved Spo11 protein. Studies in yeast have shown that DSB formation requires several other proteins, role and conservation which remain unknown. Here we show two these Saccharomyces cerevisiae Mei4 Rec114, are most eukaryotes. −/− mice deficient meiotic formation, thus showing functional mice. Cytological analyses reveal that, mice, MEI4 localized discrete foci on...
In mammals, the synaptonemal complex is a structure required to complete crossover recombination. Although suggested by cytological work, in vivo links between structural proteins of and recombination process have not previously been made. The central element traversed DNA at sites presents logical place look for interactions these components. There are four known proteins, three which mutated. Here, we set creating null mutation Syce1 gene mouse. resulting disruption synapsis animals has...
The molecular chaperone Hsp90 has been found to be essential for viability in all tested eukaryotes, from the budding yeast Drosophila. In mammals, two genes encode highly similar and functionally largely redundant isoforms Hsp90α Hsp90β. Although they are co-expressed most if not cells, their relative levels vary between tissues during development. Since mouse embryos lacking Hsp90β die at implantation, despite fact that inhibitors being as anti-cancer agents relatively well tolerated,...
Background Spermatogenesis is a complex biological process that requires highly specialized control of gene expression. In the past decade, small non-coding RNAs have emerged as critical regulators expression both at transcriptional and post-transcriptional level. DICER1, an RNAse III endonuclease, essential for biogenesis several classes RNAs, including microRNAs (miRNAs) endogenous interfering (endo-siRNAs), but also degradation toxic transposable elements. this study, we investigated to...
Highlights•We report a PRDM9 methyltransferase domain structure in complex with H3K4me2 and AdoHcy•H3K4 is genuine substrate of PRDM9•PRDM9 possesses mono-, di-, trimethylation activities•The PR/SET undergoes conformational changes during catalysisSummaryPRDM9, histone lysine methyltransferase, key determinant the localization meiotic recombination hot spots humans mice only vertebrate protein known to be involved hybrid sterility. Here, we crystal H3 peptide dimethylated on 4 (H3K4me2)...
Programmed formation of DNA double-strand breaks (DSBs) initiates the meiotic homologous recombination pathway. This pathway is essential for proper chromosome segregation at first division and fertility. Meiotic DSBs are catalyzed by Spo11. Several other proteins DSB formation, including three evolutionarily conserved identified in Saccharomyces cerevisiae (Mer2, Mei4, Rec114). These S. their mouse orthologs (IHO1, MEI4, REC114) co-localize on axes chromosomes, IHO1 MEI4 formation. Here, we...
In many eukaryotes, meiotic recombination occurs preferentially at discrete sites, called hotspots. various lineages, hotspots are located in regions with promoter-like features and evolutionarily stable. Conversely, some mammals, driven by PRDM9 that targets away from promoters. Paradoxically, induces the self-destruction of its this triggers an ultra-fast evolution mammalian is ancestral to all animals, suggesting a critical importance for program, but has been lost lineages surprisingly...