A5015121406- Complement system in diseases
- Cardiac Fibrosis and Remodeling
- Mitochondrial Function and Pathology
- Clostridium difficile and Clostridium perfringens research
- Respiratory Support and Mechanisms
- Blood groups and transfusion
- Immune Cell Function and Interaction
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Lymphoma Diagnosis and Treatment
- Liver Disease Diagnosis and Treatment
- Hemoglobinopathies and Related Disorders
- Metabolism and Genetic Disorders
- Long-Term Effects of COVID-19
- Endoplasmic Reticulum Stress and Disease
- T-cell and B-cell Immunology
- Neonatal Respiratory Health Research
- RNA and protein synthesis mechanisms
- Erythrocyte Function and Pathophysiology
- Microscopic Colitis
- Amyotrophic Lateral Sclerosis Research
- Helicobacter pylori-related gastroenterology studies
- Monoclonal and Polyclonal Antibodies Research
- Sperm and Testicular Function
- Lymphatic System and Diseases
- Metabolomics and Mass Spectrometry Studies
University of Virginia
2016-2025
University of Virginia Health System
2014-2024
Eijkman Institute for Molecular Biology
2002-2008
National Institutes of Health
2003
Osaka University
1990-1999
Siriraj Hospital
1999
Mahidol University
1999
Hyogo Medical University
1997
Osaka Medical and Pharmaceutical University
1997
Nagoya University
1993
Highlights•IL-25 is suppressed during human and murine Clostridium difficile infection (CDI)•Restoration of IL-25 reduces mortality morbidity CDI•Depletion or genetic deletion eosinophils negates IL-25-mediated protection•IL-25-induced eosinophilia maintains integrity the intestinal barrier CDISummaryClostridium (CDI) most common cause hospital-acquired in United States. Host susceptibility severity are influenced by disruption microbiota immune response. However, how regulate responses to...
Autoimmune responses to meiotic germ cell antigens (MGCA) that are expressed on sperm and testis occur in human infertility after vasectomy. Many MGCA also as cancer/testis (CTA) cancers, but the tolerance status of has not been investigated. considered be uniformly immunogenic nontolerogenic, prevailing view posits sequestered behind Sertoli barrier seminiferous tubules. Here, we have shown only some murine sequestered. Nonsequestered MCGA (NS-MGCA) egressed from normal tubules, evidenced...
Abstract Clostridium difficile ( C. ) incidence has tripled over the past 15 years and is attributed to emergence of hypervirulent strains. While it clear that toxins cause damaging colonic inflammation, immune mechanisms protecting from tissue damage require further investigation. Through a transcriptome analysis, we identify IL-33 as an target upregulated in response . We demonstrate prevents -associated mortality epithelial disruption independently bacterial burden or toxin expression....
Rationale: Immature control of breathing is associated with apnea, periodic breathing, intermittent hypoxemia, and bradycardia in extremely preterm infants. However, it not clear if such events independently predict worse respiratory outcome. Objectives: To determine analysis cardiorespiratory monitoring data can unfavorable outcomes at 40 weeks postmenstrual age (PMA) other outcomes, as bronchopulmonary dysplasia 36 PMA. Methods: The Prematurity-related Ventilatory Control (Pre-Vent) study...
Austronesian is a linguistic family spread in most areas of the Southeast Asia, Pacific Ocean, and Indian Ocean. Based on their similarity, this included Malayo-Polynesians Taiwan aborigines. The similarity also led to controversial hypothesis that homeland all Malayo-Polynesians, has been debated by ethnologists, linguists, archaeologists, geneticists. It well accepted Eastern Austronesians (Micronesians Polynesians) derived from Western (Island Asians Taiwanese), Daic populations mainland...
Clostridium difficile is currently the leading cause of hospital-acquired infections in United States. Here, we observed increased interleukin 23 (IL-23) protein levels human colon biopsy specimens positive for C. toxins, compared with negative controls (P = .008) We also investigated role IL-23 during infection, using 2 distinct murine models. Mice lacking signaling had a significant increase survival (100% [12 mice]), control mice (16.7%–50% mice]). These data suggest new potential drug...
