A5083807236- Monoclonal and Polyclonal Antibodies Research
- Immunodeficiency and Autoimmune Disorders
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Immune Cell Function and Interaction
- Metabolomics and Mass Spectrometry Studies
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- Wireless Signal Modulation Classification
- Microbial Metabolic Engineering and Bioproduction
- Cancer Genomics and Diagnostics
- Cancer Immunotherapy and Biomarkers
- Full-Duplex Wireless Communications
- Long-Term Effects of COVID-19
- Material Properties and Processing
- Radar Systems and Signal Processing
- Respiratory viral infections research
- Bacterial Genetics and Biotechnology
- HIV Research and Treatment
- Protein purification and stability
- Surface Roughness and Optical Measurements
- Immune cells in cancer
- RNA and protein synthesis mechanisms
University of Virginia Cancer Center
2025
University of Virginia
2020-2025
Carter Center
2023-2025
Ragon Institute of MGH, MIT and Harvard
2019-2023
Charlottesville Medical Research
2022
United States Air Force Academy
2022
Karolinska Institutet
2021
Science for Life Laboratory
2021
Massachusetts Institute of Technology
2018-2021
Georgia Institute of Technology
2010-2016
Variations in the RF chain of radio transmitters can be used as a signature to uniquely associate wireless devices with given transmission. Previous approaches, which have varied from transient analysis machine learning, do not provide verifiable accuracy, is essential for admissibility methods court. Here we detail first step toward model-based approach, uses statistical models transmitter components that are amenable analysis. Algorithms based on signal processing developed exploit...
We are rapidly approaching a future in which cancer patient digital twins will reach their potential to predict prevention, diagnosis, and treatment individual patients. This be realized based on advances high performance computing, computational modeling, an expanding repertoire of observational data across multiple scales modalities. In 2020, the US National Cancer Institute, Department Energy, through trans-disciplinary research community at intersection advanced computing research,...
N-linked glycosylation affects the potency, safety, immunogenicity, and pharmacokinetic clearance of several therapeutic proteins including monoclonal antibodies. A robust control strategy is needed to dial in appropriate profile during course cell culture processes accurately. However, N-glycosylation dynamics remains insufficiently understood owing lack integrative analyses factors that influence dynamics, sugar nucleotide donors, glycosyltransferases, glycosidases. Here, an approach...
With an estimated 25% of the global population infected with Mycobacterium tuberculosis ( Mtb ), (TB) remains a leading cause death by infectious diseases. Humoral immunity following TB treatment is largely uncharacterized, and antibody profiling could provide insights into disease resolution. Here we focused on distinctive TB-specific serum features in active (ATB) compared them latent infection (LTBI) or treated ATB (txATB). As expected, di-galactosylated glycan structures (lacking sialic...
Abstract Preterm newborns are more likely to suffer from infectious diseases at birth compared children delivered term. Whether this is due compromised cellular, humoral, or organ-specific development remains unclear. To begin define whether maternal–fetal antibody transfer profiles differ across preterm (PT) and fullterm (FT) infants, the overall quantity functional quality of an array 24 vaccine-, endemic pathogen-, common antigen-specific antibodies were assessed a cohort 11 PT 12...
ABSTRACT Tumor clearance by T cells is impaired insufficient tumor antigen recognition, infiltration, and the immunosuppressive microenvironment (TME). Although targeted cell therapy circumvents failures in suppression TME failure to infiltrate can hinder these cells. Checkpoint inhibitors (CPI) promises reverse be combined with bispecific antibody armed (BATs) improve clinical outcomes. CPIs require target pathway of inhibition active elicit a therapeutic response. We hypothesize that...
ABSTRACT The pancreatic ductal adenocarcinoma (PDAC) tumor microenvironment contains hypoxic tissue subdomains and cancer-associated fibroblasts (CAFs) of multiple subtypes that play tumor-promoting -restraining roles. Here, we demonstrate hypoxia promotes an inflammatory-like CAF phenotype CAFs selectively promote epithelial-mesenchymal transition (EMT) in PDAC cancer cells through growth factor-mediated cell crosstalk. By analyzing patient single-cell transcriptomics conducting inhibitor...
Abstract Background Human immunodeficiency virus (HIV)–exposed, uninfected (HEU) children have a higher risk of severe infection, but the causes are poorly understood. Emerging data point to altered antibody transfer in women with HIV (WHIV); however, specific perturbations and influence antiretroviral therapy (ART) viremia remain unclear. Methods We evaluated antigen-specific transplacental across 14 antigens paired maternal umbilical cord plasma from 352 Ugandan women; 176 were WHIV taking...
