A5074919412- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- Hormonal and reproductive studies
- Long-Term Effects of COVID-19
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- Gut microbiota and health
- T-cell and B-cell Immunology
- Urticaria and Related Conditions
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- Immunodeficiency and Autoimmune Disorders
- Herpesvirus Infections and Treatments
- Kawasaki Disease and Coronary Complications
- Diabetes and associated disorders
- Vagus Nerve Stimulation Research
- COVID-19 Impact on Reproduction
- Neonatal Respiratory Health Research
- Olfactory and Sensory Function Studies
- Animal Virus Infections Studies
- Fibromyalgia and Chronic Fatigue Syndrome Research
Karolinska Institutet
2016-2024
Science for Life Laboratory
2018-2023
IrsiCaixa
2010-2016
Universitat Autònoma de Barcelona
2011-2016
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2016
Hospital Universitari Germans Trias i Pujol
2010-2014
Red de Investigación en Sida
2011
<h2>Summary</h2> Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is typically very mild and often asymptomatic in children. A complication the rare multisystem inflammatory children (MIS-C) associated with COVID-19, presenting 4–6 weeks after as high fever, organ dysfunction, strongly elevated markers of inflammation. The pathogenesis unclear but has overlapping features Kawasaki disease suggestive vasculitis a likely autoimmune etiology. We apply systems-level...
Epidemiological data suggest that early life exposures are key determinants of immune-mediated disease later in life. Young children also particularly susceptible to infections, warranting more analyses immune system development Such mostly have been performed mouse models or human cord blood samples, but these cannot account for the complex environmental influencing newborns after birth. Here, we longitudinal 100 newborn children, sampled up 4 times during their first 3 months From μL...
Immune-microbe interactions early in life influence the risk of allergies, asthma, and other inflammatory diseases. Breastfeeding guides healthier immune-microbe relationships by providing nutrients to specialized microbes that turn benefit host's immune system. Such bacteria have co-evolved with humans but are now increasingly rare modern societies. Here we show a lack bifidobacteria, particular depletion genes required for human milk oligosaccharide (HMO) utilization from metagenome, is...
A blood cell protein-expression atlas Genome-wide analyses are increasingly providing resources for advances in basic and applied biomedical science. Uhlen et al. performed a global expression analysis of human types integrated this data with across all major tissues organs the protein atlas. This comprehensive compendium allows classification protein-coding genes regard to their tissue- cell-type distribution. Science , issue p. eaax9198
HighlightsImmunomonitoring from acute to recovery phase COVID-19An IFNγ-eosinophil axis precedes lung hyperinflammationBasophils modulate SARS-CoV-2 IgG responsesA shared trajectory of immunological in COVID-19SummarySevere disease is characterized by vigorous inflammatory responses the lung, often with a sudden onset after 5–7 days stable disease. Efforts this hyperinflammation and associated respiratory distress syndrome rely on unraveling immune cell interactions cytokines that drive such...
Several autoimmune features occur during coronavirus disease 2019 (COVID-19), with possible implications for course, immunity, and pathology. In this study, we longitudinally screened clinically relevant systemic autoantibodies to assess their prevalence, temporal trajectory, association comorbidities, severity of COVID-19.We performed highly sensitive indirect immunofluorescence assays detect antinuclear antibodies (ANA) antineutrophil cytoplasmic (ANCA), along serum proteomics virome-wide...
ObjectivesThe objective of this study was to inform public health actions limit first-line ART failure and HIV drug resistance in Mozambique.
The objective of this study was to define the natural genotypic variation HIV-1 integrase gene across Europe for epidemiological surveillance strand-transfer inhibitor (InSTI) resistance. This a multicentre, cross-sectional within European SPREAD HIV resistance programme. A representative set 300 samples selected from 1950 naive HIV-positive subjects newly diagnosed in 2006–07. prevalence InSTI evaluated using quality-controlled baseline population sequencing integrase. Signature...
SUMMARY SARS-CoV-2 infection is typically very mild and often asymptomatic in children. A complication the rare Multisystem Inflammatory Syndrome Children (MIS-C) associated with COVID-19, presenting 4-6 weeks after as high fever, organ dysfunction strongly elevated markers of inflammation. The pathogenesis unclear but has overlapping features Kawasaki disease suggestive vasculitis a likely autoimmune etiology. We apply systems-level analyses blood immune cells, cytokines autoantibodies...
Abstract Preterm newborns are more likely to suffer from infectious diseases at birth compared children delivered term. Whether this is due compromised cellular, humoral, or organ-specific development remains unclear. To begin define whether maternal–fetal antibody transfer profiles differ across preterm (PT) and fullterm (FT) infants, the overall quantity functional quality of an array 24 vaccine-, endemic pathogen-, common antigen-specific antibodies were assessed a cohort 11 PT 12...
