A5005654823- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Inflammasome and immune disorders
- Malaria Research and Control
- Immune Cell Function and Interaction
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Mosquito-borne diseases and control
- interferon and immune responses
- T-cell and B-cell Immunology
- Endoplasmic Reticulum Stress and Disease
- Complement system in diseases
- Vector-borne infectious diseases
- Parasitic Infections and Diagnostics
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Pineapple and bromelain studies
- Advanced biosensing and bioanalysis techniques
- Phytase and its Applications
- Toxin Mechanisms and Immunotoxins
- Viral Infections and Immunology Research
- Toxoplasma gondii Research Studies
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Hepatitis Viruses Studies and Epidemiology
Universidade Federal de Minas Gerais
2008-2025
Fundação Oswaldo Cruz
2014-2024
Boston Children's Hospital
2019-2023
Harvard University
2020-2023
Faculdades Oswaldo Cruz
2023
Boston Children's Museum
2019
Highlights•Neutrophils are highly activated during P. vivax infection•P. infection induces a high frequency of low-density granulocytes•Neutrophils from malaria patients display type I IFN transcriptional signature•IFNAR and caspase 1 induce neutrophil recruitment liver damage in rodent malariaSummaryNeutrophils the most abundant leukocyte population bloodstream, primary compartment Plasmodium sp. infection. However, role these polymorphonuclear cells mediating either resistance or...
SARS-CoV-2 causes acute respiratory distress that can progress to multiorgan failure and death in a minority of patients. Although severe COVID-19 disease is linked exuberant inflammation, how triggers inflammation not understood. Monocytes macrophages are sentinel immune cells the blood tissue, respectively, sense invasive infection form inflammasomes activate caspase-1 gasdermin D (GSDMD) pores, leading inflammatory (pyroptosis) processing release IL-1 family cytokines, potent mediators....
SARS-CoV-2 causes acute respiratory distress that can progress to multiorgan failure and death in some patients. Although severe COVID-19 disease is linked exuberant inflammation, how triggers inflammation not understood. Monocytes are sentinel blood cells sense invasive infection form inflammasomes activate caspase-1 gasdermin D (GSDMD) pores, leading inflammatory (pyroptosis) processing release of IL-1 family cytokines, potent mediators. Here we show ~10% monocytes patients dying infected...
Secreted and surface-displayed carbohydrates are essential for virulence viability of many parasites, including immune system evasion. We have identified the α-Gal trisaccharide epitope on surface protozoan parasites Leishmania infantum amazonensis, etiological agents visceral cutaneous leishmaniasis, respectively, with latter bearing larger amounts than former. A polyvalent conjugate immunogenic Qβ virus-like particle was tested as a vaccine against infection in C57BL/6...
Significance Immunotherapy benefits some aggressive breast cancers, but many tumors do not respond to checkpoint blockade. Novel strategies increase cancer immunogenicity are needed improve immunotherapy. Here, we used epithelial cell adhesion molecule (EpCAM) aptamer-linked small-interfering RNA chimeras (AsiC) selectively knock down genes in mouse cancers induce tumor neoantigens or overcome immune evasion. Individual gene knockdown markedly delayed growth and enhanced antitumor immunity....
Trypanosoma cruzi, the protozoan that causes Chagas disease, has a complex life cycle involving several morphologically and biochemically distinct stages establish intricate interactions with various insect mammalian hosts. It also heterogeneous population structure comprising strains properties such as virulence, sensitivity to drugs, antigenic profile tissue tropism. We present comparative transcriptome analysis of two cloned T. cruzi display contrasting virulence phenotypes in animal...
The COVID-19 pandemic highlighted the global necessity to develop fast, affordable, and user-friendly diagnostic alternatives. Alongside recognized tests such as ELISA, nanotechnologies have since been explored for direct indirect diagnosis of SARS-CoV-2, etiological agent COVID-19. Accordingly, in this work, we report a method detect anti-SARS-CoV-2 antibodies based on graphene-based field-effect transistors (GFETs), using nanostructured platform graphene with added gold nanorods (GNRs)...
Abstract TLR9 is critical in parasite recognition and host resistance to experimental infection with Trypanosoma cruzi. However, no information available regarding nucleotide sequences cellular events involved on T. cruzi by TLR9. In silico wide analysis associated vitro screening of synthetic oligonucleotides demonstrates that the retrotransposon VIPER elements mucin-like glycoprotein (TcMUC) genes genome are highly enriched for CpG motifs immunostimulatory mouse human TLR9, respectively....
One of the main challenges in cancer research is development vaccines that induce effective and long-lived protective immunity against tumors. Significant progress has been made identifying members testis antigen family as potential vaccine candidates. However, an ideal form for delivery induces robust sustainable antigen-specific T-cell responses, particular CD8 + T lymphocytes, remains to be developed. Here we report use a recombinant nonpathogenic clone Trypanosoma cruzi vector vigorous...
The function and regulation of the immune response triggered during malaria is complex poorly understood, there a particular paucity studies conducted in humans infected with Plasmodium vivax. While it has been proposed that T-cell-effector responses are crucial for protection against blood-stage mice, mechanisms behind this remain understood. Experimental models have shown regulatory molecules, cytotoxic T-lymphocyte attenuator-4 (CTLA-4), lymphocyte activation gene-3 (LAG-3), programmed...
Abstract Immunization with the Amastigote Surface Protein-2 (ASP-2) and Trans -sialidase (TS) antigens either in form of recombinant protein, encoded plasmids or human adenovirus 5 (hAd5) confers robust protection against various lineages Trypanosoma cruzi . Herein we generated a chimeric protein containing most immunogenic regions for T B cells from TS ASP-2 (TRASP) evaluated its immunogenicity comparison our standard protocol heterologous prime-boost using hAd5. Mice immunized TRASP...
Severe COVID-19 is characterized by persistent lung inflammation, inflammatory cytokine production, viral RNA, and sustained interferon (IFN) response all of which are recapitulated required for pathology in the SARS-CoV-2 infected MISTRG6-hACE2 humanized mouse model with a human immune system 1-20 . Blocking either replication Remdesivir 21-23 or downstream IFN stimulated cascade anti-IFNAR2 vivo chronic stages disease attenuated overactive immune-inflammatory response, especially...
Studies have demonstrated the protective role of antibodies against malaria. Young children are known to be particularly vulnerable malaria, pointing evolution naturally acquired clinical immunity over time. However, whether changes in antibody functionality track with acquisition malaria remains incompletely understood.
Immunological adjuvants that induce T cell-mediate immunity (TCMI) with the least side effects are needed for development of human vaccines. Glycoinositolphospholipids (GIPL) and CpGs oligodeoxynucleotides (CpG ODNs) derived from protozoa parasite Trypanosoma cruzi potent pro-inflammatory reaction through activation Toll-Like Receptor (TLR)4 TLR9, respectively. Here, using mouse models, we tested T. TLR agonists as immunological in an antitumor vaccine. For comparison, used well-established...