Studies have demonstrated the protective role of antibodies against malaria. Young children are known to be particularly vulnerable malaria, pointing evolution naturally acquired clinical immunity over time. However, whether changes in antibody functionality track with acquisition malaria remains incompletely understood.
Variations in the RF chain of radio transmitters can be used as a signature to uniquely associate wireless devices with given transmission. Previous approaches, which have varied from transient analysis machine learning, do not provide verifiable accuracy. Here, we detail first step toward model-based approach. In particular exploit differences nonlinearities input/output (I/O) characteristics power amplifiers modeled Volterra series and develop algorithms for deciding origin message...
While the RV144 HIV vaccine trial led to moderately reduced risk of acquisition, emerging data from HVTN702 point critical need reexamine RV144-based correlates protection. in RV144, induction V2-binding, non-IgA, IgG3 antibody responses with nonneutralizing functions were linked infection, interactions between these signatures remain unclear. Thus, here we comprehensively profile humoral immune response 300 vaccinees decipher relationships biomarkers We found that vaccine-specific IgG1,...
Transplacental antibody transfer is crucially important in shaping neonatal immunity. Recently, prenatal maternal immunization has been employed to boost pathogen-specific immunoglobulin G (IgG) the fetus. Multiple factors have implicated transfer, but how these key regulators work together elicit selective pertinent engineering vaccines for mothers optimally immunize their newborns. Here, we present first quantitative mechanistic model uncover determinants of placental and inform...
The estimation of parameters in even moderately large biological systems is a significant challenge. This challenge greatly exacerbated if the mathematical formats appropriate process descriptions are unknown. To address this challenge, method dynamic flux (DFE) was proposed for analysis metabolic time series data. Under ideal conditions, first phase DFE yields numerical representations all fluxes within pathway system, either as values at each point or plots against their substrates and...
The dairy bacterium Lactococcus lactis has to master a complicated task. It must control its essentially linear glycolytic pathway in such fashion that, when the substrate, glucose, runs out, it retains enough phosphoenolpyruvate and fructose-1,6-bisphosphate be able restart glycolysis as soon new glucose becomes available. Although is arguably best-studied metabolic pathway, details L. are still unclear, is, particular, not understood how manages stop-and-start primary purpose of this paper...
MHC class I (MHC-I) loss is frequent in non-small cell lung cancer (NSCLC) rendering tumor cells resistant to T lysis. NK kill MHC-I-deficient cells, and although previous work indicated their presence at NSCLC margins, they were functionally impaired. Within, we evaluated whether CD8 infiltration activation vary with MHC-I expression.
The variable etiology of persistent breathlessness after COVID-19 have confounded efforts to decipher the immunopathology lung sequelae. Here, we analyzed hundreds cellular and molecular features in context discrete pulmonary phenotypes define systemic immune landscape post-COVID disease. Cluster analysis physiology measures highlighted two restrictive disease that differed by their impaired diffusion severity fibrosis. Machine learning revealed marked CCR5+CD95+ CD8+ T-cell perturbations...
Background Major histocompatibility complex class I (MHC-I) loss is frequent in non-small cell lung cancer (NSCLC) rendering tumor cells resistant to T lysis. NK kill MHC-I-deficient cells, and although previous work indicated their presence at NSCLC margins, they were functionally impaired. Within, we evaluated whether CD8 infiltration activation vary with MHC-I expression. Methods We used single-stain immunohistochemistry (IHC) Kaplan-Meier analysis test the effect of on overall...
This article and the companion paper use computational systems modeling to decipher complex coordination of regulatory signals controlling glycolytic pathway in dairy bacterium Lactococcus lactis. In this first article, development a comprehensive kinetic dynamic model is described. The based on vivo NMR data that consist concentration trends key metabolites cofactors. structure parameter values are identified with customized optimization strategy uses as its core method flux estimation. For...
<h3>Background</h3> Cell and bispecific antibody (biAb) therapies have had limited success in solid tumors. Arming ex vivo-activated T cells (ATC) with tumor antigen-targeting biabs creates a living 'drug' that exhibits non-MHC restricted biab-directed cytotoxicity is capable of repeated killing cells. BiAb armed ATC (BATs) therapy has improved survival patients metastatic breast, prostate, pancreatic cancer as well neuroblastoma without dose limiting toxicities. Understanding mechanisms...