A5064750311- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Molecular Biology Techniques and Applications
- Cancer-related molecular mechanisms research
- Genetic and Kidney Cyst Diseases
- Protein purification and stability
- Protein Kinase Regulation and GTPase Signaling
- Parathyroid Disorders and Treatments
- Transgenic Plants and Applications
- Cardiac electrophysiology and arrhythmias
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Genetic Syndromes and Imprinting
- Genomics and Phylogenetic Studies
Pfizer (United States)
2018-2020
Brandeis University
2015-2018
Large-scale transient expression in mammalian cells is a rapid protein production technology often used to shorten overall timelines for biotherapeutics drug discovery. In this study we demonstrate Chinese hamster ovary (CHO) host (ExpiCHO-S™) cell line capable of achieving high recombinant antibody titers, comparable levels obtained using human embryonic kidney (HEK) 293 cells. For some antibodies, ExpiCHO-S™ generated materials with better titers and improved quality characteristics (i.e.,...
The fortuitously discovered antiaging membrane protein αKlotho (Klotho) is highly expressed in the kidney, and deletion of Klotho gene mice causes a phenotype strikingly similar to that chronic kidney disease (CKD). functions as co-receptor for fibroblast growth factor 23 (FGF23) signaling, whereas its shed extracellular domain, soluble (sKlotho), carrying glycosidase activity, humoral regulates renal health. Low sKlotho CKD associated with progression, supplementation has emerged potential...
Abstract Summary: Gene targeting is a protocol for introducing mutation to specific gene in an organism. Because of the importance vivo assessment function and modeling human diseases, this technique has been widely adopted generate large number mutant mouse models. Due recent breakthroughs high-throughput sequencing technologies, RNA-Seq experiments have performed on many these models, leading hundreds publicly available datasets. To facilitate reuse datasets, we collected associated...
Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a well-characterized, abundant that regulates diverse set of functions in tissue-specific manner. For example, heart muscle, CaMKII Ca2+ homeostasis, whereas neurons, activity-dependent dendritic remodeling and long-term potentiation (LTP), neurobiological correlate learning memory. Previously, we identified the GTPase Rem2 as critical regulator dendrite branching homeostatic plasticity vertebrate nervous system. Here, report directly...
This study has employed mammalian transient expression systems to generate afucosylated antibodies and antibody Fc mutants for rapid candidate screening in discovery early development. While chemical treatment with the fucose analogue 2-fluoro-peracetyl-fucose during only partially produced N-glycans, genetic inactivation of FUT8 gene ExpiCHO-S™ by CRISPR/Cas9 enabled production fully antibodies. Human IgG1 murine IgG2a generated ExpiCHOfut8KO cell line possessed a 8-to-11-fold enhanced...
Abstract CaMKII is a well-characterized, abundant protein kinase that regulates diverse set of functions in tissue specific manner. For example, heart muscle, Ca 2+ homeostasis while neurons activity-dependent dendritic remodeling and Long Term Potentiation (LTP), biological correlate learning memory. Previously, we identified the noncanonical GTPase Rem2 as critical regulator dendrite branching synapse formation vertebrate nervous system. Here, report directly interacts with potently...