In order to design strategies for eradication of HIV-1 from infected individuals, detailed insight into the reservoirs that persist in patients on suppressive antiretroviral therapy (ART) is required. this regard, most studies have focused integrated (proviral) DNA forms cells circulating blood. However, majority proviral replication-defective and archival, as such, has limited ability reveal dynamics viral population persists ART. contrast, extrachromosomal (episomal) labile a consequence...
Objective To explore the potential of deep HIV-1 sequencing for adding clinically relevant information relative to viral population in heavily pre-treated HIV-1-infected subjects. Methods In a proof-of-concept study, was compared individuals with previous triple-class virological failure who also developed virologic salvage therapy including, at least, darunavir, tipranavir, etravirine or raltegravir. Viral susceptibility inferred before initiation and using genotypes interpreted HIVdb, Rega...
Ultrasensitive human immunodeficiency virus type 1 (HIV-1) genotyping predicts salvage antiretroviral therapy (ART) outcomes better than Sanger sequencing. A genotypic sensitivity score <3 by 454 is an independent predictor of virological failure to ART. HIV-1 drug resistance testing may improve ART outcomes. Background. The clinical relevance ultrasensitive in treatment (ART)-experienced individuals remains unknown. Methods. This was a retrospective, multicentre, cohort study...
SUMMARY The immune response to SARS-CoV2 is under intense investigation, but not fully understood att this moment. Severe disease characterized by vigorous inflammatory responses in the lung, often with a sudden onset after 5–7 days of stable disease. Efforts modulate hyperinflammation and associated acute respiratory distress syndrome, rely on unraveling cell interactions cytokines that drive such responses. Systems-level analyses are required simultaneously capture all populations many...
To evaluate the safety and efficacy of switching third drug antiretroviral treatment to maraviroc in aviraemic subjects infected with R5 HIV. This is a pilot, prospective, randomized clinical trial (ClinicalTrials ID: NCT00966329). Eighty HIV-1-infected adults on stable for ≥1 year no resistance were screened presence non-R5 HIV by triplicate proviral V3 population sequencing. From them, 30 HIV-1 1 : switch non-nucleoside reverse transcriptase inhibitor or ritonavir-boosted protease (n = 15)...
The clinical relevance of mutations in the connection subdomain and ribonuclease (RNase) H domain HIV-1 reverse transcriptase (RT) is uncertain.The risk virological failure to nonnucleoside RT inhibitor (NNRTI)-based antiretroviral therapy (ART) was evaluated NNRTI-naive patients who started NNRTIs EuroSIDA study after July 1997 according preexisting substitutions RNase RT. An observed association between A376S further investigated by testing vitro NNRTI susceptibility single site-directed...
In most patients, current antiretroviral therapy (ART) regimens can rapidly reduce plasma viral load. However, even after years of effective treatment, a significant proportion patients show residual viremia below the clinical detection limit. Although might be associated with increased chronic immune activation and morbidity, its origin potential role in replenishment reservoir during suppressive ART is not completely understood. We performed an in-depth genetic analysis total episomal...
Massive amounts of metagenomics data are currently being produced, and in all such projects a sizeable fraction the resulting shows no or little homology to known sequences. It is likely that this contains novel viruses, but identification challenging since they frequently lack viruses. To overcome problem, we developed strategy detect ORFan protein families shotgun data, using similarity-based clustering set filters extract bona fide families. We applied method 17 virus-enriched libraries...
Myalgic encephalomyelitis (ME) previously also known as chronic fatigue syndrome is a heterogeneous, debilitating of unknown etiology responsible for long-lasting disability in millions patients worldwide. The most well-known symptom ME post-exertional malaise, but many experience autonomic dysregulation, cranial nerve dysfunction and signs immune system activation. Many report sudden onset disease following an infection. brainstem suspected focal point pathogenesis with structural...
Background The use of in vitro models to unravel the phenotypic characteristics circulating viral variants is key understanding HIV-1 pathogenesis but limited by availability primary isolates from biological samples. However, overall vivo genetic variability within a subject may not be reflected viable population obtained after isolation. Although several studies have tried determine whether populations expanded are representative findings, answer remains unclear due reduced number clonal...
Prostate cancer is a heterogeneous disease with need for new prognostic biomarkers. Human leukocyte antigen (HLA) genes are highly polymorphic central to presentation T-cells. Two alleles, HLA-A*02:01 and HLA-A*24:02, have been associated prognosis in patients diagnosed de novo metastatic prostate cancer. We leveraged the next-generation sequenced cohorts CPC-GENE TCGA-PRAD examine HLA antiviral T-cell receptors recurrence after prostatectomy. Carrying (111/229; 48% of patients) was...
Background Technically, HIV-1 tropism can be evaluated in plasma or peripheral blood mononuclear cells (PBMCs). However, only testing of has been validated as a tool to predict virological response CCR5 antagonists clinical trials. The preferable strategy subjects with undetectable viremia, whom is not feasible, remains uncertain. Methods & Results We designed proof-of-concept study including 30 chronically HIV-1-infected individuals who achieved RNA <50 copies/mL during at least 2 years...