A5019944916- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Aortic Disease and Treatment Approaches
- Microtubule and mitosis dynamics
- Aortic aneurysm repair treatments
- Reproductive Biology and Fertility
- Congenital Heart Disease Studies
- Toxic Organic Pollutants Impact
- Per- and polyfluoroalkyl substances research
- Cardiac Valve Diseases and Treatments
- Renal and Vascular Pathologies
- Chromosomal and Genetic Variations
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Effects and risks of endocrine disrupting chemicals
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Birth, Development, and Health
- Water Treatment and Disinfection
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Cardiac, Anesthesia and Surgical Outcomes
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
University of Illinois Urbana-Champaign
2019-2025
Beijing Anzhen Hospital
2016-2024
Capital Medical University
2016-2024
Urbana University
2024
Beijing Institute of Water
2023-2024
Capital University
2021-2022
Chongqing University of Posts and Telecommunications
2021
Howard Hughes Medical Institute
2012-2018
University of California, Davis
2011-2018
Colorado State University
2011-2012
Male germ cell development requires precise regulation of gene activity in a cell-type and stage-specific manner, with perturbations expression during spermatogenesis associated infertility. Here, we use steady-state, nascent single-cell RNA sequencing strategies to comprehensively characterize across male populations, dissect the mechanisms control provide new insights towards therapy. We discover requirement for pausing Polymerase II (Pol II) at earliest stages sperm differentiation...
Proteasomes and SUMO wrestle chromosomes Meiosis is the double cell division that generates haploid gametes from diploid parental cells. Pairing of homologous during first meiotic ensures each gamete receives a complete set chromosomes. The proteasome, on other hand, molecular machine degrades proteins tagged for destruction within (see Perspective by Zetka). Ahuja et al. show proteasome also involved in ensuring pair with meiosis. Rao (small ubiquitin-like modifier) protein, ubiquitin,...
The intimate synapsis of homologous chromosome pairs (homologs) by synaptonemal complexes (SCs) is an essential feature meiosis. In many organisms, and recombination are interdependent: promotes SC formation SCs required for crossing-over. Moreover, several studies indicate that initiation assembly occurs at sites where crossovers will subsequently form. However, recent analyses in budding yeast fruit fly imply a special role centromeres the formation. addition, yeast, persistent...
Single-nucleotide substitutions and small in-frame insertions or deletions identified in human breast cancer susceptibility genes BRCA1 BRCA2 are frequently classified as variants of unknown clinical significance (VUS) due to the availability very limited information about their functional consequences. Such can most reliably be pathogenic non-pathogenic based on data co-segregation with affected families and/or co-occurrence a mutation. Biological assays that examine effect protein function...
Abstract Objectives Per‐ and polyfluoroalkyl substances (PFAS) are man‐made chemicals that widely used in various products. PFAS characterized by their fluorinated carbon chains make them hard to degrade bioaccumulate human animals. Toxicological studies have shown toxic effects: cytotoxicity, immunotoxicity, neurotoxicity, reproductive toxicity. However, it is still unclear how the structures of PFAS, such as carbon‐chain length functional groups, determine Methods Results By using a...
Chromosome structure is complex, and many aspects of its organization remain poorly understood. Measuring chromosome stiffness offers valuable insight into their structural properties. In this study, we analyzed the chromosomes from metaphase I (MI) II (MII) oocytes. Our results revealed a ten-fold increase in (Young’s modulus) MI compared to somatic chromosomes. Furthermore, MII was lower than that We examined role meiosis-specific cohesin complexes regulating stiffness. Surprisingly, three...
Chromosome structure is complex, and many aspects of chromosome organization are still not understood. Measuring the stiffness chromosomes offers valuable insight into their structural properties. In this study, we analyzed from metaphase I (MI) II (MII) oocytes. Our results revealed a tenfold increase in (Young’s modulus) MI compared to somatic chromosomes. Furthermore, MII was found be lower than that We examined role meiosis-specific cohesin complexes regulating stiffness. Surprisingly,...
This cohort study collected the clinical data of patients who underwent off-pump coronary artery bypass grafting (OPCABG) during hospitalization to observe occurrence postoperative atrial fibrillation (POAF), construct a POAF prediction model for CABG based on left diameter (LAD), and assist clinicians in making better medical decisions.In this study, all had no prior history arrhythmia received isolated OPCABG between May 1, 2021, February 2022, at Beijing Anzhen Hospital Affiliated Capital...
This study is to investigate the influence of hemodynamics on Stanford type-A aortic dissection with different tear size and location, provide some support for relationships between risks (rupture, reverse tearing further tearing) location clinical treatment.Four numerical models were established, tears. The ratio area entry re-entry tears(RA) various within model; while, in distal descending aorta are fixed. In model A11 A21, tears located near ascending aorta. RA these 1 2, respectively;...
In mammalian species, females are born with a number of oocytes exceeding what they release via ovulation. humans, an average girl is over thousand times more than she will ovulate in her lifetime. The reason for having such excessive neonatal female ovary currently unknown. However, it well established that the oocyte decreases throughout entire lifetime until loses them all. this review, data published past 80 years were used to assess current knowledge regarding changing humans and mice,...
Spermatogenesis is a biological process within the testis that produces haploid spermatozoa for continuity of species. Sertoli cells are somatic in seminiferous epithelium orchestrate spermatogenesis. Cyclic reorganization cell actin cytoskeleton vital spermatogenesis, but underlying mechanism remains largely unclear. Here, we report RNA-binding protein PTBP1 controls by programming alternative splicing regulators. This control enables ectoplasmic specializations, actin-based adhesion...
Unlike mild DNA damage exposure, repair (DDR) is reported to be ineffective in full-grown mammalian oocytes exposed moderate or severe damage. The underlying mechanisms of this weakened DDR are unknown. Here, we show that leads aneuploidy. Our data reveal DNA-damaged have an altered, closed, chromatin state, and suggest the failure damaged could due inability proteins access loci. also demonstrate that, unlike somatic cells, mouse porcine fail activate autophagy response double-strand...
Abstract Di-(2-ethylhexyl) phthalate (DEHP) is a chemical that widely used as plasticizer. Exposure to DEHP has been shown alter ovarian function in humans. Additionally, foods high fat content, regularly found the western diet, have be another potential disruptor of fetal function. Due DEHP’s lipophilicity, high-fat can easily contaminated. Therefore, exposure and diet are both health concerns, especially pregnant women, effects these exposures on oocyte quality quantity should elucidated....
Meiotic arrest is a common cause of human male infertility, but the causes this are poorly understood. Transactive response DNA-binding protein 43 kDa (TDP-43) highly expressed in spermatocytes preleptotene and pachytene stages meiosis. TDP-43 linked to several neurodegenerative disorders wherein its nuclear clearance accompanied by cytoplasmic aggregates underlies neurodegeneration. Exploring functional requirement for spermatogenesis first time, we show here that conditional KO (cKO)...