The disease severity of Entamoeba histolytica infection ranges from asymptomatic to life-threatening. Recent human and animal data implicate the gut microbiome as a modifier E. virulence. Here we have explored association with susceptibility amebiasis in infants mouse model amebic colitis. Dysbiosis occurred symptomatic children, evidenced by lower Shannon diversity index microbiota. To test if dysbiosis was cause susceptibility, wild type C57BL/6 mice (which are innately resistant...
ABSTRACT The role of leptin in the mucosal immune response to Clostridium difficile colitis, a leading cause nosocomial infection, was studied humans and murine model. Previously, mutation receptor for ( LEPR ) shown be associated with susceptibility infectious colitis liver abscess due Entamoeba histolytica as well bacterial peritonitis. Here we discovered that European Americans homozygous same Q223R (rs1137101), known result decreased STAT3 signaling, were at increased risk C. infection...
Abstract Escherichia coli K12 strain W3110/SM bearing a plasmid containing the traT gene (traT+ strain) was more resistant to bactericidal activity of guinea pig serum than same this without (traT- strain). A murine mAb generated against synthetic TraT peptide (86-99). This antibody reacted only with denatured protein, but it used for monitoring protein by immunoblotting during purification protein. Six were then partially purified from solubilized membrane fraction traT+ strain. These...
The development and validation of a multiscopic near-infrared fluorescence (NIRF) probe, cinnamoyl-F-(D)L-F-(D)L-F-PEG-cyanine7 (cFlFlF-PEG-Cy7), that targets formyl peptide receptor on neutrophils using mice ear inflammation model is described. Acute was induced in by topical application phorbol-12-myristate-13-acetate to left ears 24 hours before probe administration. Noninvasive NIRF imaging longitudinally performed up following injection. vivo neutrophil-targeting specificity the...
Abstract Cancer cell dissemination to sentinel lymph nodes is associated with poor patient outcomes, particularly in breast cancer. The process by which cancer cells egress from the primary tumor upon interfacing lymphatic vasculature complex and driven dynamic interactions between stromal cells, including cancer-associated fibroblasts (CAF). matricellular protein periostin can distinguish CAF subtypes increased desmoplasia disease recurrence patients. However, as secreted,...
C5 convertase of the classical complement pathway is a protein complex consisting C4b, C2a, and C3b.Within this C3b binds to C4b via an ester linkage.We now present evidence that covalent C3b-binding site on human Ser at position 1217 C4.We also show formation covalently linked C4beC3b occurs in mouse system 1213 which homologous Ser-1217 C4.Therefore, binding single specific within likely common phenomenon mammalian system.Specific noncovalent association metastable with would occur first,...
SUMMARY The study of spermatogenesis requires accurate identification the stages cycle seminiferous epithelium. A stage refers to unique association germ cell types at a particular phase development, as seen in cross‐sectioned tubule. Stage‐identification, however, is daunting task. There are 12 represented mouse Stages typically identified on basis morphology developing acrosome spermatids. Although characteristic features well‐documented ultrastructure images, reagent that can highlight...
Inhibition of mitochondrial function and the subsequent generation oxidative stress are strongly suggested to underlie MPTP/MPP+-induced neurotoxicity, which has been used extensively as a model for Parkinson disease. In present study we have examined hypothesis that MPP+ targets endoplasmic reticulum. Because rabbits possess more genetic similarities primates than rodents selected this animal system our neurotoxicity studies. was administered directly into brain New Zealand white via...
C5 convertase of the alternative complement pathway is a trimolecular complex consisting two molecules C3b and one molecule Bb. We previously proposed model in which second binds covalently to first bearing Bb, each linked dimer, resulting its appropriate presentation catalytic site on In present study, we purified dimer reconstituted with factors B D obtain evidence support this model. An insoluble glucan, OMZ-176, was incubated human serum activate allow formation surface glucan then...